Experiment: MS_media with m-arsenite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3082 and EX28DRAFT_3083 are separated by 226 nucleotides EX28DRAFT_3083 and EX28DRAFT_3084 are separated by 60 nucleotides EX28DRAFT_3084 and EX28DRAFT_3085 are separated by 10 nucleotides EX28DRAFT_3085 and EX28DRAFT_3086 are separated by 105 nucleotides
EX28DRAFT_3082: EX28DRAFT_3082 - Uncharacterized conserved protein, at 394,203 to 395,504
_3082
EX28DRAFT_3083: EX28DRAFT_3083 - SH3 domain protein, at 395,731 to 396,354
_3083
EX28DRAFT_3084: EX28DRAFT_3084 - tRNA nucleotidyltransferase/poly(A) polymerase, at 396,415 to 397,656
_3084
EX28DRAFT_3085: EX28DRAFT_3085 - undecaprenyl-diphosphatase UppP, at 397,667 to 398,488
_3085
EX28DRAFT_3086: EX28DRAFT_3086 - dihydroneopterin aldolase, at 398,594 to 398,962
_3086
Position (kb)
396
397
398 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 395.432 kb on + strand at 395.485 kb on - strand at 395.574 kb on + strand at 395.575 kb on - strand at 395.575 kb on - strand at 395.578 kb on + strand at 395.578 kb on + strand at 395.579 kb on - strand at 395.579 kb on - strand at 395.579 kb on - strand at 395.579 kb on - strand at 395.581 kb on + strand at 395.582 kb on - strand at 395.582 kb on - strand at 395.582 kb on - strand at 395.625 kb on - strand at 395.632 kb on + strand at 395.699 kb on + strand at 395.699 kb on + strand at 395.700 kb on - strand at 395.711 kb on - strand at 395.723 kb on - strand at 395.729 kb on + strand at 395.730 kb on - strand at 395.740 kb on - strand at 395.760 kb on + strand at 395.761 kb on - strand at 395.765 kb on + strand at 395.766 kb on - strand at 395.840 kb on + strand, within EX28DRAFT_3083 at 395.845 kb on - strand, within EX28DRAFT_3083 at 395.845 kb on - strand, within EX28DRAFT_3083 at 395.860 kb on - strand, within EX28DRAFT_3083 at 396.197 kb on + strand, within EX28DRAFT_3083 at 396.255 kb on + strand, within EX28DRAFT_3083 at 396.256 kb on - strand, within EX28DRAFT_3083 at 396.256 kb on - strand, within EX28DRAFT_3083 at 396.256 kb on - strand, within EX28DRAFT_3083 at 396.256 kb on - strand, within EX28DRAFT_3083 at 396.259 kb on + strand, within EX28DRAFT_3083 at 396.260 kb on - strand, within EX28DRAFT_3083 at 396.352 kb on + strand at 396.352 kb on + strand at 396.353 kb on - strand at 396.375 kb on + strand at 396.375 kb on + strand at 396.375 kb on + strand at 396.375 kb on + strand at 396.376 kb on - strand at 396.376 kb on - strand at 396.376 kb on - strand at 396.379 kb on + strand at 396.380 kb on - strand at 396.380 kb on - strand at 397.683 kb on + strand at 397.683 kb on + strand at 397.683 kb on + strand at 397.735 kb on + strand at 397.736 kb on - strand at 397.842 kb on + strand, within EX28DRAFT_3085 at 397.849 kb on + strand, within EX28DRAFT_3085 at 397.850 kb on - strand, within EX28DRAFT_3085 at 397.850 kb on - strand, within EX28DRAFT_3085 at 397.852 kb on - strand, within EX28DRAFT_3085 at 397.852 kb on - strand, within EX28DRAFT_3085 at 