Experiment: MS_media with m-arsenite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2837 and EX28DRAFT_2838 are separated by 59 nucleotides EX28DRAFT_2838 and EX28DRAFT_2839 are separated by 116 nucleotides EX28DRAFT_2839 and EX28DRAFT_2840 overlap by 7 nucleotides
EX28DRAFT_2837: EX28DRAFT_2837 - RNA polymerase, sigma 38 subunit, RpoS, at 139,767 to 140,759
_2837
EX28DRAFT_2838: EX28DRAFT_2838 - Membrane proteins related to metalloendopeptidases, at 140,819 to 141,943
_2838
EX28DRAFT_2839: EX28DRAFT_2839 - protein-L-isoaspartate(D-aspartate) O-methyltransferase, at 142,060 to 142,686
_2839
EX28DRAFT_2840: EX28DRAFT_2840 - 3'-nucleotidase (EC 3.1.3.6)/5'-nucleotidase (EC 3.1.3.5)/exopolyphosphatase (EC 3.6.1.11), at 142,680 to 143,441
_2840
Position (kb)
140
141
142 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 140.102 kb on + strand, within EX28DRAFT_2837 at 140.102 kb on + strand, within EX28DRAFT_2837 at 140.103 kb on - strand, within EX28DRAFT_2837 at 140.126 kb on + strand, within EX28DRAFT_2837 at 140.126 kb on + strand, within EX28DRAFT_2837 at 140.199 kb on - strand, within EX28DRAFT_2837 at 140.286 kb on - strand, within EX28DRAFT_2837 at 140.286 kb on - strand, within EX28DRAFT_2837 at 140.286 kb on - strand, within EX28DRAFT_2837 at 140.385 kb on + strand, within EX28DRAFT_2837 at 140.385 kb on + strand, within EX28DRAFT_2837 at 140.386 kb on - strand, within EX28DRAFT_2837 at 140.527 kb on + strand, within EX28DRAFT_2837 at 140.579 kb on - strand, within EX28DRAFT_2837 at 140.579 kb on - strand, within EX28DRAFT_2837 at 140.598 kb on + strand, within EX28DRAFT_2837 at 140.667 kb on + strand at 140.668 kb on - strand at 140.671 kb on - strand at 140.727 kb on + strand at 140.760 kb on - strand at 140.760 kb on - strand at 140.890 kb on + strand at 140.939 kb on - strand, within EX28DRAFT_2838 at 140.983 kb on - strand, within EX28DRAFT_2838 at 140.995 kb on - strand, within EX28DRAFT_2838 at 141.005 kb on - strand, within EX28DRAFT_2838 at 141.017 kb on - strand, within EX28DRAFT_2838 at 141.019 kb on + strand, within EX28DRAFT_2838 at 141.020 kb on - strand, within EX28DRAFT_2838 at 141.121 kb on + strand, within EX28DRAFT_2838 at 141.208 kb on + strand, within EX28DRAFT_2838 at 141.209 kb on - strand, within EX28DRAFT_2838 at 141.209 kb on - strand, within EX28DRAFT_2838 at 141.283 kb on + strand, within EX28DRAFT_2838 at 141.287 kb on - strand, within EX28DRAFT_2838 at 141.305 kb on - strand, within EX28DRAFT_2838 at 141.337 kb on + strand, within EX28DRAFT_2838 at 141.386 kb on - strand, within EX28DRAFT_2838 at 141.440 kb on - strand, within EX28DRAFT_2838 at 141.561 kb on + strand, within EX28DRAFT_2838 at 141.561 kb on + strand, within EX28DRAFT_2838 at 141.562 kb on - strand, within EX28DRAFT_2838 at 141.590 kb on - strand, within EX28DRAFT_2838 at 141.591 kb on + strand, within EX28DRAFT_2838 at 141.591 kb on + strand, within EX28DRAFT_2838 at 141.591 kb on + strand, within EX28DRAFT_2838 at 141.592 kb on - strand, within EX28DRAFT_2838 at 141.609 kb on + strand, within EX28DRAFT_2838 at 141.645 kb on + strand, within EX28DRAFT_2838 at 141.856 kb on + strand at 141.857 kb on - strand at 141.857 kb on - strand at 141.866 kb on - strand at 141.945 kb on + strand at 141.968 kb on - strand at 141.986 kb on - strand at 142.020 kb on - strand at 142.038 kb on + strand at 142.039 kb on - strand at 142.120 kb on - strand at 142.317 kb on + strand, within EX28DRAFT_2839 at 142.360 kb on + strand, within EX28DRAFT_2839 at 142.361 kb on - strand, within EX28DRAFT_2839 at 142.387 kb on + strand, within EX28DRAFT_2839 at 142.387 kb on + strand, within EX28DRAFT_2839 at 142.388 kb on - strand, within EX28DRAFT_2839 at 142.388 kb on - strand, within EX28DRAFT_2839 at 142.388 kb on - strand, within EX28DRAFT_2839 at 142.502 kb on + strand, within EX28DRAFT_2839 at 142.502 kb on + strand, within EX28DRAFT_2839 at 142.502 kb on + strand, within EX28DRAFT_2839 at 142.503 kb on - strand, within EX28DRAFT_2839 at 142.503 kb on - strand, within EX28DRAFT_2839 at 142.503 kb on - strand, within EX28DRAFT_2839 at 142.503 kb on - strand, within EX28DRAFT_2839 at 142.503 kb on - strand, within EX28DRAFT_2839 at 142.544 kb on - strand, within EX28DRAFT_2839 at 142.580 kb on - strand, within EX28DRAFT_2839 at 142.740 kb on - strand at 142.742 kb on - strand at 142.781 kb on - strand, within EX28DRAFT_2840 at 142.830 kb on - strand, within EX28DRAFT_2840
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM remove 140,102 + EX28DRAFT_2837 0.34 +0.7 140,102 + EX28DRAFT_2837 0.34 +0.2 140,103 - EX28DRAFT_2837 0.34 +1.5 140,126 + EX28DRAFT_2837 0.36 +0.6 140,126 + EX28DRAFT_2837 0.36 +1.3 140,199 - EX28DRAFT_2837 0.44 +2.0 140,286 - EX28DRAFT_2837 0.52 +0.9 140,286 - EX28DRAFT_2837 0.52 +2.4 140,286 - EX28DRAFT_2837 0.52 +2.2 140,385 + EX28DRAFT_2837 0.62 +2.4 140,385 + EX28DRAFT_2837 0.62 +2.3 140,386 - EX28DRAFT_2837 0.62 +1.8 140,527 + EX28DRAFT_2837 0.77 +1.9 140,579 - EX28DRAFT_2837 0.82 +0.7 140,579 - EX28DRAFT_2837 0.82 +1.9 140,598 + EX28DRAFT_2837 0.84 +0.8 140,667 + +2.8 140,668 - +0.1 140,671 - +2.5 140,727 + +0.8 140,760 - +2.3 140,760 - +2.7 140,890 + +0.4 140,939 - EX28DRAFT_2838 0.11 +0.6 140,983 - EX28DRAFT_2838 0.15 +1.4 140,995 - EX28DRAFT_2838 0.16 +1.4 141,005 - EX28DRAFT_2838 0.17 +1.1 141,017 - EX28DRAFT_2838 0.18 +0.1 141,019 + EX28DRAFT_2838 0.18 +1.1 141,020 - EX28DRAFT_2838 0.18 +1.2 141,121 + EX28DRAFT_2838 0.27 +1.2 141,208 + EX28DRAFT_2838 0.35 +1.6 141,209 - EX28DRAFT_2838 0.35 +1.9 141,209 - EX28DRAFT_2838 0.35 -0.0 141,283 + EX28DRAFT_2838 0.41 +1.7 141,287 - EX28DRAFT_2838 0.42 +0.8 141,305 - EX28DRAFT_2838 0.43 +1.0 141,337 + EX28DRAFT_2838 0.46 +1.7 141,386 - EX28DRAFT_2838 0.50 -0.0 141,440 - EX28DRAFT_2838 0.55 -0.3 141,561 + EX28DRAFT_2838 0.66 -0.0 141,561 + EX28DRAFT_2838 0.66 -0.3 141,562 - EX28DRAFT_2838 0.66 +0.0 141,590 - EX28DRAFT_2838 0.69 -0.3 141,591 + EX28DRAFT_2838 0.69 +0.5 141,591 + EX28DRAFT_2838 0.69 -0.0 141,591 + EX28DRAFT_2838 0.69 -1.6 141,592 - EX28DRAFT_2838 0.69 +0.1 141,609 + EX28DRAFT_2838 0.70 -1.5 141,645 + EX28DRAFT_2838 0.73 -0.7 141,856 + +0.1 141,857 - +0.3 141,857 - -0.3 141,866 - -0.2 141,945 + +0.5 141,968 - -0.3 141,986 - +0.2 142,020 - -0.9 142,038 + -0.4 142,039 - -0.9 142,120 - -3.9 142,317 + EX28DRAFT_2839 0.41 -1.4 142,360 + EX28DRAFT_2839 0.48 -0.5 142,361 - EX28DRAFT_2839 0.48 -0.0 142,387 + EX28DRAFT_2839 0.52 -0.0 142,387 + EX28DRAFT_2839 0.52 -0.6 142,388 - EX28DRAFT_2839 0.52 -0.8 142,388 - EX28DRAFT_2839 0.52 -2.1 142,388 - EX28DRAFT_2839 0.52 -0.2 142,502 + EX28DRAFT_2839 0.70 -1.2 142,502 + EX28DRAFT_2839 0.70 +0.1 142,502 + EX28DRAFT_2839 0.70 -1.2 142,503 - EX28DRAFT_2839 0.71 -0.4 142,503 - EX28DRAFT_2839 0.71 -0.5 142,503 - EX28DRAFT_2839 0.71 -0.6 142,503 - EX28DRAFT_2839 0.71 -0.2 142,503 - EX28DRAFT_2839 0.71 -0.9 142,544 - EX28DRAFT_2839 0.77 -1.2 142,580 - EX28DRAFT_2839 0.83 -1.9 142,740 - -1.0 142,742 - -2.4 142,781 - EX28DRAFT_2840 0.13 -0.3 142,830 - EX28DRAFT_2840 0.20 -0.4
Or see this region's nucleotide sequence