Experiment: MS_media with m-arsenite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2266 and EX28DRAFT_2267 are separated by 266 nucleotides EX28DRAFT_2267 and EX28DRAFT_2268 are separated by 15 nucleotides EX28DRAFT_2268 and EX28DRAFT_2269 overlap by 1 nucleotides EX28DRAFT_2269 and EX28DRAFT_2270 overlap by 7 nucleotides
EX28DRAFT_2266: EX28DRAFT_2266 - 2-oxoglutarate dehydrogenase, E1 component, at 2,288,679 to 2,291,486
_2266
EX28DRAFT_2267: EX28DRAFT_2267 - succinate dehydrogenase and fumarate reductase iron-sulfur protein, at 2,291,753 to 2,292,469
_2267
EX28DRAFT_2268: EX28DRAFT_2268 - succinate dehydrogenase subunit A (EC 1.3.5.1), at 2,292,485 to 2,294,251
_2268
EX28DRAFT_2269: EX28DRAFT_2269 - succinate dehydrogenase, hydrophobic membrane anchor protein, at 2,294,251 to 2,294,598
_2269
EX28DRAFT_2270: EX28DRAFT_2270 - succinate dehydrogenase subunit C (EC 1.3.5.1), at 2,294,592 to 2,295,071
_2270
Position (kb)
2292
2293
2294
2295 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 2291.507 kb on + strand at 2291.508 kb on - strand at 2291.549 kb on + strand at 2291.549 kb on + strand at 2291.550 kb on - strand at 2291.599 kb on + strand at 2291.600 kb on - strand at 2291.621 kb on - strand at 2291.755 kb on - strand at 2291.782 kb on + strand at 2291.861 kb on + strand, within EX28DRAFT_2267 at 2291.862 kb on - strand, within EX28DRAFT_2267 at 2292.022 kb on + strand, within EX28DRAFT_2267 at 2292.022 kb on + strand, within EX28DRAFT_2267 at 2292.031 kb on - strand, within EX28DRAFT_2267 at 2292.328 kb on + strand, within EX28DRAFT_2267 at 2292.353 kb on + strand, within EX28DRAFT_2267 at 2292.486 kb on + strand at 2292.493 kb on + strand at 2292.701 kb on - strand, within EX28DRAFT_2268 at 2292.854 kb on - strand, within EX28DRAFT_2268 at 2292.854 kb on - strand, within EX28DRAFT_2268 at 2292.854 kb on - strand, within EX28DRAFT_2268 at 2292.854 kb on - strand, within EX28DRAFT_2268 at 2292.854 kb on - strand, within EX28DRAFT_2268 at 2293.070 kb on + strand, within EX28DRAFT_2268 at 2293.189 kb on - strand, within EX28DRAFT_2268 at 2293.425 kb on + strand, within EX28DRAFT_2268 at 2293.627 kb on - strand, within EX28DRAFT_2268 at 2293.631 kb on - strand, within EX28DRAFT_2268 at 2293.990 kb on + strand, within EX28DRAFT_2268 at 2293.990 kb on + strand, within EX28DRAFT_2268 at 2293.990 kb on + strand, within EX28DRAFT_2268 at 2293.991 kb on - strand, within EX28DRAFT_2268 at 2294.043 kb on + strand, within EX28DRAFT_2268 at 2294.044 kb on - strand, within EX28DRAFT_2268 at 2294.063 kb on - strand, within EX28DRAFT_2268 at 2294.216 kb on + strand at 2294.279 kb on + strand at 