Experiment: MS_media with m-arsenite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2988 and EX28DRAFT_2989 are separated by 17 nucleotides EX28DRAFT_2989 and EX28DRAFT_2990 are separated by 21 nucleotides EX28DRAFT_2990 and EX28DRAFT_2991 overlap by 4 nucleotides EX28DRAFT_2991 and EX28DRAFT_2992 are separated by 3 nucleotides
EX28DRAFT_2988: EX28DRAFT_2988 - pilus retraction protein PilT, at 299,814 to 300,794
_2988
EX28DRAFT_2989: EX28DRAFT_2989 - pyridoxal phosphate enzyme, YggS family, at 300,812 to 301,513
_2989
EX28DRAFT_2990: EX28DRAFT_2990 - Predicted integral membrane protein, at 301,535 to 302,101
_2990
EX28DRAFT_2991: EX28DRAFT_2991 - TIGR00251 family protein, at 302,098 to 302,394
_2991
EX28DRAFT_2992: EX28DRAFT_2992 - non-canonical purine NTP pyrophosphatase, RdgB/HAM1 family, at 302,398 to 302,991
_2992
Position (kb) 300
301
302 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 299.815 kb on + strand at 299.965 kb on + strand, within EX28DRAFT_2988 at 299.965 kb on + strand, within EX28DRAFT_2988 at 299.965 kb on + strand, within EX28DRAFT_2988 at 299.966 kb on - strand, within EX28DRAFT_2988 at 300.308 kb on - strand, within EX28DRAFT_2988 at 300.308 kb on - strand, within EX28DRAFT_2988 at 300.311 kb on + strand, within EX28DRAFT_2988 at 300.312 kb on - strand, within EX28DRAFT_2988 at 300.342 kb on - strand, within EX28DRAFT_2988 at 300.390 kb on - strand, within EX28DRAFT_2988 at 300.639 kb on - strand, within EX28DRAFT_2988 at 300.651 kb on + strand, within EX28DRAFT_2988 at 300.651 kb on + strand, within EX28DRAFT_2988 at 300.741 kb on + strand at 300.742 kb on - strand at 300.756 kb on - strand at 300.762 kb on + strand at 300.767 kb on + strand at 300.768 kb on - strand at 300.795 kb on - strand at 300.795 kb on - strand at 300.799 kb on + strand at 300.799 kb on + strand at 300.800 kb on - strand at 300.949 kb on + strand, within EX28DRAFT_2989 at 300.949 kb on + strand, within EX28DRAFT_2989 at 300.984 kb on - strand, within EX28DRAFT_2989 at 300.984 kb on - strand, within EX28DRAFT_2989 at 301.180 kb on + strand, within EX28DRAFT_2989 at 301.329 kb on - strand, within EX28DRAFT_2989 at 301.365 kb on + strand, within EX28DRAFT_2989 at 301.381 kb on + strand, within EX28DRAFT_2989 at 301.382 kb on - strand, within EX28DRAFT_2989 at 301.393 kb on + strand, within EX28DRAFT_2989 at 301.393 kb on + strand, within EX28DRAFT_2989 at 301.401 kb on + strand, within EX28DRAFT_2989 at 301.432 kb on + strand, within EX28DRAFT_2989 at 301.462 kb on + strand at 301.511 kb on + strand at 301.512 kb on - strand at 301.514 kb on + strand at 301.515 kb on - strand at 301.579 kb on + strand at 301.801 kb on + strand, within EX28DRAFT_2990 at 302.021 kb on + strand, within EX28DRAFT_2990 at 302.022 kb on - strand, within