Experiment: MS_media with m-arsenite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0072 and EX28DRAFT_0073 are separated by 31 nucleotides EX28DRAFT_0073 and EX28DRAFT_0074 overlap by 1 nucleotides EX28DRAFT_0074 and EX28DRAFT_0075 overlap by 4 nucleotides
EX28DRAFT_0072: EX28DRAFT_0072 - RNA polymerase sigma factor RpoE, at 14,572 to 15,147
_0072
EX28DRAFT_0073: EX28DRAFT_0073 - Negative regulator of sigma E activity, at 15,179 to 15,829
_0073
EX28DRAFT_0074: EX28DRAFT_0074 - sigma E regulatory protein, MucB/RseB, at 15,829 to 16,782
_0074
EX28DRAFT_0075: EX28DRAFT_0075 - positive regulator of sigma(E), RseC/MucC, at 16,779 to 17,255
_0075
Position (kb) 15
16 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 14.206 kb on + strand at 14.206 kb on + strand at 14.206 kb on + strand at 14.206 kb on + strand at 14.207 kb on - strand at 14.207 kb on - strand at 14.207 kb on - strand at 14.207 kb on - strand at 14.272 kb on - strand at 14.328 kb on + strand at 14.329 kb on - strand at 14.406 kb on + strand at 14.406 kb on + strand at 14.406 kb on + strand at 14.406 kb on + strand at 14.407 kb on - strand at 15.120 kb on + strand at 15.542 kb on - strand, within EX28DRAFT_0073 at 15.544 kb on + strand, within EX28DRAFT_0073 at 15.970 kb on - strand, within EX28DRAFT_0074 at 15.981 kb on + strand, within EX28DRAFT_0074 at 15.982 kb on - strand, within EX28DRAFT_0074 at 16.049 kb on + strand, within EX28DRAFT_0074 at 16.050 kb on - strand, within EX28DRAFT_0074 at 16.145 kb on - strand, within EX28DRAFT_0074 at 16.146 kb on + strand, within EX28DRAFT_0074 at 16.147 kb on - strand, within EX28DRAFT_0074 at 16.174 kb on + strand, within EX28DRAFT_0074 at 16.204 kb on + strand, within EX28DRAFT_0074 at 16.212 kb on + strand, within EX28DRAFT_0074 at 16.288 kb on + strand, within EX28DRAFT_0074 at 16.294 kb on + strand, within EX28DRAFT_0074 at 16.294 kb on + strand, within EX28DRAFT_0074 at 16.294 kb on + strand, within EX28DRAFT_0074 at 16.294 kb on + strand, within EX28DRAFT_0074 at 16.294 kb on + strand, within EX28DRAFT_0074 at 16.294 kb on + strand, within EX28DRAFT_0074 at 16.295 kb on - strand, within EX28DRAFT_0074 at 16.295 kb on - strand, within EX28DRAFT_0074 at 16.295 kb on - strand, within EX28DRAFT_0074 at 16.295 kb on - strand, within EX28DRAFT_0074 at 16.295 kb on - strand, within EX28DRAFT_0074 at 16.295 kb on - strand, within EX28DRAFT_0074 at 16.482 kb on + strand, within EX28DRAFT_0074 at 16.585 kb on + strand, within EX28DRAFT_0074 at 16.618 kb on - strand, within EX28DRAFT_0074 at 16.680 kb on + strand, within EX28DRAFT_0074 at 16.681 kb on - strand, within EX28DRAFT_0074 at 16.761 kb on + strand at 16.761 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM remove 14,206 + +0.0 14,206 + -0.3 14,206 + +0.4 14,206 + +0.5 14,207 - -0.2 14,207 - +0.5 14,207 - -1.0 14,207 - +0.4 14,272 - +0.3 14,328 + +0.8 14,329 - +1.0 14,406 + -0.7 14,406 + +0.9 14,406 + -0.2 14,406 + +0.5 14,407 - +0.6 15,120 + +2.3 15,542 - EX28DRAFT_0073 0.56 -1.8 15,544 + EX28DRAFT_0073 0.56 +2.0 15,970 - EX28DRAFT_0074 0.15 -1.2 15,981 + EX28DRAFT_0074 0.16 -2.8 15,982 - EX28DRAFT_0074 0.16 -1.9 16,049 + EX28DRAFT_0074 0.23 +0.4 16,050 - EX28DRAFT_0074 0.23 -0.6 16,145 - EX28DRAFT_0074 0.33 -2.7 16,146 + EX28DRAFT_0074 0.33 -2.7 16,147 - EX28DRAFT_0074 0.33 -4.0 16,174 + EX28DRAFT_0074 0.36 -2.6 16,204 + EX28DRAFT_0074 0.39 -2.9 16,212 + EX28DRAFT_0074 0.40 -1.1 16,288 + EX28DRAFT_0074 0.48 -1.9 16,294 + EX28DRAFT_0074 0.49 -3.3 16,294 + EX28DRAFT_0074 0.49 -0.4 16,294 + EX28DRAFT_0074 0.49 -1.3 16,294 + EX28DRAFT_0074 0.49 -0.5 16,294 + EX28DRAFT_0074 0.49 +0.6 16,294 + EX28DRAFT_0074 0.49 +1.0 16,295 - EX28DRAFT_0074 0.49 +2.0 16,295 - EX28DRAFT_0074 0.49 -1.0 16,295 - EX28DRAFT_0074 0.49 -2.5 16,295 - EX28DRAFT_0074 0.49 -1.4 16,295 - EX28DRAFT_0074 0.49 -1.5 16,295 - EX28DRAFT_0074 0.49 -1.6 16,482 + EX28DRAFT_0074 0.68 -4.5 16,585 + EX28DRAFT_0074 0.79 -0.6 16,618 - EX28DRAFT_0074 0.83 -1.6 16,680 + EX28DRAFT_0074 0.89 -0.8 16,681 - EX28DRAFT_0074 0.89 -2.0 16,761 + -0.8 16,761 + +0.4
Or see this region's nucleotide sequence