Experiment: MS_media with m-arsenite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2843 and EX28DRAFT_2844 are separated by 19 nucleotides EX28DRAFT_2844 and EX28DRAFT_2845 are separated by 166 nucleotides EX28DRAFT_2845 and EX28DRAFT_2846 are separated by 58 nucleotides EX28DRAFT_2846 and EX28DRAFT_2847 overlap by 1 nucleotides
EX28DRAFT_2843: EX28DRAFT_2843 - 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase, at 144,947 to 145,657
_2843
EX28DRAFT_2844: EX28DRAFT_2844 - Septum formation initiator, at 145,677 to 145,988
_2844
EX28DRAFT_2845: EX28DRAFT_2845 - Protein of unknown function (DUF3561), at 146,155 to 146,481
_2845
EX28DRAFT_2846: EX28DRAFT_2846 - adenylyl-sulfate kinase, at 146,540 to 147,145
_2846
EX28DRAFT_2847: EX28DRAFT_2847 - sulfate adenylyltransferase, large subunit, at 147,145 to 148,569
_2847
Position (kb) 146
147
148 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 145.635 kb on - strand at 146.039 kb on - strand at 146.120 kb on + strand at 146.120 kb on + strand at 146.145 kb on - strand at 146.156 kb on + strand at 146.226 kb on - strand, within EX28DRAFT_2845 at 146.312 kb on + strand, within EX28DRAFT_2845 at 146.313 kb on - strand, within EX28DRAFT_2845 at 146.462 kb on + strand at 146.462 kb on + strand at 146.463 kb on - strand at 146.463 kb on - strand at 146.481 kb on + strand at 146.482 kb on - strand at 146.485 kb on + strand at 146.485 kb on + strand at 146.485 kb on + strand at 146.486 kb on - strand at 146.486 kb on - strand at 146.580 kb on - strand at 146.580 kb on - strand at 146.588 kb on + strand at 146.649 kb on + strand, within EX28DRAFT_2846 at 146.650 kb on - strand, within EX28DRAFT_2846 at 146.650 kb on - strand, within EX28DRAFT_2846 at 146.704 kb on - strand, within EX28DRAFT_2846 at 146.704 kb on - strand, within EX28DRAFT_2846 at 146.825 kb on - strand, within EX28DRAFT_2846 at 146.967 kb on + strand, within EX28DRAFT_2846 at 146.968 kb on - strand, within EX28DRAFT_2846 at 146.975 kb on + strand, within EX28DRAFT_2846 at 147.147 kb on - strand at 147.274 kb on - strand at 147.315 kb on + strand, within EX28DRAFT_2847 at 147.316 kb on - strand, within EX28DRAFT_2847 at 147.506 kb on + strand, within EX28DRAFT_2847 at 147.506 kb on + strand, within EX28DRAFT_2847 at 147.506 kb on + strand, within EX28DRAFT_2847 at 147.506 kb on + strand, within EX28DRAFT_2847 at 147.506 kb on + strand, within EX28DRAFT_2847 at 147.506 kb on + strand, within EX28DRAFT_2847 at 147.507 kb on - strand, within EX28DRAFT_2847 at 147.507 kb on - strand, within EX28DRAFT_2847 at 147.619 kb on - strand, within EX28DRAFT_2847 at 147.619 kb on - strand, within EX28DRAFT_2847 at 147.748 kb on + strand, within EX28DRAFT_2847 at 147.749 kb on - strand, within EX28DRAFT_2847 at 147.815 kb on + strand, within EX28DRAFT_2847 at 147.816 kb on - strand, within EX28DRAFT_2847 at 147.902 kb on + strand, within EX28DRAFT_2847 at 147.902 kb on + strand, within EX28DRAFT_2847 at 147.902 kb on + strand, within EX28DRAFT_2847 at 147.961 kb on + strand, within EX28DRAFT_2847 at 147.962 kb on - strand, within EX28DRAFT_2847 at 147.972 kb on + strand, within EX28DRAFT_2847 at 148.035 kb on + strand, within EX28DRAFT_2847 at 148.111 kb on - strand, within EX28DRAFT_2847 at 148.111 kb on - strand, within EX28DRAFT_2847
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM remove 145,635 - -1.6 146,039 - -2.1 146,120 + -0.5 146,120 + -0.1 146,145 - -1.9 146,156 + +0.8 146,226 - EX28DRAFT_2845 0.22 -0.5 146,312 + EX28DRAFT_2845 0.48 -1.2 146,313 - EX28DRAFT_2845 0.48 +1.9 146,462 + -0.4 146,462 + +0.0 146,463 - +0.2 146,463 - +1.8 146,481 + +0.4 146,482 - +0.1 146,485 + -0.2 146,485 + -0.1 146,485 + -0.7 146,486 - -0.6 146,486 - -0.6 146,580 - -3.1 146,580 - -3.1 146,588 + -1.7 146,649 + EX28DRAFT_2846 0.18 -1.2 146,650 - EX28DRAFT_2846 0.18 -3.7 146,650 - EX28DRAFT_2846 0.18 -2.1 146,704 - EX28DRAFT_2846 0.27 -1.6 146,704 - EX28DRAFT_2846 0.27 -3.6 146,825 - EX28DRAFT_2846 0.47 -2.5 146,967 + EX28DRAFT_2846 0.70 -2.1 146,968 - EX28DRAFT_2846 0.71 +1.1 146,975 + EX28DRAFT_2846 0.72 -4.3 147,147 - -3.4 147,274 - -1.9 147,315 + EX28DRAFT_2847 0.12 -1.6 147,316 - EX28DRAFT_2847 0.12 -2.3 147,506 + EX28DRAFT_2847 0.25 -0.8 147,506 + EX28DRAFT_2847 0.25 -1.3 147,506 + EX28DRAFT_2847 0.25 -2.2 147,506 + EX28DRAFT_2847 0.25 -0.8 147,506 + EX28DRAFT_2847 0.25 -0.7 147,506 + EX28DRAFT_2847 0.25 -0.6 147,507 - EX28DRAFT_2847 0.25 -1.7 147,507 - EX28DRAFT_2847 0.25 -0.5 147,619 - EX28DRAFT_2847 0.33 -0.6 147,619 - EX28DRAFT_2847 0.33 +0.7 147,748 + EX28DRAFT_2847 0.42 -2.9 147,749 - EX28DRAFT_2847 0.42 -3.3 147,815 + EX28DRAFT_2847 0.47 -3.3 147,816 - EX28DRAFT_2847 0.47 -2.6 147,902 + EX28DRAFT_2847 0.53 -2.4 147,902 + EX28DRAFT_2847 0.53 -0.4 147,902 + EX28DRAFT_2847 0.53 -3.2 147,961 + EX28DRAFT_2847 0.57 -2.4 147,962 - EX28DRAFT_2847 0.57 -0.8 147,972 + EX28DRAFT_2847 0.58 -2.1 148,035 + EX28DRAFT_2847 0.62 -2.7 148,111 - EX28DRAFT_2847 0.68 -2.4 148,111 - EX28DRAFT_2847 0.68 -1.8
Or see this region's nucleotide sequence