Experiment: MS_media with m-arsenite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2717 and EX28DRAFT_2718 are separated by 13 nucleotides EX28DRAFT_2718 and EX28DRAFT_2719 are separated by 85 nucleotides EX28DRAFT_2719 and EX28DRAFT_2720 are separated by 138 nucleotides EX28DRAFT_2720 and EX28DRAFT_2721 are separated by 103 nucleotides EX28DRAFT_2721 and EX28DRAFT_2722 overlap by 11 nucleotides
EX28DRAFT_2717: EX28DRAFT_2717 - hypothetical protein, at 22,265 to 22,381
_2717
EX28DRAFT_2718: EX28DRAFT_2718 - Predicted sugar kinase, at 22,395 to 23,273
_2718
EX28DRAFT_2719: EX28DRAFT_2719 - DNA repair protein RecN, at 23,359 to 25,020
_2719
EX28DRAFT_2720: EX28DRAFT_2720 - Small protein A (tmRNA-binding), at 25,159 to 25,497
_2720
EX28DRAFT_2721: EX28DRAFT_2721 - Uncharacterized protein conserved in bacteria, at 25,601 to 25,888
_2721
EX28DRAFT_2722: EX28DRAFT_2722 - Oligoketide cyclase/lipid transport protein, at 25,878 to 26,315
_2722
Position (kb) 23
24
25
26 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 23.297 kb on - strand at 23.300 kb on + strand at 23.301 kb on - strand at 23.358 kb on + strand at 23.358 kb on + strand at 23.359 kb on - strand at 23.461 kb on - strand at 23.461 kb on - strand at 23.461 kb on - strand at 23.461 kb on - strand at 23.517 kb on + strand at 23.676 kb on + strand, within EX28DRAFT_2719 at 23.677 kb on - strand, within EX28DRAFT_2719 at 23.823 kb on + strand, within EX28DRAFT_2719 at 23.941 kb on - strand, within EX28DRAFT_2719 at 23.998 kb on + strand, within EX28DRAFT_2719 at 23.999 kb on - strand, within EX28DRAFT_2719 at 23.999 kb on - strand, within EX28DRAFT_2719 at 24.097 kb on + strand, within EX28DRAFT_2719 at 24.097 kb on + strand, within EX28DRAFT_2719 at 24.097 kb on + strand, within EX28DRAFT_2719 at 24.097 kb on + strand, within EX28DRAFT_2719 at 24.098 kb on - strand, within EX28DRAFT_2719 at 24.098 kb on - strand, within EX28DRAFT_2719 at 24.331 kb on + strand, within EX28DRAFT_2719 at 24.332 kb on - strand, within EX28DRAFT_2719 at 24.482 kb on + strand, within EX28DRAFT_2719 at 24.834 kb on - strand, within EX28DRAFT_2719 at 25.045 kb on - strand at 25.094 kb on + strand at 25.095 kb on - strand at 25.108 kb on + strand at 25.109 kb on - strand at 25.111 kb on + strand at 25.111 kb on + strand at 25.111 kb on + strand at 25.111 kb on + strand at 25.111 kb on + strand at 25.112 kb on - strand at 25.115 kb on + strand at 25.116 kb on - strand at 25.116 kb on - strand at 25.267 kb on + strand, within EX28DRAFT_2720 at 25.268 kb on - strand, within EX28DRAFT_2720 at 25.268 kb on - strand, within EX28DRAFT_2720 at 25.268 kb on - strand, within EX28DRAFT_2720 at 25.268 kb on - strand, within EX28DRAFT_2720 at 25.330 kb on - strand, within EX28DRAFT_2720 at 25.381 kb on + strand, within EX28DRAFT_2720 at 25.381 kb on + strand, within EX28DRAFT_2720 at 25.381 kb on + strand, within EX28DRAFT_2720 at 25.381 kb on + strand, within EX28DRAFT_2720 at 25.382 kb on - strand, within EX28DRAFT_2720 at 25.382 kb on - strand, within EX28DRAFT_2720 at 25.382 kb on - strand, within EX28DRAFT_2720 at 25.382 kb on - strand, within EX28DRAFT_2720 at 25.385 kb on + strand, within EX28DRAFT_2720 at 25.385 kb on + strand, within EX28DRAFT_2720 at 25.386 kb on - strand, within EX28DRAFT_2720 at 25.495 kb on + strand at 25.496 kb on - strand at 25.602 kb on + strand at 25.602 kb on + strand at 25.603 kb on - strand at 25.606 kb on + strand at 25.837 kb on - strand, within EX28DRAFT_2721 at 25.858 kb on - strand, within EX28DRAFT_2721 at 25.863 kb on - strand at 25.863 kb on - strand at 25.868 kb on + strand at 25.868 kb on + strand at 25.891 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM remove 23,297 - -2.5 23,300 + +1.0 23,301 - +0.3 23,358 + -1.0 23,358 + -1.0 23,359 - -0.7 23,461 - -0.9 23,461 - +0.0 23,461 - -0.1 23,461 - -0.2 23,517 + -0.0 23,676 + EX28DRAFT_2719 0.19 -0.1 23,677 - EX28DRAFT_2719 0.19 -2.4 23,823 + EX28DRAFT_2719 0.28 -0.4 23,941 - EX28DRAFT_2719 0.35 -3.2 23,998 + EX28DRAFT_2719 0.38 -0.3 23,999 - EX28DRAFT_2719 0.39 -0.4 23,999 - EX28DRAFT_2719 0.39 +1.9 24,097 + EX28DRAFT_2719 0.44 -1.2 24,097 + EX28DRAFT_2719 0.44 -0.5 24,097 + EX28DRAFT_2719 0.44 -0.6 24,097 + EX28DRAFT_2719 0.44 +0.3 24,098 - EX28DRAFT_2719 0.44 -1.5 24,098 - EX28DRAFT_2719 0.44 -0.6 24,331 + EX28DRAFT_2719 0.58 -1.1 24,332 - EX28DRAFT_2719 0.59 +0.4 24,482 + EX28DRAFT_2719 0.68 -0.5 24,834 - EX28DRAFT_2719 0.89 -0.0 25,045 - +0.6 25,094 + -0.8 25,095 - -0.6 25,108 + +0.1 25,109 - -0.7 25,111 + +0.4 25,111 + -1.0 25,111 + -1.5 25,111 + -0.4 25,111 + +0.4 25,112 - -1.1 25,115 + -1.9 25,116 - -0.9 25,116 - -1.4 25,267 + EX28DRAFT_2720 0.32 +0.2 25,268 - EX28DRAFT_2720 0.32 -0.5 25,268 - EX28DRAFT_2720 0.32 -0.2 25,268 - EX28DRAFT_2720 0.32 -1.4 25,268 - EX28DRAFT_2720 0.32 -0.9 25,330 - EX28DRAFT_2720 0.50 -1.5 25,381 + EX28DRAFT_2720 0.65 -2.6 25,381 + EX28DRAFT_2720 0.65 -1.4 25,381 + EX28DRAFT_2720 0.65 -0.2 25,381 + EX28DRAFT_2720 0.65 -1.2 25,382 - EX28DRAFT_2720 0.66 -1.6 25,382 - EX28DRAFT_2720 0.66 +0.2 25,382 - EX28DRAFT_2720 0.66 -0.9 25,382 - EX28DRAFT_2720 0.66 -0.4 25,385 + EX28DRAFT_2720 0.67 -0.2 25,385 + EX28DRAFT_2720 0.67 -0.9 25,386 - EX28DRAFT_2720 0.67 -1.5 25,495 + -0.6 25,496 - -0.2 25,602 + -0.7 25,602 + -3.8 25,603 - +0.3 25,606 + -0.3 25,837 - EX28DRAFT_2721 0.82 -2.3 25,858 - EX28DRAFT_2721 0.89 -1.5 25,863 - -1.1 25,863 - -1.5 25,868 + -2.8 25,868 + -1.8 25,891 + -1.1
Or see this region's nucleotide sequence