Experiment: MS_media with m-arsenite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2872 and EX28DRAFT_2873 overlap by 4 nucleotides EX28DRAFT_2873 and EX28DRAFT_2874 are separated by 55 nucleotides EX28DRAFT_2874 and EX28DRAFT_2875 are separated by 218 nucleotides EX28DRAFT_2875 and EX28DRAFT_2876 are separated by 69 nucleotides
EX28DRAFT_2872: EX28DRAFT_2872 - Pseudouridylate synthases, 23S RNA-specific, at 179,364 to 180,140
_2872
EX28DRAFT_2873: EX28DRAFT_2873 - Uncharacterized protein conserved in bacteria, at 180,137 to 180,466
_2873
EX28DRAFT_2874: EX28DRAFT_2874 - RNA, at 180,522 to 180,878
_2874
EX28DRAFT_2875: EX28DRAFT_2875 - Syd protein (SUKH-2), at 181,097 to 181,642
_2875
EX28DRAFT_2876: EX28DRAFT_2876 - 7-cyano-7-deazaguanine reductase, at 181,712 to 182,554
_2876
Position (kb) 181
182 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 180.302 kb on + strand, within EX28DRAFT_2873 at 180.466 kb on + strand at 180.467 kb on - strand at 180.469 kb on + strand at 180.503 kb on + strand at 180.503 kb on + strand at 180.504 kb on - strand at 180.543 kb on - strand at 180.893 kb on + strand at 180.893 kb on + strand at 180.893 kb on + strand at 180.894 kb on - strand at 180.919 kb on - strand at 180.952 kb on - strand at 180.952 kb on - strand at 181.063 kb on - strand at 181.099 kb on - strand at 181.213 kb on + strand, within EX28DRAFT_2875 at 181.264 kb on + strand, within EX28DRAFT_2875 at 181.355 kb on - strand, within EX28DRAFT_2875 at 181.401 kb on + strand, within EX28DRAFT_2875 at 181.495 kb on + strand, within EX28DRAFT_2875 at 181.501 kb on - strand, within EX28DRAFT_2875 at 181.520 kb on - strand, within EX28DRAFT_2875 at 181.535 kb on + strand, within EX28DRAFT_2875 at 181.612 kb on + strand at 181.683 kb on + strand at 181.683 kb on + strand at 181.684 kb on - strand at 181.689 kb on + strand at 181.689 kb on + strand at 181.689 kb on + strand at 181.690 kb on - strand at 181.705 kb on - strand at 181.887 kb on - strand, within EX28DRAFT_2876 at 181.887 kb on - strand, within EX28DRAFT_2876 at 181.887 kb on - strand, within EX28DRAFT_2876 at 181.952 kb on + strand, within EX28DRAFT_2876 at 181.953 kb on - strand, within EX28DRAFT_2876 at 181.987 kb on + strand, within EX28DRAFT_2876 at 181.988 kb on - strand, within EX28DRAFT_2876 at 182.065 kb on - strand, within EX28DRAFT_2876 at 182.187 kb on - strand, within EX28DRAFT_2876 at 182.411 kb on - strand, within EX28DRAFT_2876 at 182.469 kb on - strand, within EX28DRAFT_2876 at 182.555 kb on + strand at 182.558 kb on - strand at 182.561 kb on + strand at 182.562 kb on - strand at 182.573 kb on - strand at 182.629 kb on - strand at 182.629 kb on - strand at 182.629 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM remove 180,302 + EX28DRAFT_2873 0.50 -0.4 180,466 + -0.3 180,467 - -0.8 180,469 + +0.2 180,503 + +0.1 180,503 + -0.3 180,504 - -1.1 180,543 - +0.3 180,893 + +0.1 180,893 + -0.3 180,893 + -0.1 180,894 - -0.2 180,919 - +0.6 180,952 - +0.8 180,952 - +0.1 181,063 - -1.5 181,099 - +0.7 181,213 + EX28DRAFT_2875 0.21 -0.3 181,264 + EX28DRAFT_2875 0.31 +0.4 181,355 - EX28DRAFT_2875 0.47 +0.6 181,401 + EX28DRAFT_2875 0.56 -0.7 181,495 + EX28DRAFT_2875 0.73 -2.8 181,501 - EX28DRAFT_2875 0.74 -0.3 181,520 - EX28DRAFT_2875 0.77 -1.0 181,535 + EX28DRAFT_2875 0.80 -0.2 181,612 + +1.2 181,683 + -0.4 181,683 + +0.2 181,684 - +0.3 181,689 + -0.4 181,689 + -0.2 181,689 + -0.1 181,690 - +0.8 181,705 - +0.0 181,887 - EX28DRAFT_2876 0.21 +0.5 181,887 - EX28DRAFT_2876 0.21 -0.4 181,887 - EX28DRAFT_2876 0.21 +0.8 181,952 + EX28DRAFT_2876 0.28 +0.2 181,953 - EX28DRAFT_2876 0.29 +0.5 181,987 + EX28DRAFT_2876 0.33 +0.7 181,988 - EX28DRAFT_2876 0.33 -0.4 182,065 - EX28DRAFT_2876 0.42 -0.5 182,187 - EX28DRAFT_2876 0.56 +2.2 182,411 - EX28DRAFT_2876 0.83 +1.4 182,469 - EX28DRAFT_2876 0.90 -0.1 182,555 + -0.1 182,558 - +0.1 182,561 + -2.0 182,562 - +0.3 182,573 - -0.9 182,629 - -1.6 182,629 - -0.6 182,629 - -1.6
Or see this region's nucleotide sequence