Experiment: MS_media with m-arsenite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0219 and EX28DRAFT_0220 are separated by 80 nucleotides EX28DRAFT_0220 and EX28DRAFT_0221 overlap by 4 nucleotides EX28DRAFT_0221 and EX28DRAFT_0222 are separated by 186 nucleotides EX28DRAFT_0222 and EX28DRAFT_0223 are separated by 3 nucleotides EX28DRAFT_0223 and EX28DRAFT_0224 are separated by 48 nucleotides
EX28DRAFT_0219: EX28DRAFT_0219 - Predicted Na+-dependent transporter, at 173,562 to 174,557
_0219
EX28DRAFT_0220: EX28DRAFT_0220 - Transcriptional regulator, at 174,638 to 175,582
_0220
EX28DRAFT_0221: EX28DRAFT_0221 - FlxA-like protein, at 175,579 to 175,917
_0221
EX28DRAFT_0222: EX28DRAFT_0222 - tRNA, at 176,104 to 176,179
_0222
EX28DRAFT_0223: EX28DRAFT_0223 - tRNA, at 176,183 to 176,258
_0223
EX28DRAFT_0224: EX28DRAFT_0224 - tRNA, at 176,307 to 176,379
_0224
Position (kb) 174
175
176 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 173.865 kb on - strand, within EX28DRAFT_0219 at 173.891 kb on + strand, within EX28DRAFT_0219 at 174.080 kb on + strand, within EX28DRAFT_0219 at 174.203 kb on + strand, within EX28DRAFT_0219 at 174.204 kb on - strand, within EX28DRAFT_0219 at 174.204 kb on - strand, within EX28DRAFT_0219 at 174.204 kb on - strand, within EX28DRAFT_0219 at 174.247 kb on + strand, within EX28DRAFT_0219 at 174.383 kb on + strand, within EX28DRAFT_0219 at 174.416 kb on + strand, within EX28DRAFT_0219 at 174.501 kb on + strand at 174.550 kb on - strand at 174.550 kb on - strand at 174.558 kb on - strand at 174.584 kb on - strand at 174.660 kb on + strand at 174.660 kb on + strand at 174.673 kb on + strand at 174.673 kb on + strand at 174.980 kb on - strand, within EX28DRAFT_0220 at 175.290 kb on + strand, within EX28DRAFT_0220 at 175.344 kb on + strand, within EX28DRAFT_0220 at 175.498 kb on + strand at 175.580 kb on + strand at 175.580 kb on + strand at 175.581 kb on - strand at 175.581 kb on - strand at 175.586 kb on + strand at 175.618 kb on - strand, within EX28DRAFT_0221 at 175.621 kb on + strand, within EX28DRAFT_0221 at 175.918 kb on - strand at 175.936 kb on - strand at 175.965 kb on - strand at 175.984 kb on + strand at 176.008 kb on - strand at 176.019 kb on - strand at 176.055 kb on - strand at 176.271 kb on + strand at 176.272 kb on - strand at 176.272 kb on - strand at 176.275 kb on + strand at 176.276 kb on - strand at 176.276 kb on - strand at 176.360 kb on - strand, within EX28DRAFT_0224 at 176.444 kb on - strand at 176.444 kb on - strand at 176.457 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM remove 173,865 - EX28DRAFT_0219 0.30 -0.4 173,891 + EX28DRAFT_0219 0.33 -0.2 174,080 + EX28DRAFT_0219 0.52 +0.1 174,203 + EX28DRAFT_0219 0.64 -1.0 174,204 - EX28DRAFT_0219 0.64 -3.3 174,204 - EX28DRAFT_0219 0.64 -0.3 174,204 - EX28DRAFT_0219 0.64 +0.3 174,247 + EX28DRAFT_0219 0.69 -0.7 174,383 + EX28DRAFT_0219 0.82 -0.2 174,416 + EX28DRAFT_0219 0.86 -1.0 174,501 + -0.4 174,550 - -0.7 174,550 - -0.8 174,558 - -0.5 174,584 - -1.0 174,660 + +0.2 174,660 + +0.6 174,673 + +0.7 174,673 + +0.1 174,980 - EX28DRAFT_0220 0.36 +0.6 175,290 + EX28DRAFT_0220 0.69 +0.2 175,344 + EX28DRAFT_0220 0.75 +0.0 175,498 + +0.8 175,580 + -1.3 175,580 + -0.2 175,581 - +0.1 175,581 - -0.4 175,586 + +0.5 175,618 - EX28DRAFT_0221 0.12 +1.1 175,621 + EX28DRAFT_0221 0.12 -0.3 175,918 - -0.5 175,936 - -0.0 175,965 - +0.1 175,984 + -0.2 176,008 - +1.1 176,019 - -0.1 176,055 - -0.8 176,271 + +0.5 176,272 - -0.4 176,272 - +0.2 176,275 + -0.3 176,276 - -0.0 176,276 - +1.0 176,360 - EX28DRAFT_0224 0.73 +0.3 176,444 - +0.1 176,444 - +0.3 176,457 - -0.6
Or see this region's nucleotide sequence