2294.280 kb on - strand at 2294.352 kb on - strand, within EX28DRAFT_2269 at 2294.352 kb on - strand, within EX28DRAFT_2269 at 2294.502 kb on + strand, within EX28DRAFT_2269 at 2294.504 kb on + strand, within EX28DRAFT_2269 at 2294.504 kb on + strand, within EX28DRAFT_2269 at 2294.504 kb on + strand, within EX28DRAFT_2269 at 2294.505 kb on - strand, within EX28DRAFT_2269 at 2294.513 kb on + strand, within EX28DRAFT_2269 at 2294.513 kb on + strand, within EX28DRAFT_2269 at 2294.513 kb on + strand, within EX28DRAFT_2269 at 2294.560 kb on + strand, within EX28DRAFT_2269 at 2294.601 kb on - strand at 2294.690 kb on - strand, within EX28DRAFT_2270 at 2295.107 kb on + strand at 2295.193 kb on - strand at 2295.196 kb on + strand at 2295.196 kb on + strand at 2295.197 kb on - strand at 2295.197 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM remove 2,291,507 + -3.8 2,291,508 - -0.9 2,291,549 + -2.8 2,291,549 + -3.7 2,291,550 - -4.5 2,291,599 + -3.0 2,291,600 - -0.5 2,291,621 - -0.0 2,291,755 - -0.3 2,291,782 + +0.6 2,291,861 + EX28DRAFT_2267 0.15 +0.5 2,291,862 - EX28DRAFT_2267 0.15 +3.0 2,292,022 + EX28DRAFT_2267 0.38 -0.2 2,292,022 + EX28DRAFT_2267 0.38 +0.4 2,292,031 - EX28DRAFT_2267 0.39 +2.6 2,292,328 + EX28DRAFT_2267 0.80 +1.6 2,292,353 + EX28DRAFT_2267 0.84 +2.3 2,292,486 + +0.3 2,292,493 + +2.7 2,292,701 - EX28DRAFT_2268 0.12 +2.8 2,292,854 - EX28DRAFT_2268 0.21 +2.4 2,292,854 - EX28DRAFT_2268 0.21 +4.7 2,292,854 - EX28DRAFT_2268 0.21 +2.6 2,292,854 - EX28DRAFT_2268 0.21 +2.3 2,292,854 - EX28DRAFT_2268 0.21 +3.3 2,293,070 + EX28DRAFT_2268 0.33 +3.0 2,293,189 - EX28DRAFT_2268 0.40 +3.4 2,293,425 + EX28DRAFT_2268 0.53 +2.8 2,293,627 - EX28DRAFT_2268 0.65 +3.4 2,293,631 - EX28DRAFT_2268 0.65 +2.6 2,293,990 + EX28DRAFT_2268 0.85 +1.9 2,293,990 + EX28DRAFT_2268 0.85 -0.0 2,293,990 + EX28DRAFT_2268 0.85 +3.7 2,293,991 - EX28DRAFT_2268 0.85 +3.0 2,294,043 + EX28DRAFT_2268 0.88 +2.3 2,294,044 - EX28DRAFT_2268 0.88 +3.0 2,294,063 - EX28DRAFT_2268 0.89 +3.2 2,294,216 + +2.5 2,294,279 + +2.8 2,294,280 - +4.6 2,294,352 - EX28DRAFT_2269 0.29 +2.8 2,294,352 - EX28DRAFT_2269 0.29 +2.6 2,294,502 + EX28DRAFT_2269 0.72 +3.1 2,294,504 + EX28DRAFT_2269 0.73 +3.1 2,294,504 + EX28DRAFT_2269 0.73 +2.2 2,294,504 + EX28DRAFT_2269 0.73 +1.4 2,294,505 - EX28DRAFT_2269 0.73 +3.5 2,294,513 + EX28DRAFT_2269 0.75 +2.8 2,294,513 + EX28DRAFT_2269 0.75 +2.6 2,294,513 + EX28DRAFT_2269 0.75 +3.3 2,294,560 + EX28DRAFT_2269 0.89 +1.3 2,294,601 - +3.3 2,294,690 - EX28DRAFT_2270 0.20 +2.5 2,295,107 + +2.6 2,295,193 - +1.3 2,295,196 + +0.5 2,295,196 + +0.4 2,295,197 - -1.5 2,295,197 - -0.1
Or see this region's nucleotide sequence