Experiment: MS_media with m-arsenite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3001 and EX28DRAFT_3002 are separated by 90 nucleotides EX28DRAFT_3002 and EX28DRAFT_3003 overlap by 23 nucleotides EX28DRAFT_3003 and EX28DRAFT_3004 overlap by 4 nucleotides
EX28DRAFT_3001: EX28DRAFT_3001 - nucleoside transporter, at 309,284 to 310,540
_3001
EX28DRAFT_3002: EX28DRAFT_3002 - triphosphoribosyl-dephospho-CoA synthase CitG, at 310,631 to 311,434
_3002
EX28DRAFT_3003: EX28DRAFT_3003 - holo-ACP synthase CitX, at 311,412 to 311,951
_3003
EX28DRAFT_3004: EX28DRAFT_3004 - citrate lyase, alpha subunit, at 311,948 to 313,471
_3004
Position (kb) 311
312 Strain fitness (log2 ratio) -2
-1
0
1 at 310.493 kb on + strand at 310.494 kb on - strand at 310.494 kb on - strand at 310.538 kb on + strand at 310.538 kb on + strand at 310.611 kb on + strand at 310.632 kb on + strand at 310.723 kb on + strand, within EX28DRAFT_3002 at 310.724 kb on - strand, within EX28DRAFT_3002 at 311.062 kb on + strand, within EX28DRAFT_3002 at 311.093 kb on + strand, within EX28DRAFT_3002 at 311.093 kb on + strand, within EX28DRAFT_3002 at 311.414 kb on - strand at 311.781 kb on - strand, within EX28DRAFT_3003 at 311.852 kb on - strand, within EX28DRAFT_3003 at 312.813 kb on + strand, within EX28DRAFT_3004 at 312.814 kb on - strand, within EX28DRAFT_3004 at 312.814 kb on - strand, within EX28DRAFT_3004
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM remove 310,493 + +0.4 310,494 - +0.2 310,494 - +0.5 310,538 + +0.7 310,538 + +0.4 310,611 + -0.1 310,632 + -0.1 310,723 + EX28DRAFT_3002 0.11 +0.7 310,724 - EX28DRAFT_3002 0.12 -0.1 311,062 + EX28DRAFT_3002 0.54 -2.1 311,093 + EX28DRAFT_3002 0.57 -0.5 311,093 + EX28DRAFT_3002 0.57 +0.5 311,414 - +1.6 311,781 - EX28DRAFT_3003 0.68 +0.2 311,852 - EX28DRAFT_3003 0.81 -0.1 312,813 + EX28DRAFT_3004 0.57 -0.4 312,814 - EX28DRAFT_3004 0.57 -0.8 312,814 - EX28DRAFT_3004 0.57 +0.6
Or see this region's nucleotide sequence