Experiment: MS_media with m-arsenite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2696 and EX28DRAFT_2697 are separated by 274 nucleotides EX28DRAFT_2697 and EX28DRAFT_2698 are separated by 110 nucleotides EX28DRAFT_2698 and EX28DRAFT_2699 are separated by 30 nucleotides EX28DRAFT_2699 and EX28DRAFT_2700 overlap by 4 nucleotides EX28DRAFT_2700 and EX28DRAFT_2701 are separated by 60 nucleotides
EX28DRAFT_2696: EX28DRAFT_2696 - Beta-barrel assembly machine subunit BamD, at 5,041 to 5,778
_2696
EX28DRAFT_2697: EX28DRAFT_2697 - ribosomal subunit interface protein, at 6,053 to 6,394
_2697
EX28DRAFT_2698: EX28DRAFT_2698 - RNA, at 6,505 to 6,630
_2698
EX28DRAFT_2699: EX28DRAFT_2699 - chorismate mutase (EC 5.4.99.5), at 6,661 to 7,821
_2699
EX28DRAFT_2700: EX28DRAFT_2700 - Gluconolactonase, at 7,818 to 8,690
_2700
EX28DRAFT_2701: EX28DRAFT_2701 - chorismate mutase (EC 5.4.99.5), at 8,751 to 9,872
_2701
Position (kb)
6
7
8 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 5.839 kb on - strand at 5.883 kb on - strand at 6.002 kb on + strand at 6.003 kb on - strand at 6.046 kb on - strand at 6.212 kb on - strand, within EX28DRAFT_2697 at 6.212 kb on - strand, within EX28DRAFT_2697 at 6.212 kb on - strand, within EX28DRAFT_2697 at 6.212 kb on - strand, within EX28DRAFT_2697 at 6.217 kb on + strand, within EX28DRAFT_2697 at 6.217 kb on + strand, within EX28DRAFT_2697 at 6.260 kb on - strand, within EX28DRAFT_2697 at 6.263 kb on + strand, within EX28DRAFT_2697 at 6.264 kb on - strand, within EX28DRAFT_2697 at 6.392 kb on + strand at 6.392 kb on + strand at 6.393 kb on - strand at 6.393 kb on - strand at 6.629 kb on - strand at 6.640 kb on + strand at 6.660 kb on + strand at 6.660 kb on + strand at 6.660 kb on + strand at 6.661 kb on - strand at 6.661 kb on - strand at 6.874 kb on + strand, within EX28DRAFT_2699 at 6.875 kb on - strand, within EX28DRAFT_2699 at 6.952 kb on - strand, within EX28DRAFT_2699 at 7.004 kb on - strand, within EX28DRAFT_2699 at 7.027 kb on + strand, within EX28DRAFT_2699 at 7.028 kb on - strand, within EX28DRAFT_2699 at 7.053 kb on + strand, within EX28DRAFT_2699 at 7.168 kb on + strand, within EX28DRAFT_2699 at 7.169 kb on - strand, within EX28DRAFT_2699 at 7.441 kb on + strand, within EX28DRAFT_2699 at 7.455 kb on - strand, within EX28DRAFT_2699 at 7.687 kb on + strand, within EX28DRAFT_2699 at 7.687 kb on + strand, within EX28DRAFT_2699 at 7.688 kb on - strand, within EX28DRAFT_2699 at 7.688 kb on - strand, within EX28DRAFT_2699 at 7.820 kb on - strand at 7.894 kb on + strand at 7.919 kb on + strand, within EX28DRAFT_2700 at 7.940 kb on + strand, within EX28DRAFT_2700 at 7.941 kb on - strand, within EX28DRAFT_2700 at 8.327 kb on - strand, within EX28DRAFT_2700 at 8.391 kb on - strand, within EX28DRAFT_2700 at 8.475 kb on - strand, within EX28DRAFT_2700 at 8.608 kb on + strand at 8.663 kb on - strand at 8.663 kb on - strand at 8.736 kb on - strand at 8.739 kb on + strand at 8.739 kb on + strand at 8.739 kb on + strand at 8.739 kb on + strand at 8.739 kb on + strand at 8.740 kb on - strand at 8.740 kb on - strand at 8.740 kb on - strand at 8.740 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM remove 5,839 - -0.1 5,883 - +0.4 6,002 + -0.7 6,003 - +0.4 6,046 - -0.0 6,212 - EX28DRAFT_2697 0.46 -0.2 6,212 - EX28DRAFT_2697 0.46 -0.4 6,212 - EX28DRAFT_2697 0.46 +1.8 6,212 - EX28DRAFT_2697 0.46 +0.2 6,217 + EX28DRAFT_2697 0.48 +0.3 6,217 + EX28DRAFT_2697 0.48 +0.6 6,260 - EX28DRAFT_2697 0.61 +1.5 6,263 + EX28DRAFT_2697 0.61 +0.8 6,264 - EX28DRAFT_2697 0.62 -0.3 6,392 + +0.1 6,392 + +0.8 6,393 - +1.8 6,393 - +0.6 6,629 - -2.4 6,640 + +0.0 6,660 + -3.9 6,660 + -3.1 6,660 + -1.9 6,661 - -3.9 6,661 - -4.2 6,874 + EX28DRAFT_2699 0.18 -2.8 6,875 - EX28DRAFT_2699 0.18 -4.4 6,952 - EX28DRAFT_2699 0.25 +1.1 7,004 - EX28DRAFT_2699 0.30 +0.5 7,027 + EX28DRAFT_2699 0.32 -1.4 7,028 - EX28DRAFT_2699 0.32 -3.9 7,053 + EX28DRAFT_2699 0.34 -3.3 7,168 + EX28DRAFT_2699 0.44 -0.4 7,169 - EX28DRAFT_2699 0.44 -2.1 7,441 + EX28DRAFT_2699 0.67 -2.7 7,455 - EX28DRAFT_2699 0.68 -0.9 7,687 + EX28DRAFT_2699 0.88 -0.9 7,687 + EX28DRAFT_2699 0.88 -2.3 7,688 - EX28DRAFT_2699 0.88 -1.6 7,688 - EX28DRAFT_2699 0.88 -1.3 7,820 - -0.9 7,894 + +0.2 7,919 + EX28DRAFT_2700 0.12 +0.0 7,940 + EX28DRAFT_2700 0.14 -0.1 7,941 - EX28DRAFT_2700 0.14 +0.3 8,327 - EX28DRAFT_2700 0.58 -1.4 8,391 - EX28DRAFT_2700 0.66 +0.1 8,475 - EX28DRAFT_2700 0.75 +0.1 8,608 + +0.0 8,663 - -0.4 8,663 - +0.9 8,736 - +0.1 8,739 + +0.7 8,739 + -0.0 8,739 + -0.1 8,739 + +0.3 8,739 + -0.3 8,740 - +0.0 8,740 - -3.8 8,740 - +1.2 8,740 - -0.4
Or see this region's nucleotide sequence