398.014 kb on + strand, within EX28DRAFT_3085 at 398.028 kb on + strand, within EX28DRAFT_3085 at 398.029 kb on - strand, within EX28DRAFT_3085 at 398.096 kb on + strand, within EX28DRAFT_3085 at 398.097 kb on - strand, within EX28DRAFT_3085 at 398.118 kb on + strand, within EX28DRAFT_3085 at 398.119 kb on - strand, within EX28DRAFT_3085 at 398.206 kb on + strand, within EX28DRAFT_3085 at 398.207 kb on - strand, within EX28DRAFT_3085 at 398.320 kb on + strand, within EX28DRAFT_3085 at 398.398 kb on + strand, within EX28DRAFT_3085 at 398.399 kb on - strand, within EX28DRAFT_3085 at 398.454 kb on - strand at 398.490 kb on + strand at 398.491 kb on - strand at 398.491 kb on - strand at 398.491 kb on - strand at 398.491 kb on - strand at 398.546 kb on + strand at 398.546 kb on + strand at 398.546 kb on + strand at 398.547 kb on - strand at 398.547 kb on - strand at 398.566 kb on - strand at 398.570 kb on + strand at 398.596 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM remove 395,432 + -3.7 395,485 - -2.3 395,574 + -0.3 395,575 - -0.3 395,575 - -2.4 395,578 + -0.9 395,578 + +0.2 395,579 - +0.0 395,579 - -0.2 395,579 - +0.2 395,579 - -0.3 395,581 + -1.1 395,582 - -0.1 395,582 - +0.2 395,582 - +1.3 395,625 - +0.5 395,632 + -0.5 395,699 + +0.4 395,699 + -1.4 395,700 - +0.0 395,711 - +0.3 395,723 - -0.2 395,729 + +0.2 395,730 - -2.7 395,740 - -0.1 395,760 + +0.5 395,761 - -0.1 395,765 + -0.4 395,766 - +0.2 395,840 + EX28DRAFT_3083 0.17 -0.3 395,845 - EX28DRAFT_3083 0.18 +0.3 395,845 - EX28DRAFT_3083 0.18 +0.4 395,860 - EX28DRAFT_3083 0.21 -1.4 396,197 + EX28DRAFT_3083 0.75 +0.1 396,255 + EX28DRAFT_3083 0.84 +0.1 396,256 - EX28DRAFT_3083 0.84 -0.9 396,256 - EX28DRAFT_3083 0.84 -0.3 396,256 - EX28DRAFT_3083 0.84 +0.5 396,256 - EX28DRAFT_3083 0.84 +0.5 396,259 + EX28DRAFT_3083 0.85 +0.1 396,260 - EX28DRAFT_3083 0.85 -1.3 396,352 + +1.2 396,352 + +0.1 396,353 - -0.4 396,375 + +1.3 396,375 + -0.1 396,375 + -0.3 396,375 + +0.0 396,376 - -3.0 396,376 - -1.4 396,376 - -1.1 396,379 + -0.3 396,380 - -0.7 396,380 - -0.3 397,683 + -0.3 397,683 + +0.0 397,683 + +0.1 397,735 + -0.1 397,736 - -1.3 397,842 + EX28DRAFT_3085 0.21 -1.2 397,849 + EX28DRAFT_3085 0.22 -0.5 397,850 - EX28DRAFT_3085 0.22 -0.7 397,850 - EX28DRAFT_3085 0.22 +0.3 397,852 - EX28DRAFT_3085 0.23 -1.1 397,852 - EX28DRAFT_3085 0.23 -0.8 398,014 + EX28DRAFT_3085 0.42 -0.2 398,028 + EX28DRAFT_3085 0.44 -3.1 398,029 - EX28DRAFT_3085 0.44 +0.9 398,096 + EX28DRAFT_3085 0.52 +0.5 398,097 - EX28DRAFT_3085 0.52 -0.4 398,118 + EX28DRAFT_3085 0.55 +0.3 398,119 - EX28DRAFT_3085 0.55 -1.2 398,206 + EX28DRAFT_3085 0.66 +0.3 398,207 - EX28DRAFT_3085 0.66 +0.4 398,320 + EX28DRAFT_3085 0.79 -1.0 398,398 + EX28DRAFT_3085 0.89 -0.3 398,399 - EX28DRAFT_3085 0.89 -0.2 398,454 - -0.4 398,490 + +0.6 398,491 - -0.0 398,491 - -0.1 398,491 - +0.5 398,491 - -0.3 398,546 + +0.4 398,546 + -2.9 398,546 + +0.9 398,547 - -0.4 398,547 - -2.0 398,566 - -0.0 398,570 + -0.4 398,596 - +0.5
Or see this region's nucleotide sequence