Experiment: MS_media with m-arsenite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2679 and EX28DRAFT_2680 are separated by 55 nucleotides EX28DRAFT_2680 and EX28DRAFT_2681 overlap by 4 nucleotides EX28DRAFT_2681 and EX28DRAFT_2682 are separated by 32 nucleotides EX28DRAFT_2682 and EX28DRAFT_2683 are separated by 68 nucleotides
EX28DRAFT_2679: EX28DRAFT_2679 - DNA polymerase III, epsilon subunit, Proteobacterial, at 2,716,581 to 2,717,321
_2679
EX28DRAFT_2680: EX28DRAFT_2680 - Ribonuclease HI, at 2,717,377 to 2,717,844
_2680
EX28DRAFT_2681: EX28DRAFT_2681 - Methyltransferase domain, at 2,717,841 to 2,718,560
_2681
EX28DRAFT_2682: EX28DRAFT_2682 - hydroxyacylglutathione hydrolase, at 2,718,593 to 2,719,351
_2682
EX28DRAFT_2683: EX28DRAFT_2683 - Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains), at 2,719,420 to 2,720,784
_2683
Position (kb)
2717
2718
2719 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2717.857 kb on + strand at 2717.927 kb on + strand, within EX28DRAFT_2681 at 2717.946 kb on - strand, within EX28DRAFT_2681 at 2717.958 kb on - strand, within EX28DRAFT_2681 at 2717.997 kb on + strand, within EX28DRAFT_2681 at 2717.997 kb on + strand, within EX28DRAFT_2681 at 2718.115 kb on + strand, within EX28DRAFT_2681 at 2718.115 kb on + strand, within EX28DRAFT_2681 at 2718.150 kb on - strand, within EX28DRAFT_2681 at 2718.189 kb on + strand, within EX28DRAFT_2681 at 2718.189 kb on + strand, within EX28DRAFT_2681 at 2718.189 kb on + strand, within EX28DRAFT_2681 at 2718.266 kb on + strand, within EX28DRAFT_2681 at 2718.266 kb on + strand, within EX28DRAFT_2681 at 2718.266 kb on + strand, within EX28DRAFT_2681 at 2718.266 kb on + strand, within EX28DRAFT_2681 at 2718.266 kb on + strand, within EX28DRAFT_2681 at 2718.266 kb on + strand, within EX28DRAFT_2681 at 2718.266 kb on + strand, within EX28DRAFT_2681 at 2718.267 kb on - strand, within EX28DRAFT_2681 at 2718.267 kb on - strand, within EX28DRAFT_2681 at 2718.267 kb on - strand, within EX28DRAFT_2681 at 2718.267 kb on - strand, within EX28DRAFT_2681 at 2718.267 kb on - strand, within EX28DRAFT_2681 at 2718.362 kb on + strand, within EX28DRAFT_2681 at 2718.378 kb on - strand, within EX28DRAFT_2681 at 2718.439 kb on + strand, within EX28DRAFT_2681 at 2718.487 kb on + strand, within EX28DRAFT_2681 at 2718.490 kb on + strand at 2718.490 kb on + strand at 2718.490 kb on + strand at 2718.490 kb on + strand at 2718.491 kb on - strand at 2718.543 kb on + strand at 2718.543 kb on + strand at 2718.581 kb on - strand at 2718.587 kb on + strand at 2718.587 kb on + strand at 2718.587 kb on + strand at 2718.588 kb on - strand at 2718.592 kb on + strand at 2718.593 kb on - strand at 2718.607 kb on + strand at 2718.629 kb on + strand at 2718.629 kb on + strand at 2718.629 kb on + strand at 2718.630 kb on - strand at 2718.630 kb on - strand at 2718.708 kb on + strand, within EX28DRAFT_2682 at 2718.838 kb on + strand, within EX28DRAFT_2682 at 2718.855 kb on + strand, within EX28DRAFT_2682 at 2718.956 kb on + strand, within EX28DRAFT_2682 at 2719.093 kb on + strand, within EX28DRAFT_2682 at 2719.093 kb on + strand, within EX28DRAFT_2682 at 2719.176 kb on + strand, within EX28DRAFT_2682 at 2719.400 kb on - strand at 2719.407 kb on + strand at 2719.461 kb on - strand at 2719.461 kb on - strand at 2719.461 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM remove 2,717,857 + -0.6 2,717,927 + EX28DRAFT_2681 0.12 +0.7 2,717,946 - EX28DRAFT_2681 0.15 +0.7 2,717,958 - EX28DRAFT_2681 0.16 +0.8 2,717,997 + EX28DRAFT_2681 0.22 +0.8 2,717,997 + EX28DRAFT_2681 0.22 +0.5 2,718,115 + EX28DRAFT_2681 0.38 +0.1 2,718,115 + EX28DRAFT_2681 0.38 +0.6 2,718,150 - EX28DRAFT_2681 0.43 +1.2 2,718,189 + EX28DRAFT_2681 0.48 +0.9 2,718,189 + EX28DRAFT_2681 0.48 -0.2 2,718,189 + EX28DRAFT_2681 0.48 +0.8 2,718,266 + EX28DRAFT_2681 0.59 +0.5 2,718,266 + EX28DRAFT_2681 0.59 +0.6 2,718,266 + EX28DRAFT_2681 0.59 +0.6 2,718,266 + EX28DRAFT_2681 0.59 +0.7 2,718,266 + EX28DRAFT_2681 0.59 +1.0 2,718,266 + EX28DRAFT_2681 0.59 +1.6 2,718,266 + EX28DRAFT_2681 0.59 +0.3 2,718,267 - EX28DRAFT_2681 0.59 -4.5 2,718,267 - EX28DRAFT_2681 0.59 +1.0 2,718,267 - EX28DRAFT_2681 0.59 +0.8 2,718,267 - EX28DRAFT_2681 0.59 +0.8 2,718,267 - EX28DRAFT_2681 0.59 -0.1 2,718,362 + EX28DRAFT_2681 0.72 +0.1 2,718,378 - EX28DRAFT_2681 0.75 +0.9 2,718,439 + EX28DRAFT_2681 0.83 +0.4 2,718,487 + EX28DRAFT_2681 0.90 +0.6 2,718,490 + +0.2 2,718,490 + +0.4 2,718,490 + +1.4 2,718,490 + -0.2 2,718,491 - +0.4 2,718,543 + +0.2 2,718,543 + +0.4 2,718,581 - +0.3 2,718,587 + +0.5 2,718,587 + +0.3 2,718,587 + -0.1 2,718,588 - +0.6 2,718,592 + +1.0 2,718,593 - +1.0 2,718,607 + +0.1 2,718,629 + +0.1 2,718,629 + -0.2 2,718,629 + +1.5 2,718,630 - +0.5 2,718,630 - -0.4 2,718,708 + EX28DRAFT_2682 0.15 -0.0 2,718,838 + EX28DRAFT_2682 0.32 +0.4 2,718,855 + EX28DRAFT_2682 0.35 +0.7 2,718,956 + EX28DRAFT_2682 0.48 +0.1 2,719,093 + EX28DRAFT_2682 0.66 +0.1 2,719,093 + EX28DRAFT_2682 0.66 -0.6 2,719,176 + EX28DRAFT_2682 0.77 +0.5 2,719,400 - +0.4 2,719,407 + +1.4 2,719,461 - +0.2 2,719,461 - -0.6 2,719,461 - +0.4
Or see this region's nucleotide sequence