Experiment: MS_media with m-arsenite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2544 and EX28DRAFT_2545 are separated by 32 nucleotides EX28DRAFT_2545 and EX28DRAFT_2546 are separated by 121 nucleotides EX28DRAFT_2546 and EX28DRAFT_2547 overlap by 38 nucleotides EX28DRAFT_2547 and EX28DRAFT_2548 are separated by 67 nucleotides
EX28DRAFT_2544: EX28DRAFT_2544 - Arabinose efflux permease, at 2,576,565 to 2,577,926
_2544
EX28DRAFT_2545: EX28DRAFT_2545 - Uncharacterized protein conserved in bacteria, at 2,577,959 to 2,578,450
_2545
EX28DRAFT_2546: EX28DRAFT_2546 - 2-dehydropantoate 2-reductase, at 2,578,572 to 2,579,483
_2546
EX28DRAFT_2547: EX28DRAFT_2547 - DJ-1 family protein, at 2,579,446 to 2,580,036
_2547
EX28DRAFT_2548: EX28DRAFT_2548 - tRNA sulfurtransferase ThiI/thiazole biosynthesis domain, at 2,580,104 to 2,581,552
_2548
Position (kb)
2578
2579
2580 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2577.591 kb on + strand, within EX28DRAFT_2544 at 2577.592 kb on - strand, within EX28DRAFT_2544 at 2577.741 kb on + strand, within EX28DRAFT_2544 at 2577.797 kb on + strand at 2577.798 kb on - strand at 2577.874 kb on + strand at 2577.874 kb on + strand at 2578.093 kb on + strand, within EX28DRAFT_2545 at 2578.093 kb on + strand, within EX28DRAFT_2545 at 2578.109 kb on - strand, within EX28DRAFT_2545 at 2578.109 kb on - strand, within EX28DRAFT_2545 at 2578.111 kb on + strand, within EX28DRAFT_2545 at 2578.111 kb on + strand, within EX28DRAFT_2545 at 2578.111 kb on + strand, within EX28DRAFT_2545 at 2578.112 kb on - strand, within EX28DRAFT_2545 at 2578.112 kb on - strand, within EX28DRAFT_2545 at 2578.307 kb on - strand, within EX28DRAFT_2545 at 2578.484 kb on + strand at 2578.519 kb on + strand at 2578.525 kb on + strand at 2578.526 kb on - strand at 2578.527 kb on + strand at 2578.528 kb on - strand at 2578.531 kb on + strand at 2578.531 kb on + strand at 2578.532 kb on - strand at 2578.678 kb on - strand, within EX28DRAFT_2546 at 2578.772 kb on - strand, within EX28DRAFT_2546 at 2579.017 kb on - strand, within EX28DRAFT_2546 at 2579.234 kb on + strand, within EX28DRAFT_2546 at 2579.235 kb on - strand, within EX28DRAFT_2546 at 2579.259 kb on + strand, within EX28DRAFT_2546 at 2579.260 kb on - strand, within EX28DRAFT_2546 at 2579.295 kb on - strand, within EX28DRAFT_2546 at 2579.432 kb on + strand at 2579.446 kb on - strand at 2579.446 kb on - strand at 2579.506 kb on - strand, within EX28DRAFT_2547 at 2579.980 kb on + strand at 2580.019 kb on + strand at 2580.025 kb on + strand at 2580.025 kb on + strand at 2580.025 kb on + strand at 2580.026 kb on - strand at 2580.028 kb on + strand at 2580.034 kb on + strand at 2580.034 kb on + strand at 2580.034 kb on + strand at 2580.034 kb on + strand at 2580.035 kb on - strand at 2580.035 kb on - strand at 2580.035 kb on - strand at 2580.106 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM remove 2,577,591 + EX28DRAFT_2544 0.75 +0.1 2,577,592 - EX28DRAFT_2544 0.75 +1.2 2,577,741 + EX28DRAFT_2544 0.86 +0.3 2,577,797 + -0.6 2,577,798 - +0.9 2,577,874 + -0.2 2,577,874 + +3.0 2,578,093 + EX28DRAFT_2545 0.27 +0.5 2,578,093 + EX28DRAFT_2545 0.27 -0.7 2,578,109 - EX28DRAFT_2545 0.30 -0.7 2,578,109 - EX28DRAFT_2545 0.30 +0.1 2,578,111 + EX28DRAFT_2545 0.31 -0.4 2,578,111 + EX28DRAFT_2545 0.31 +0.9 2,578,111 + EX28DRAFT_2545 0.31 -0.0 2,578,112 - EX28DRAFT_2545 0.31 -0.2 2,578,112 - EX28DRAFT_2545 0.31 +0.1 2,578,307 - EX28DRAFT_2545 0.71 -0.6 2,578,484 + -0.1 2,578,519 + -0.1 2,578,525 + -0.8 2,578,526 - -0.1 2,578,527 + +0.0 2,578,528 - +0.3 2,578,531 + +0.1 2,578,531 + +0.1 2,578,532 - +0.3 2,578,678 - EX28DRAFT_2546 0.12 -2.4 2,578,772 - EX28DRAFT_2546 0.22 -2.2 2,579,017 - EX28DRAFT_2546 0.49 -2.4 2,579,234 + EX28DRAFT_2546 0.73 -1.8 2,579,235 - EX28DRAFT_2546 0.73 -2.8 2,579,259 + EX28DRAFT_2546 0.75 -2.7 2,579,260 - EX28DRAFT_2546 0.75 -2.0 2,579,295 - EX28DRAFT_2546 0.79 -1.0 2,579,432 + +1.0 2,579,446 - -0.0 2,579,446 - +0.7 2,579,506 - EX28DRAFT_2547 0.10 +0.3 2,579,980 + -0.2 2,580,019 + -0.2 2,580,025 + +0.3 2,580,025 + +0.2 2,580,025 + +1.2 2,580,026 - +0.5 2,580,028 + +0.1 2,580,034 + -0.3 2,580,034 + -0.0 2,580,034 + +0.5 2,580,034 + +0.3 2,580,035 - -0.1 2,580,035 - +0.4 2,580,035 - -0.8 2,580,106 - +0.4
Or see this region's nucleotide sequence