Experiment: MS_media with m-arsenite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0088 and EX28DRAFT_0089 are separated by 24 nucleotides EX28DRAFT_0089 and EX28DRAFT_0090 overlap by 113 nucleotides EX28DRAFT_0090 and EX28DRAFT_0091 are separated by 135 nucleotides EX28DRAFT_0091 and EX28DRAFT_0092 are separated by 30 nucleotides
EX28DRAFT_0088: EX28DRAFT_0088 - haloacid dehalogenase superfamily, subfamily IF hydrolase, YfhB, at 27,487 to 28,122
_0088
EX28DRAFT_0089: EX28DRAFT_0089 - Cytosine/adenosine deaminases, at 28,147 to 28,686
_0089
EX28DRAFT_0090: EX28DRAFT_0090 - Predicted soluble lytic transglycosylase fused to an ABC-type amino acid-binding protein, at 28,574 to 30,124
_0090
EX28DRAFT_0091: EX28DRAFT_0091 - hypothetical protein, at 30,260 to 30,361
_0091
EX28DRAFT_0092: EX28DRAFT_0092 - phosphoribosylformylglycinamidine synthase, single chain form, at 30,392 to 34,279
_0092
Position (kb)
28
29
30
31 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 27.621 kb on - strand, within EX28DRAFT_0088 at 27.696 kb on + strand, within EX28DRAFT_0088 at 27.696 kb on + strand, within EX28DRAFT_0088 at 27.696 kb on + strand, within EX28DRAFT_0088 at 27.696 kb on + strand, within EX28DRAFT_0088 at 27.697 kb on - strand, within EX28DRAFT_0088 at 27.805 kb on + strand, within EX28DRAFT_0088 at 28.120 kb on + strand at 28.131 kb on - strand at 28.131 kb on - strand at 28.143 kb on + strand at 28.144 kb on - strand at 28.144 kb on - strand at 28.146 kb on - strand at 28.785 kb on + strand, within EX28DRAFT_0090 at 28.806 kb on + strand, within EX28DRAFT_0090 at 28.807 kb on - strand, within EX28DRAFT_0090 at 28.813 kb on - strand, within EX28DRAFT_0090 at 28.839 kb on + strand, within EX28DRAFT_0090 at 28.853 kb on - strand, within EX28DRAFT_0090 at 28.853 kb on - strand, within EX28DRAFT_0090 at 28.854 kb on + strand, within EX28DRAFT_0090 at 28.855 kb on - strand, within EX28DRAFT_0090 at 28.855 kb on - strand, within EX28DRAFT_0090 at 28.915 kb on + strand, within EX28DRAFT_0090 at 28.955 kb on - strand, within EX28DRAFT_0090 at 28.974 kb on + strand, within EX28DRAFT_0090 at 29.038 kb on + strand, within EX28DRAFT_0090 at 29.039 kb on - strand, within EX28DRAFT_0090 at 29.050 kb on - strand, within EX28DRAFT_0090 at 29.331 kb on + strand, within EX28DRAFT_0090 at 29.356 kb on + strand, within EX28DRAFT_0090 at 29.529 kb on + strand, within EX28DRAFT_0090 at 29.530 kb on - strand, within EX28DRAFT_0090 at 29.683 kb on + strand, within EX28DRAFT_0090 at 29.749 kb on - strand, within EX28DRAFT_0090 at 29.751 kb on + strand, within EX28DRAFT_0090 at 29.752 kb on - strand, within EX28DRAFT_0090 at 29.769 kb on + strand, within EX28DRAFT_0090 at 29.791 kb on + strand, within EX28DRAFT_0090 at 29.793 kb on + strand, within EX28DRAFT_0090 at 29.793 kb on + strand, within EX28DRAFT_0090 at 29.794 kb on - strand, within EX28DRAFT_0090 at 29.794 kb on - strand, within EX28DRAFT_0090 at 29.858 kb on - strand, within EX28DRAFT_0090 at 30.084 kb on - strand at 30.174 kb on + strand at 30.239 kb on + strand at 30.258 kb on + strand at 30.259 kb on - strand at 30.325 kb on + strand, within EX28DRAFT_0091 at 30.500 kb on + strand at 31.011 kb on - strand, within EX28DRAFT_0092
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM remove 27,621 - EX28DRAFT_0088 0.21 -1.2 27,696 + EX28DRAFT_0088 0.33 -0.2 27,696 + EX28DRAFT_0088 0.33 +0.2 27,696 + EX28DRAFT_0088 0.33 -0.3 27,696 + EX28DRAFT_0088 0.33 -0.1 27,697 - EX28DRAFT_0088 0.33 +0.5 27,805 + EX28DRAFT_0088 0.50 +0.6 28,120 + +0.6 28,131 - +0.1 28,131 - +0.3 28,143 + -1.0 28,144 - -0.2 28,144 - -1.0 28,146 - +0.3 28,785 + EX28DRAFT_0090 0.14 -0.1 28,806 + EX28DRAFT_0090 0.15 +0.5 28,807 - EX28DRAFT_0090 0.15 -0.6 28,813 - EX28DRAFT_0090 0.15 +0.7 28,839 + EX28DRAFT_0090 0.17 -0.6 28,853 - EX28DRAFT_0090 0.18 -0.4 28,853 - EX28DRAFT_0090 0.18 -0.4 28,854 + EX28DRAFT_0090 0.18 +0.2 28,855 - EX28DRAFT_0090 0.18 +0.4 28,855 - EX28DRAFT_0090 0.18 +0.9 28,915 + EX28DRAFT_0090 0.22 +0.0 28,955 - EX28DRAFT_0090 0.25 +0.1 28,974 + EX28DRAFT_0090 0.26 +0.0 29,038 + EX28DRAFT_0090 0.30 +1.0 29,039 - EX28DRAFT_0090 0.30 +0.7 29,050 - EX28DRAFT_0090 0.31 -0.1 29,331 + EX28DRAFT_0090 0.49 -0.4 29,356 + EX28DRAFT_0090 0.50 +0.2 29,529 + EX28DRAFT_0090 0.62 -0.4 29,530 - EX28DRAFT_0090 0.62 +0.1 29,683 + EX28DRAFT_0090 0.72 -0.2 29,749 - EX28DRAFT_0090 0.76 +0.3 29,751 + EX28DRAFT_0090 0.76 -0.0 29,752 - EX28DRAFT_0090 0.76 -0.9 29,769 + EX28DRAFT_0090 0.77 +0.1 29,791 + EX28DRAFT_0090 0.78 -0.2 29,793 + EX28DRAFT_0090 0.79 -1.1 29,793 + EX28DRAFT_0090 0.79 -0.7 29,794 - EX28DRAFT_0090 0.79 -0.5 29,794 - EX28DRAFT_0090 0.79 +1.0 29,858 - EX28DRAFT_0090 0.83 +0.8 30,084 - +0.3 30,174 + +0.5 30,239 + +0.0 30,258 + -1.3 30,259 - -3.4 30,325 + EX28DRAFT_0091 0.64 -3.5 30,500 + +0.2 31,011 - EX28DRAFT_0092 0.16 +1.0
Or see this region's nucleotide sequence