Experiment: MS_media with m-arsenite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2892 and EX28DRAFT_2893 are separated by 212 nucleotides EX28DRAFT_2893 and EX28DRAFT_2894 are separated by 34 nucleotides
EX28DRAFT_2892: EX28DRAFT_2892 - N-acetylmuramoyl-L-alanine amidase, at 195,589 to 196,857
_2892
EX28DRAFT_2893: EX28DRAFT_2893 - N-acetylglutamate synthase (EC 2.3.1.1), at 197,070 to 198,401
_2893
EX28DRAFT_2894: EX28DRAFT_2894 - DNA helicase/exodeoxyribonuclease V, alpha subunit (EC 3.1.11.5), at 198,436 to 200,256
_2894
Position (kb) 197
198
199 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 196.119 kb on + strand, within EX28DRAFT_2892 at 196.137 kb on + strand, within EX28DRAFT_2892 at 196.137 kb on + strand, within EX28DRAFT_2892 at 196.140 kb on - strand, within EX28DRAFT_2892 at 196.140 kb on - strand, within EX28DRAFT_2892 at 196.149 kb on + strand, within EX28DRAFT_2892 at 196.361 kb on + strand, within EX28DRAFT_2892 at 196.395 kb on + strand, within EX28DRAFT_2892 at 196.440 kb on + strand, within EX28DRAFT_2892 at 196.441 kb on - strand, within EX28DRAFT_2892 at 196.664 kb on + strand, within EX28DRAFT_2892 at 196.665 kb on - strand, within EX28DRAFT_2892 at 196.704 kb on - strand, within EX28DRAFT_2892 at 196.704 kb on - strand, within EX28DRAFT_2892 at 196.801 kb on + strand at 196.874 kb on - strand at 196.928 kb on + strand at 196.929 kb on - strand at 196.929 kb on - strand at 196.949 kb on - strand at 197.084 kb on + strand at 197.085 kb on - strand at 197.085 kb on - strand at 197.085 kb on - strand at 197.125 kb on - strand at 197.256 kb on + strand, within EX28DRAFT_2893 at 197.256 kb on + strand, within EX28DRAFT_2893 at 197.257 kb on - strand, within EX28DRAFT_2893 at 197.257 kb on - strand, within EX28DRAFT_2893 at 197.423 kb on + strand, within EX28DRAFT_2893 at 197.423 kb on + strand, within EX28DRAFT_2893 at 197.549 kb on + strand, within EX28DRAFT_2893 at 197.549 kb on + strand, within EX28DRAFT_2893 at 197.550 kb on - strand, within EX28DRAFT_2893 at 197.877 kb on + strand, within EX28DRAFT_2893 at 198.189 kb on - strand, within EX28DRAFT_2893 at 198.354 kb on + strand at 198.354 kb on + strand at 198.354 kb on + strand at 198.355 kb on - strand at 198.355 kb on - strand at 198.355 kb on - strand at 198.360 kb on + strand at 198.360 kb on + strand at 198.361 kb on - strand at 198.361 kb on - strand at 198.361 kb on - strand at 198.560 kb on + strand at 198.560 kb on + strand at 198.560 kb on + strand at 198.561 kb on - strand at 198.580 kb on + strand at 198.580 kb on + strand at 198.580 kb on + strand at 198.580 kb on + strand at 198.642 kb on + strand, within EX28DRAFT_2894 at 198.643 kb on - strand, within EX28DRAFT_2894 at 198.948 kb on - strand, within EX28DRAFT_2894 at 198.982 kb on - strand, within EX28DRAFT_2894 at 198.995 kb on - strand, within EX28DRAFT_2894 at 198.995 kb on - strand, within EX28DRAFT_2894 at 199.049 kb on + strand, within EX28DRAFT_2894 at 199.084 kb on - strand, within EX28DRAFT_2894 at 199.313 kb on + strand, within EX28DRAFT_2894
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM remove 196,119 + EX28DRAFT_2892 0.42 +0.1 196,137 + EX28DRAFT_2892 0.43 +0.1 196,137 + EX28DRAFT_2892 0.43 +0.2 196,140 - EX28DRAFT_2892 0.43 +0.3 196,140 - EX28DRAFT_2892 0.43 -0.2 196,149 + EX28DRAFT_2892 0.44 +0.1 196,361 + EX28DRAFT_2892 0.61 -0.7 196,395 + EX28DRAFT_2892 0.64 +0.8 196,440 + EX28DRAFT_2892 0.67 +0.2 196,441 - EX28DRAFT_2892 0.67 -0.2 196,664 + EX28DRAFT_2892 0.85 -0.1 196,665 - EX28DRAFT_2892 0.85 -0.5 196,704 - EX28DRAFT_2892 0.88 -0.4 196,704 - EX28DRAFT_2892 0.88 -0.1 196,801 + +0.6 196,874 - -1.3 196,928 + +0.3 196,929 - -0.7 196,929 - -0.6 196,949 - -0.9 197,084 + +0.1 197,085 - -1.6 197,085 - -4.5 197,085 - +1.1 197,125 - -0.5 197,256 + EX28DRAFT_2893 0.14 -1.6 197,256 + EX28DRAFT_2893 0.14 -2.0 197,257 - EX28DRAFT_2893 0.14 +0.8 197,257 - EX28DRAFT_2893 0.14 -4.6 197,423 + EX28DRAFT_2893 0.27 -3.5 197,423 + EX28DRAFT_2893 0.27 -1.6 197,549 + EX28DRAFT_2893 0.36 -2.4 197,549 + EX28DRAFT_2893 0.36 -2.3 197,550 - EX28DRAFT_2893 0.36 -2.2 197,877 + EX28DRAFT_2893 0.61 -2.9 198,189 - EX28DRAFT_2893 0.84 +0.5 198,354 + -2.6 198,354 + -3.5 198,354 + -2.0 198,355 - -1.8 198,355 - -2.9 198,355 - -2.2 198,360 + -2.1 198,360 + -2.4 198,361 - -1.2 198,361 - -0.8 198,361 - -0.2 198,560 + +3.1 198,560 + -1.8 198,560 + +0.9 198,561 - -0.7 198,580 + +1.2 198,580 + +0.2 198,580 + -0.1 198,580 + -2.3 198,642 + EX28DRAFT_2894 0.11 -0.4 198,643 - EX28DRAFT_2894 0.11 +0.2 198,948 - EX28DRAFT_2894 0.28 -0.8 198,982 - EX28DRAFT_2894 0.30 -0.2 198,995 - EX28DRAFT_2894 0.31 -0.2 198,995 - EX28DRAFT_2894 0.31 -1.0 199,049 + EX28DRAFT_2894 0.34 -0.0 199,084 - EX28DRAFT_2894 0.36 +0.1 199,313 + EX28DRAFT_2894 0.48 +0.3
Or see this region's nucleotide sequence