Strain Fitness in Escherichia coli BW25113 around b2400

Experiment: Glycolic Acid (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyfeC and yfeD are separated by 1 nucleotidesyfeD and gltX are separated by 51 nucleotidesgltX and valU are separated by 258 nucleotidesvalU and valX are separated by 44 nucleotidesvalX and valY are separated by 46 nucleotidesvalY and lysV are separated by 4 nucleotideslysV and xapR are separated by 264 nucleotides b2398: yfeC - orf, hypothetical protein (VIMSS), at 2,516,489 to 2,516,833 yfeC b2399: yfeD - predicted DNA-binding transcriptional regulator (RefSeq), at 2,516,835 to 2,517,227 yfeD b2400: gltX - glutamyl-tRNA synthetase (NCBI), at 2,517,279 to 2,518,694 gltX b2401: valU - tRNA-Val (NCBI), at 2,518,953 to 2,519,028 valU b2402: valX - tRNA-Val (NCBI), at 2,519,073 to 2,519,148 valX b2403: valY - tRNA-Val (NCBI), at 2,519,195 to 2,519,270 valY b2404: lysV - tRNA-Lys (NCBI), at 2,519,275 to 2,519,350 lysV b2405: xapR - DNA-binding transcriptional activator (NCBI), at 2,519,615 to 2,520,499 xapR Position (kb) 2517 2518 2519Strain fitness (log2 ratio) -1 0 1 2 3at 2516.434 kb on - strandat 2516.439 kb on + strandat 2516.439 kb on + strandat 2516.560 kb on - strand, within yfeCat 2516.560 kb on - strand, within yfeCat 2516.568 kb on - strand, within yfeCat 2516.620 kb on - strand, within yfeCat 2516.787 kb on + strand, within yfeCat 2516.808 kb on + strandat 2516.808 kb on + strandat 2516.873 kb on + strandat 2516.873 kb on + strandat 2516.890 kb on - strand, within yfeDat 2516.890 kb on - strand, within yfeDat 2516.963 kb on - strand, within yfeDat 2516.976 kb on - strand, within yfeDat 2517.017 kb on - strand, within yfeDat 2517.027 kb on + strand, within yfeDat 2517.035 kb on - strand, within yfeDat 2517.035 kb on - strand, within yfeDat 2517.035 kb on - strand, within yfeDat 2517.037 kb on - strand, within yfeDat 2517.044 kb on + strand, within yfeDat 2517.044 kb on + strand, within yfeDat 2517.047 kb on + strand, within yfeDat 2517.047 kb on + strand, within yfeDat 2517.203 kb on + strandat 2517.213 kb on + strandat 2517.213 kb on + strandat 2517.281 kb on - strandat 2518.799 kb on + strandat 2518.935 kb on - strandat 2518.935 kb on - strandat 2518.939 kb on + strandat 2519.620 kb on - strandat 2519.657 kb on + strandat 2519.665 kb on - strandat 2519.679 kb on + strandat 2519.687 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Glycolic Acid (C)
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2,516,434 - -0.6
2,516,439 + -0.5
2,516,439 + -0.4
2,516,560 - yfeC b2398 0.21 -1.3
2,516,560 - yfeC b2398 0.21 -0.3
2,516,568 - yfeC b2398 0.23 -1.1
2,516,620 - yfeC b2398 0.38 -0.1
2,516,787 + yfeC b2398 0.86 -0.9
2,516,808 + -0.7
2,516,808 + -0.2
2,516,873 + +0.6
2,516,873 + +0.5
2,516,890 - yfeD b2399 0.14 +0.1
2,516,890 - yfeD b2399 0.14 -0.6
2,516,963 - yfeD b2399 0.33 -1.3
2,516,976 - yfeD b2399 0.36 -0.3
2,517,017 - yfeD b2399 0.46 +0.1
2,517,027 + yfeD b2399 0.49 -1.0
2,517,035 - yfeD b2399 0.51 +0.6
2,517,035 - yfeD b2399 0.51 -0.8
2,517,035 - yfeD b2399 0.51 -0.4
2,517,037 - yfeD b2399 0.51 -0.1
2,517,044 + yfeD b2399 0.53 -1.7
2,517,044 + yfeD b2399 0.53 -1.0
2,517,047 + yfeD b2399 0.54 -0.1
2,517,047 + yfeD b2399 0.54 -1.0
2,517,203 + +0.9
2,517,213 + -0.7
2,517,213 + +0.2
2,517,281 - +0.6
2,518,799 + -0.4
2,518,935 - +2.0
2,518,935 - +3.4
2,518,939 + +0.7
2,519,620 - -1.5
2,519,657 + +0.6
2,519,665 - +1.1
2,519,679 + +0.2
2,519,687 - +1.7

Or see this region's nucleotide sequence