EX28DRAFT_2990 at 302.068 kb on + strand at 302.143 kb on + strand, within EX28DRAFT_2991 at 302.144 kb on - strand, within EX28DRAFT_2991 at 302.144 kb on - strand, within EX28DRAFT_2991 at 302.144 kb on - strand, within EX28DRAFT_2991 at 302.144 kb on - strand, within EX28DRAFT_2991 at 302.145 kb on + strand, within EX28DRAFT_2991 at 302.199 kb on - strand, within EX28DRAFT_2991 at 302.325 kb on + strand, within EX28DRAFT_2991 at 302.326 kb on - strand, within EX28DRAFT_2991 at 302.326 kb on - strand, within EX28DRAFT_2991 at 302.326 kb on - strand, within EX28DRAFT_2991 at 302.326 kb on - strand, within EX28DRAFT_2991 at 302.393 kb on - strand at 302.393 kb on - strand at 302.460 kb on - strand, within EX28DRAFT_2992 at 302.460 kb on - strand, within EX28DRAFT_2992 at 302.460 kb on - strand, within EX28DRAFT_2992
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM remove 299,815 + -0.2 299,965 + EX28DRAFT_2988 0.15 -0.1 299,965 + EX28DRAFT_2988 0.15 +0.3 299,965 + EX28DRAFT_2988 0.15 +0.4 299,966 - EX28DRAFT_2988 0.15 +0.6 300,308 - EX28DRAFT_2988 0.50 +0.0 300,308 - EX28DRAFT_2988 0.50 +0.4 300,311 + EX28DRAFT_2988 0.51 -0.2 300,312 - EX28DRAFT_2988 0.51 +0.1 300,342 - EX28DRAFT_2988 0.54 -0.6 300,390 - EX28DRAFT_2988 0.59 +0.1 300,639 - EX28DRAFT_2988 0.84 -0.0 300,651 + EX28DRAFT_2988 0.85 +0.1 300,651 + EX28DRAFT_2988 0.85 -0.3 300,741 + +0.0 300,742 - -1.1 300,756 - -0.3 300,762 + +0.3 300,767 + +0.9 300,768 - -0.7 300,795 - -1.6 300,795 - +0.3 300,799 + -0.6 300,799 + -0.5 300,800 - -0.7 300,949 + EX28DRAFT_2989 0.20 -1.2 300,949 + EX28DRAFT_2989 0.20 -1.4 300,984 - EX28DRAFT_2989 0.25 -0.6 300,984 - EX28DRAFT_2989 0.25 -2.0 301,180 + EX28DRAFT_2989 0.52 -2.5 301,329 - EX28DRAFT_2989 0.74 -2.9 301,365 + EX28DRAFT_2989 0.79 -0.5 301,381 + EX28DRAFT_2989 0.81 -2.1 301,382 - EX28DRAFT_2989 0.81 -1.6 301,393 + EX28DRAFT_2989 0.83 -1.8 301,393 + EX28DRAFT_2989 0.83 -3.7 301,401 + EX28DRAFT_2989 0.84 +0.0 301,432 + EX28DRAFT_2989 0.88 -2.0 301,462 + -2.3 301,511 + +1.9 301,512 - +0.4 301,514 + +0.2 301,515 - -0.1 301,579 + +0.5 301,801 + EX28DRAFT_2990 0.47 +0.4 302,021 + EX28DRAFT_2990 0.86 +0.3 302,022 - EX28DRAFT_2990 0.86 +0.1 302,068 + +0.4 302,143 + EX28DRAFT_2991 0.15 +0.3 302,144 - EX28DRAFT_2991 0.15 -0.4 302,144 - EX28DRAFT_2991 0.15 -3.7 302,144 - EX28DRAFT_2991 0.15 +0.7 302,144 - EX28DRAFT_2991 0.15 -0.3 302,145 + EX28DRAFT_2991 0.16 -0.9 302,199 - EX28DRAFT_2991 0.34 -2.8 302,325 + EX28DRAFT_2991 0.76 +1.2 302,326 - EX28DRAFT_2991 0.77 -0.3 302,326 - EX28DRAFT_2991 0.77 +0.1 302,326 - EX28DRAFT_2991 0.77 -0.3 302,326 - EX28DRAFT_2991 0.77 +2.1 302,393 - +0.7 302,393 - +0.5 302,460 - EX28DRAFT_2992 0.10 +1.0 302,460 - EX28DRAFT_2992 0.10 -0.2 302,460 - EX28DRAFT_2992 0.10 -0.1
Or see this region's nucleotide sequence