Experiment: MS_media with m-arsenite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0166 and EX28DRAFT_0167 are separated by 207 nucleotides EX28DRAFT_0167 and EX28DRAFT_0168 overlap by 4 nucleotides EX28DRAFT_0168 and EX28DRAFT_0169 are separated by 266 nucleotides
EX28DRAFT_0166: EX28DRAFT_0166 - polyphosphate kinase 1, at 113,789 to 115,849
_0166
EX28DRAFT_0167: EX28DRAFT_0167 - phosphoribosylglycinamide formyltransferase, formyltetrahydrofolate-dependent, at 116,057 to 116,698
_0167
EX28DRAFT_0168: EX28DRAFT_0168 - phosphoribosylformylglycinamidine cyclo-ligase (EC 6.3.3.1), at 116,695 to 117,732
_0168
EX28DRAFT_0169: EX28DRAFT_0169 - Beta-glucosidase/6-phospho-beta-glucosidase/beta-galactosidase, at 117,999 to 119,429
_0169
Position (kb)
116
117
118 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 115.701 kb on + strand at 115.702 kb on - strand at 115.726 kb on - strand at 115.787 kb on + strand at 115.788 kb on - strand at 115.798 kb on + strand at 115.814 kb on + strand at 115.814 kb on + strand at 115.828 kb on + strand at 115.830 kb on + strand at 115.831 kb on - strand at 115.885 kb on + strand at 115.886 kb on - strand at 115.886 kb on - strand at 115.903 kb on - strand at 115.925 kb on + strand at 115.925 kb on + strand at 115.926 kb on - strand at 115.926 kb on - strand at 116.015 kb on + strand at 116.015 kb on + strand at 116.015 kb on + strand at 116.015 kb on + strand at 116.015 kb on + strand at 116.016 kb on - strand at 116.051 kb on - strand at 116.051 kb on - strand at 116.058 kb on + strand at 116.433 kb on + strand, within EX28DRAFT_0167 at 117.757 kb on + strand at 117.758 kb on - strand at 117.758 kb on - strand at 117.762 kb on - strand at 117.762 kb on - strand at 117.762 kb on - strand at 117.762 kb on - strand at 117.919 kb on - strand at 117.919 kb on - strand at 117.919 kb on - strand at 118.010 kb on + strand at 118.260 kb on - strand, within EX28DRAFT_0169 at 118.357 kb on - strand, within EX28DRAFT_0169 at 118.357 kb on - strand, within EX28DRAFT_0169 at 118.439 kb on + strand, within EX28DRAFT_0169 at 118.439 kb on + strand, within EX28DRAFT_0169 at 118.497 kb on - strand, within EX28DRAFT_0169 at 118.507 kb on - strand, within EX28DRAFT_0169 at 118.529 kb on - strand, within EX28DRAFT_0169 at 118.532 kb on + strand, within EX28DRAFT_0169 at 118.532 kb on + strand, within EX28DRAFT_0169 at 118.532 kb on + strand, within EX28DRAFT_0169 at 118.533 kb on - strand, within EX28DRAFT_0169 at 118.533 kb on - strand, within EX28DRAFT_0169 at 118.587 kb on + strand, within EX28DRAFT_0169 at 118.724 kb on + strand, within EX28DRAFT_0169 at 118.724 kb on + strand, within EX28DRAFT_0169 at 118.725 kb on - strand, within EX28DRAFT_0169
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM remove 115,701 + -5.7 115,702 - -3.0 115,726 - -3.8 115,787 + -2.3 115,788 - -4.0 115,798 + -4.2 115,814 + -1.0 115,814 + -3.7 115,828 + -1.4 115,830 + -2.7 115,831 - -1.7 115,885 + -0.8 115,886 - -1.6 115,886 - +0.2 115,903 - +0.5 115,925 + -2.4 115,925 + +0.1 115,926 - -0.8 115,926 - +0.1 116,015 + +0.7 116,015 + -0.3 116,015 + +0.6 116,015 + -1.5 116,015 + +0.8 116,016 - +0.6 116,051 - +0.1 116,051 - +0.3 116,058 + -3.0 116,433 + EX28DRAFT_0167 0.59 -3.7 117,757 + +1.0 117,758 - -1.9 117,758 - -1.5 117,762 - -1.6 117,762 - -0.8 117,762 - -1.8 117,762 - -0.8 117,919 - +0.9 117,919 - +0.6 117,919 - +0.3 118,010 + +0.2 118,260 - EX28DRAFT_0169 0.18 +0.0 118,357 - EX28DRAFT_0169 0.25 -0.1 118,357 - EX28DRAFT_0169 0.25 -0.0 118,439 + EX28DRAFT_0169 0.31 -0.3 118,439 + EX28DRAFT_0169 0.31 +0.6 118,497 - EX28DRAFT_0169 0.35 -0.4 118,507 - EX28DRAFT_0169 0.35 -0.1 118,529 - EX28DRAFT_0169 0.37 +0.7 118,532 + EX28DRAFT_0169 0.37 +0.0 118,532 + EX28DRAFT_0169 0.37 -0.4 118,532 + EX28DRAFT_0169 0.37 +0.2 118,533 - EX28DRAFT_0169 0.37 +0.0 118,533 - EX28DRAFT_0169 0.37 +0.0 118,587 + EX28DRAFT_0169 0.41 +0.0 118,724 + EX28DRAFT_0169 0.51 -0.3 118,724 + EX28DRAFT_0169 0.51 -0.9 118,725 - EX28DRAFT_0169 0.51 -0.1
Or see this region's nucleotide sequence