Experiment: MS_media with m-arsenite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0079 and EX28DRAFT_0080 overlap by 4 nucleotides EX28DRAFT_0080 and EX28DRAFT_0081 are separated by 17 nucleotides EX28DRAFT_0081 and EX28DRAFT_0082 are separated by 75 nucleotides EX28DRAFT_0082 and EX28DRAFT_0083 overlap by 1 nucleotides
EX28DRAFT_0079: EX28DRAFT_0079 - ribonuclease III, bacterial, at 20,453 to 21,133
_0079
EX28DRAFT_0080: EX28DRAFT_0080 - GTP-binding protein Era, at 21,130 to 22,035
_0080
EX28DRAFT_0081: EX28DRAFT_0081 - DNA replication and repair protein RecO, at 22,053 to 22,760
_0081
EX28DRAFT_0082: EX28DRAFT_0082 - pyridoxine 5'-phosphate synthase, at 22,836 to 23,567
_0082
EX28DRAFT_0083: EX28DRAFT_0083 - holo-[acyl-carrier-protein] synthase, at 23,567 to 23,947
_0083
Position (kb)
22
23 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 22.038 kb on + strand at 22.038 kb on + strand at 22.052 kb on + strand at 22.086 kb on - strand at 22.086 kb on - strand at 22.086 kb on - strand at 22.113 kb on - strand at 22.225 kb on + strand, within EX28DRAFT_0081 at 22.323 kb on + strand, within EX28DRAFT_0081 at 22.489 kb on + strand, within EX28DRAFT_0081 at 22.526 kb on - strand, within EX28DRAFT_0081 at 22.626 kb on + strand, within EX28DRAFT_0081 at 22.626 kb on + strand, within EX28DRAFT_0081 at 22.886 kb on + strand at 23.376 kb on + strand, within EX28DRAFT_0082
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM remove 22,038 + -0.2 22,038 + +1.6 22,052 + +0.3 22,086 - -0.9 22,086 - -2.6 22,086 - -0.4 22,113 - +1.0 22,225 + EX28DRAFT_0081 0.24 -3.1 22,323 + EX28DRAFT_0081 0.38 -0.4 22,489 + EX28DRAFT_0081 0.62 -0.6 22,526 - EX28DRAFT_0081 0.67 -1.6 22,626 + EX28DRAFT_0081 0.81 +0.2 22,626 + EX28DRAFT_0081 0.81 +2.0 22,886 + -0.8 23,376 + EX28DRAFT_0082 0.74 -0.6
Or see this region's nucleotide sequence