Experiment: MS_media with m-arsenite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0130 and EX28DRAFT_0131 are separated by 13 nucleotides EX28DRAFT_0131 and EX28DRAFT_0132 are separated by 10 nucleotides EX28DRAFT_0132 and EX28DRAFT_0133 are separated by 112 nucleotides
EX28DRAFT_0130: EX28DRAFT_0130 - histidyl-tRNA synthetase (EC 6.1.1.21), at 73,309 to 74,583
_0130
EX28DRAFT_0131: EX28DRAFT_0131 - Uncharacterized protein conserved in bacteria, at 74,597 to 75,217
_0131
EX28DRAFT_0132: EX28DRAFT_0132 - outer membrane assembly lipoprotein YfgL, at 75,228 to 76,406
_0132
EX28DRAFT_0133: EX28DRAFT_0133 - ribosome-associated GTPase EngA, at 76,519 to 77,991
_0133
Position (kb) 75
76
77 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 74.573 kb on + strand at 74.773 kb on + strand, within EX28DRAFT_0131 at 74.853 kb on - strand, within EX28DRAFT_0131 at 74.888 kb on + strand, within EX28DRAFT_0131 at 74.896 kb on + strand, within EX28DRAFT_0131 at 74.995 kb on + strand, within EX28DRAFT_0131 at 74.995 kb on + strand, within EX28DRAFT_0131 at 75.239 kb on + strand at 75.503 kb on - strand, within EX28DRAFT_0132 at 76.117 kb on - strand, within EX28DRAFT_0132 at 76.117 kb on - strand, within EX28DRAFT_0132 at 76.260 kb on + strand, within EX28DRAFT_0132 at 76.263 kb on - strand, within EX28DRAFT_0132 at 76.405 kb on - strand at 76.409 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM remove 74,573 + -0.5 74,773 + EX28DRAFT_0131 0.28 -0.6 74,853 - EX28DRAFT_0131 0.41 -3.6 74,888 + EX28DRAFT_0131 0.47 +0.4 74,896 + EX28DRAFT_0131 0.48 -0.2 74,995 + EX28DRAFT_0131 0.64 -0.1 74,995 + EX28DRAFT_0131 0.64 +0.0 75,239 + -2.3 75,503 - EX28DRAFT_0132 0.23 -2.4 76,117 - EX28DRAFT_0132 0.75 -1.0 76,117 - EX28DRAFT_0132 0.75 -2.5 76,260 + EX28DRAFT_0132 0.88 -1.1 76,263 - EX28DRAFT_0132 0.88 -1.0 76,405 - -0.5 76,409 - +0.8
Or see this region's nucleotide sequence