Experiment: MS_media with m-arsenite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2018 and EX28DRAFT_2019 overlap by 4 nucleotides EX28DRAFT_2019 and EX28DRAFT_2020 are separated by 45 nucleotides EX28DRAFT_2020 and EX28DRAFT_2021 are separated by 44 nucleotides
EX28DRAFT_2018: EX28DRAFT_2018 - 3-deoxy-D-manno-octulosonate cytidylyltransferase, at 2,028,512 to 2,029,258
_2018
EX28DRAFT_2019: EX28DRAFT_2019 - Uncharacterized conserved protein, at 2,029,255 to 2,029,437
_2019
EX28DRAFT_2020: EX28DRAFT_2020 - Uncharacterized protein conserved in bacteria, at 2,029,483 to 2,030,712
_2020
EX28DRAFT_2021: EX28DRAFT_2021 - lipid-A-disaccharide kinase (EC 2.7.1.130), at 2,030,757 to 2,031,734
_2021
Position (kb)
2029
2030
2031 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2028.514 kb on - strand at 2029.469 kb on - strand at 2029.646 kb on + strand, within EX28DRAFT_2020 at 2029.772 kb on + strand, within EX28DRAFT_2020 at 2029.772 kb on + strand, within EX28DRAFT_2020 at 2029.773 kb on - strand, within EX28DRAFT_2020 at 2029.861 kb on - strand, within EX28DRAFT_2020 at 2030.316 kb on + strand, within EX28DRAFT_2020 at 2030.403 kb on + strand, within EX28DRAFT_2020 at 2030.470 kb on + strand, within EX28DRAFT_2020 at 2030.529 kb on + strand, within EX28DRAFT_2020 at 2030.543 kb on + strand, within EX28DRAFT_2020 at 2030.565 kb on + strand, within EX28DRAFT_2020 at 2030.671 kb on + strand at 2030.671 kb on + strand at 2030.672 kb on - strand at 2030.672 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM remove 2,028,514 - +0.2 2,029,469 - -0.1 2,029,646 + EX28DRAFT_2020 0.13 +0.1 2,029,772 + EX28DRAFT_2020 0.23 -0.4 2,029,772 + EX28DRAFT_2020 0.23 -1.5 2,029,773 - EX28DRAFT_2020 0.24 -0.7 2,029,861 - EX28DRAFT_2020 0.31 -0.3 2,030,316 + EX28DRAFT_2020 0.68 +0.4 2,030,403 + EX28DRAFT_2020 0.75 +2.3 2,030,470 + EX28DRAFT_2020 0.80 -2.3 2,030,529 + EX28DRAFT_2020 0.85 -0.2 2,030,543 + EX28DRAFT_2020 0.86 -2.0 2,030,565 + EX28DRAFT_2020 0.88 +1.9 2,030,671 + -0.5 2,030,671 + -1.2 2,030,672 - +0.1 2,030,672 - -1.0
Or see this region's nucleotide sequence