Experiment: MS_media with m-arsenite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2342 and EX28DRAFT_2343 are separated by 94 nucleotides EX28DRAFT_2343 and EX28DRAFT_2344 overlap by 7 nucleotides EX28DRAFT_2344 and EX28DRAFT_2345 are separated by 115 nucleotides EX28DRAFT_2345 and EX28DRAFT_2346 are separated by 43 nucleotides EX28DRAFT_2346 and EX28DRAFT_2347 are separated by 237 nucleotides
EX28DRAFT_2342: EX28DRAFT_2342 - c4-dicarboxylate anaerobic carrier family protein, at 2,364,327 to 2,365,694
_2342
EX28DRAFT_2343: EX28DRAFT_2343 - Ribonuclease I, at 2,365,789 to 2,366,598
_2343
EX28DRAFT_2344: EX28DRAFT_2344 - Conserved protein/domain typically associated with flavoprotein oxygenases, DIM6/NTAB family, at 2,366,592 to 2,367,209
_2344
EX28DRAFT_2345: EX28DRAFT_2345 - RNA, at 2,367,325 to 2,367,433
_2345
EX28DRAFT_2346: EX28DRAFT_2346 - Transcription elongation factor, at 2,367,477 to 2,367,887
_2346
EX28DRAFT_2347: EX28DRAFT_2347 - Threonine dehydrogenase and related Zn-dependent dehydrogenases, at 2,368,125 to 2,369,363
_2347
Position (kb)
2366
2367
2368 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2365.636 kb on + strand at 2365.636 kb on + strand at 2365.636 kb on + strand at 2365.637 kb on - strand at 2365.637 kb on - strand at 2365.692 kb on + strand at 2365.697 kb on + strand at 2365.703 kb on - strand at 2365.703 kb on - strand at 2365.747 kb on - strand at 2365.759 kb on + strand at 2365.788 kb on + strand at 2365.788 kb on + strand at 2365.789 kb on - strand at 2365.883 kb on - strand, within EX28DRAFT_2343 at 2365.900 kb on + strand, within EX28DRAFT_2343 at 2365.901 kb on - strand, within EX28DRAFT_2343 at 2366.002 kb on - strand, within EX28DRAFT_2343 at 2366.002 kb on - strand, within EX28DRAFT_2343 at 2366.030 kb on + strand, within EX28DRAFT_2343 at 2366.031 kb on - strand, within EX28DRAFT_2343 at 2366.243 kb on - strand, within EX28DRAFT_2343 at 2366.291 kb on - strand, within EX28DRAFT_2343 at 2366.291 kb on - strand, within EX28DRAFT_2343 at 2366.291 kb on - strand, within EX28DRAFT_2343 at 2366.468 kb on - strand, within EX28DRAFT_2343 at 2366.509 kb on - strand, within EX28DRAFT_2343 at 2366.509 kb on - strand, within EX28DRAFT_2343 at 2366.577 kb on - strand at 2366.617 kb on + strand at 2366.618 kb on + strand at 2366.651 kb on - strand at 2366.701 kb on + strand, within EX28DRAFT_2344 at 2366.701 kb on + strand, within EX28DRAFT_2344 at 2367.003 kb on - strand, within EX28DRAFT_2344 at 2367.005 kb on + strand, within EX28DRAFT_2344 at 2367.063 kb on - strand, within EX28DRAFT_2344 at 2367.063 kb on - strand, within EX28DRAFT_2344 at 2367.071 kb on + strand, within EX28DRAFT_2344 at 2367.072 kb on - strand, within EX28DRAFT_2344 at 2367.081 kb on - strand, within EX28DRAFT_2344 at 2367.081 kb on - strand, within EX28DRAFT_2344 at 2367.082 kb on + strand, within EX28DRAFT_2344 at 2367.200 kb on - strand at 2367.205 kb on + strand at 2367.205 kb on + strand at 2367.206 kb on - strand at 2367.206 kb on - strand at 2367.206 kb on - strand at 2367.209 kb on + strand at 2367.210 kb on - strand at 2367.329 kb on + strand at 2367.330 kb on - strand at 2367.472 kb on + strand at 2367.494 kb on + strand at 2367.494 kb on + strand at 2367.494 kb on + strand at 2367.494 kb on + strand at 2367.948 kb on + strand at 2367.968 kb on - strand at 2367.968 kb on - strand at 2368.122 kb on + strand at 2368.126 kb on + strand at 2368.127 kb on - strand at 2368.127 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM remove 2,365,636 + +0.4 2,365,636 + -2.9 2,365,636 + -0.1 2,365,637 - -0.9 2,365,637 - -2.0 2,365,692 + +0.3 2,365,697 + -0.5 2,365,703 - +0.1 2,365,703 - -0.5 2,365,747 - +0.3 2,365,759 + +0.4 2,365,788 + -0.7 2,365,788 + -0.6 2,365,789 - +0.2 2,365,883 - EX28DRAFT_2343 0.12 +0.3 2,365,900 + EX28DRAFT_2343 0.14 +1.1 2,365,901 - EX28DRAFT_2343 0.14 +0.1 2,366,002 - EX28DRAFT_2343 0.26 -0.1 2,366,002 - EX28DRAFT_2343 0.26 -1.0 2,366,030 + EX28DRAFT_2343 0.30 +0.7 2,366,031 - EX28DRAFT_2343 0.30 +2.0 2,366,243 - EX28DRAFT_2343 0.56 +0.3 2,366,291 - EX28DRAFT_2343 0.62 +0.1 2,366,291 - EX28DRAFT_2343 0.62 +0.0 2,366,291 - EX28DRAFT_2343 0.62 -0.1 2,366,468 - EX28DRAFT_2343 0.84 -0.6 2,366,509 - EX28DRAFT_2343 0.89 -0.9 2,366,509 - EX28DRAFT_2343 0.89 +0.2 2,366,577 - +0.0 2,366,617 + +1.4 2,366,618 + -0.5 2,366,651 - -0.3 2,366,701 + EX28DRAFT_2344 0.18 -0.2 2,366,701 + EX28DRAFT_2344 0.18 -0.1 2,367,003 - EX28DRAFT_2344 0.67 -0.4 2,367,005 + EX28DRAFT_2344 0.67 -0.7 2,367,063 - EX28DRAFT_2344 0.76 +0.1 2,367,063 - EX28DRAFT_2344 0.76 -1.5 2,367,071 + EX28DRAFT_2344 0.78 +0.7 2,367,072 - EX28DRAFT_2344 0.78 +0.1 2,367,081 - EX28DRAFT_2344 0.79 +0.3 2,367,081 - EX28DRAFT_2344 0.79 -1.0 2,367,082 + EX28DRAFT_2344 0.79 +0.8 2,367,200 - -0.7 2,367,205 + -0.2 2,367,205 + +0.8 2,367,206 - +0.7 2,367,206 - +0.1 2,367,206 - -0.0 2,367,209 + +0.6 2,367,210 - -0.0 2,367,329 + +0.4 2,367,330 - +1.2 2,367,472 + -0.0 2,367,494 + -0.6 2,367,494 + +0.2 2,367,494 + +0.6 2,367,494 + +1.2 2,367,948 + -0.1 2,367,968 - +0.3 2,367,968 - +0.3 2,368,122 + -0.7 2,368,126 + -0.1 2,368,127 - -0.2 2,368,127 - +0.7
Or see this region's nucleotide sequence