Experiment: MS_media with m-arsenite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2298 and EX28DRAFT_2299 are separated by 8 nucleotides EX28DRAFT_2299 and EX28DRAFT_2300 are separated by 45 nucleotides
EX28DRAFT_2298: EX28DRAFT_2298 - N-acetylglucosamine-6-phosphate deacetylase, at 2,327,627 to 2,328,775
_2298
EX28DRAFT_2299: EX28DRAFT_2299 - Transcriptional regulator/sugar kinase, at 2,328,784 to 2,330,004
_2299
EX28DRAFT_2300: EX28DRAFT_2300 - Predicted sugar phosphatases of the HAD superfamily, at 2,330,050 to 2,330,802
_2300
Position (kb)
2328
2329
2330
2331 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2327.800 kb on - strand, within EX28DRAFT_2298 at 2327.800 kb on - strand, within EX28DRAFT_2298 at 2327.909 kb on + strand, within EX28DRAFT_2298 at 2327.909 kb on + strand, within EX28DRAFT_2298 at 2327.909 kb on + strand, within EX28DRAFT_2298 at 2327.910 kb on - strand, within EX28DRAFT_2298 at 2327.992 kb on + strand, within EX28DRAFT_2298 at 2328.065 kb on + strand, within EX28DRAFT_2298 at 2328.066 kb on - strand, within EX28DRAFT_2298 at 2328.073 kb on + strand, within EX28DRAFT_2298 at 2328.098 kb on + strand, within EX28DRAFT_2298 at 2328.098 kb on + strand, within EX28DRAFT_2298 at 2328.099 kb on - strand, within EX28DRAFT_2298 at 2328.293 kb on + strand, within EX28DRAFT_2298 at 2328.293 kb on + strand, within EX28DRAFT_2298 at 2328.350 kb on + strand, within EX28DRAFT_2298 at 2328.362 kb on - strand, within EX28DRAFT_2298 at 2328.375 kb on + strand, within EX28DRAFT_2298 at 2328.375 kb on + strand, within EX28DRAFT_2298 at 2328.376 kb on - strand, within EX28DRAFT_2298 at 2328.376 kb on - strand, within EX28DRAFT_2298 at 2328.376 kb on - strand, within EX28DRAFT_2298 at 2328.504 kb on - strand, within EX28DRAFT_2298 at 2328.692 kb on - strand at 2328.773 kb on + strand at 2328.773 kb on + strand at 2328.777 kb on + strand at 2328.777 kb on + strand at 2328.783 kb on + strand at 2328.837 kb on + strand at 2328.837 kb on + strand at 2328.837 kb on + strand at 2328.837 kb on + strand at 2328.837 kb on + strand at 2329.104 kb on + strand, within EX28DRAFT_2299 at 2329.104 kb on + strand, within EX28DRAFT_2299 at 2329.127 kb on - strand, within EX28DRAFT_2299 at 2329.177 kb on - strand, within EX28DRAFT_2299 at 2329.192 kb on - strand, within EX28DRAFT_2299 at 2329.285 kb on - strand, within EX28DRAFT_2299 at 2329.342 kb on - strand, within EX28DRAFT_2299 at 2329.397 kb on - strand, within EX28DRAFT_2299 at 2329.397 kb on - strand, within EX28DRAFT_2299 at 2329.465 kb on - strand, within EX28DRAFT_2299 at 2329.467 kb on + strand, within EX28DRAFT_2299 at 2329.468 kb on - strand, within EX28DRAFT_2299 at 2329.674 kb on - strand, within EX28DRAFT_2299 at 2329.736 kb on + strand, within EX28DRAFT_2299 at 2329.737 kb on - strand, within EX28DRAFT_2299 at 2330.013 kb on + strand at 2330.013 kb on + strand at 2330.015 kb on + strand at 2330.015 kb on + strand at 2330.016 kb on - strand at 2330.016 kb on - strand at 2330.016 kb on - strand at 2330.016 kb on - strand at 2330.019 kb on - strand at 2330.059 kb on - strand at 2330.066 kb on + strand at 2330.066 kb on + strand at 2330.133 kb on - strand, within EX28DRAFT_2300 at 2330.179 kb on + strand, within EX28DRAFT_2300 at 2330.180 kb on - strand, within EX28DRAFT_2300 at 2330.254 kb on + strand, within EX28DRAFT_2300 at 2330.255 kb on - strand, within EX28DRAFT_2300 at 2330.255 kb on - strand, within EX28DRAFT_2300 at 2330.416 kb on - strand, within EX28DRAFT_2300 at 2330.443 kb on + strand, within EX28DRAFT_2300 at 2330.444 kb on - strand, within EX28DRAFT_2300 at 2330.837 kb on + strand at 2330.839 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM remove 2,327,800 - EX28DRAFT_2298 0.15 -0.5 2,327,800 - EX28DRAFT_2298 0.15 -0.9 2,327,909 + EX28DRAFT_2298 0.25 +0.4 2,327,909 + EX28DRAFT_2298 0.25 -0.3 2,327,909 + EX28DRAFT_2298 0.25 -0.8 2,327,910 - EX28DRAFT_2298 0.25 -2.1 2,327,992 + EX28DRAFT_2298 0.32 -0.9 2,328,065 + EX28DRAFT_2298 0.38 -0.3 2,328,066 - EX28DRAFT_2298 0.38 -1.3 2,328,073 + EX28DRAFT_2298 0.39 -0.3 2,328,098 + EX28DRAFT_2298 0.41 -0.4 2,328,098 + EX28DRAFT_2298 0.41 -1.3 2,328,099 - EX28DRAFT_2298 0.41 +0.1 2,328,293 + EX28DRAFT_2298 0.58 -0.7 2,328,293 + EX28DRAFT_2298 0.58 -0.4 2,328,350 + EX28DRAFT_2298 0.63 -1.2 2,328,362 - EX28DRAFT_2298 0.64 -1.8 2,328,375 + EX28DRAFT_2298 0.65 +0.7 2,328,375 + EX28DRAFT_2298 0.65 -0.7 2,328,376 - EX28DRAFT_2298 0.65 -2.2 2,328,376 - EX28DRAFT_2298 0.65 -1.6 2,328,376 - EX28DRAFT_2298 0.65 -1.1 2,328,504 - EX28DRAFT_2298 0.76 -1.5 2,328,692 - -2.6 2,328,773 + -0.8 2,328,773 + -1.8 2,328,777 + -0.6 2,328,777 + -1.2 2,328,783 + -1.0 2,328,837 + -3.2 2,328,837 + -1.4 2,328,837 + -1.7 2,328,837 + -1.8 2,328,837 + -3.5 2,329,104 + EX28DRAFT_2299 0.26 -1.7 2,329,104 + EX28DRAFT_2299 0.26 -0.7 2,329,127 - EX28DRAFT_2299 0.28 -1.4 2,329,177 - EX28DRAFT_2299 0.32 -2.7 2,329,192 - EX28DRAFT_2299 0.33 -1.7 2,329,285 - EX28DRAFT_2299 0.41 -2.0 2,329,342 - EX28DRAFT_2299 0.46 -1.0 2,329,397 - EX28DRAFT_2299 0.50 -2.7 2,329,397 - EX28DRAFT_2299 0.50 -3.8 2,329,465 - EX28DRAFT_2299 0.56 -2.7 2,329,467 + EX28DRAFT_2299 0.56 -1.7 2,329,468 - EX28DRAFT_2299 0.56 -1.6 2,329,674 - EX28DRAFT_2299 0.73 -2.6 2,329,736 + EX28DRAFT_2299 0.78 -1.3 2,329,737 - EX28DRAFT_2299 0.78 -2.4 2,330,013 + -0.0 2,330,013 + +0.2 2,330,015 + +0.1 2,330,015 + -0.3 2,330,016 - +0.2 2,330,016 - -0.4 2,330,016 - -0.8 2,330,016 - -1.8 2,330,019 - -0.7 2,330,059 - +0.0 2,330,066 + -0.0 2,330,066 + -0.1 2,330,133 - EX28DRAFT_2300 0.11 -0.3 2,330,179 + EX28DRAFT_2300 0.17 +0.6 2,330,180 - EX28DRAFT_2300 0.17 -0.5 2,330,254 + EX28DRAFT_2300 0.27 -3.2 2,330,255 - EX28DRAFT_2300 0.27 +0.1 2,330,255 - EX28DRAFT_2300 0.27 +0.2 2,330,416 - EX28DRAFT_2300 0.49 -0.0 2,330,443 + EX28DRAFT_2300 0.52 -0.4 2,330,444 - EX28DRAFT_2300 0.52 -0.3 2,330,837 + -0.5 2,330,839 + +1.3
Or see this region's nucleotide sequence