Strain Fitness in Escherichia coli BW25113 around b2465

Experiment: Glycolic Acid (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nttalA and tktB are separated by 19 nucleotidestktB and ypfG are separated by 94 nucleotides b2464: talA - transaldolase A (NCBI), at 2,576,688 to 2,577,638 talA b2465: tktB - transketolase 2, thiamin-binding (NCBI), at 2,577,658 to 2,579,661 tktB b2466: ypfG - hypothetical protein (NCBI), at 2,579,756 to 2,580,799 ypfG Position (kb) 2577 2578 2579 2580Strain fitness (log2 ratio) -1 0 1 2 3 4at 2576.853 kb on - strand, within talAat 2576.984 kb on - strand, within talAat 2577.042 kb on - strand, within talAat 2577.044 kb on + strand, within talAat 2577.044 kb on + strand, within talAat 2577.060 kb on - strand, within talAat 2577.060 kb on - strand, within talAat 2577.263 kb on + strand, within talAat 2577.266 kb on + strand, within talAat 2577.491 kb on - strand, within talAat 2577.659 kb on - strandat 2577.696 kb on + strandat 2577.704 kb on - strandat 2577.706 kb on - strandat 2577.706 kb on - strandat 2577.856 kb on - strandat 2577.856 kb on - strandat 2578.022 kb on + strand, within tktBat 2578.083 kb on - strand, within tktBat 2578.083 kb on - strand, within tktBat 2578.169 kb on + strand, within tktBat 2578.352 kb on + strand, within tktBat 2578.372 kb on + strand, within tktBat 2578.439 kb on + strand, within tktBat 2578.439 kb on + strand, within tktBat 2578.624 kb on + strand, within tktBat 2578.725 kb on + strand, within tktBat 2578.725 kb on + strand, within tktBat 2578.865 kb on + strand, within tktBat 2579.050 kb on - strand, within tktBat 2579.050 kb on - strand, within tktBat 2579.050 kb on - strand, within tktBat 2579.050 kb on - strand, within tktBat 2579.079 kb on - strand, within tktBat 2579.181 kb on + strand, within tktBat 2579.205 kb on - strand, within tktBat 2579.369 kb on + strand, within tktBat 2579.406 kb on - strand, within tktBat 2579.406 kb on - strand, within tktBat 2579.438 kb on - strand, within tktBat 2579.438 kb on - strand, within tktBat 2579.586 kb on + strandat 2579.702 kb on - strandat 2579.799 kb on - strandat 2579.799 kb on - strandat 2579.825 kb on + strandat 2579.825 kb on + strandat 2580.163 kb on - strand, within ypfGat 2580.219 kb on + strand, within ypfGat 2580.222 kb on - strand, within ypfGat 2580.222 kb on - strand, within ypfGat 2580.223 kb on - strand, within ypfGat 2580.258 kb on - strand, within ypfGat 2580.264 kb on - strand, within ypfGat 2580.517 kb on + strand, within ypfGat 2580.572 kb on + strand, within ypfGat 2580.609 kb on + strand, within ypfGat 2580.609 kb on + strand, within ypfG

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Per-strain Table

Position Strand Gene LocusTag Fraction Glycolic Acid (C)
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2,576,853 - talA b2464 0.17 +3.9
2,576,984 - talA b2464 0.31 +2.5
2,577,042 - talA b2464 0.37 +3.1
2,577,044 + talA b2464 0.37 +0.6
2,577,044 + talA b2464 0.37 +0.7
2,577,060 - talA b2464 0.39 +3.1
2,577,060 - talA b2464 0.39 +2.9
2,577,263 + talA b2464 0.60 -0.3
2,577,266 + talA b2464 0.61 +0.8
2,577,491 - talA b2464 0.84 +0.1
2,577,659 - +1.0
2,577,696 + +0.3
2,577,704 - +1.1
2,577,706 - +0.1
2,577,706 - -0.9
2,577,856 - -0.4
2,577,856 - -1.3
2,578,022 + tktB b2465 0.18 +0.7
2,578,083 - tktB b2465 0.21 +0.4
2,578,083 - tktB b2465 0.21 +1.0
2,578,169 + tktB b2465 0.25 +0.3
2,578,352 + tktB b2465 0.35 +0.6
2,578,372 + tktB b2465 0.36 +0.1
2,578,439 + tktB b2465 0.39 -0.4
2,578,439 + tktB b2465 0.39 +1.0
2,578,624 + tktB b2465 0.48 +0.5
2,578,725 + tktB b2465 0.53 +0.1
2,578,725 + tktB b2465 0.53 -0.6
2,578,865 + tktB b2465 0.60 +1.4
2,579,050 - tktB b2465 0.69 +0.4
2,579,050 - tktB b2465 0.69 -0.2
2,579,050 - tktB b2465 0.69 +0.8
2,579,050 - tktB b2465 0.69 -1.2
2,579,079 - tktB b2465 0.71 -0.8
2,579,181 + tktB b2465 0.76 -1.0
2,579,205 - tktB b2465 0.77 +0.6
2,579,369 + tktB b2465 0.85 +0.2
2,579,406 - tktB b2465 0.87 -0.1
2,579,406 - tktB b2465 0.87 +1.1
2,579,438 - tktB b2465 0.89 +0.5
2,579,438 - tktB b2465 0.89 +0.6
2,579,586 + +0.1
2,579,702 - -1.1
2,579,799 - +0.3
2,579,799 - +1.3
2,579,825 + +0.7
2,579,825 + +0.3
2,580,163 - ypfG b2466 0.39 -0.1
2,580,219 + ypfG b2466 0.44 -1.2
2,580,222 - ypfG b2466 0.45 -1.0
2,580,222 - ypfG b2466 0.45 +1.4
2,580,223 - ypfG b2466 0.45 +0.9
2,580,258 - ypfG b2466 0.48 +0.4
2,580,264 - ypfG b2466 0.49 +0.7
2,580,517 + ypfG b2466 0.73 +0.8
2,580,572 + ypfG b2466 0.78 +0.7
2,580,609 + ypfG b2466 0.82 -0.0
2,580,609 + ypfG b2466 0.82 +1.1

Or see this region's nucleotide sequence