Experiment: Glycolic Acid (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yfeA and alaX are separated by 208 nucleotides alaX and alaW are separated by 39 nucleotides alaW and yfeC are separated by 235 nucleotides yfeC and yfeD are separated by 1 nucleotides yfeD and gltX are separated by 51 nucleotides
b2395: yfeA - orf, hypothetical protein (VIMSS), at 2,513,665 to 2,515,854
yfeA
b2396: alaX - tRNA-Ala (NCBI), at 2,516,063 to 2,516,138
alaX
b2397: alaW - tRNA-Ala (NCBI), at 2,516,178 to 2,516,253
alaW
b2398: yfeC - orf, hypothetical protein (VIMSS), at 2,516,489 to 2,516,833
yfeC
b2399: yfeD - predicted DNA-binding transcriptional regulator (RefSeq), at 2,516,835 to 2,517,227
yfeD
b2400: gltX - glutamyl-tRNA synthetase (NCBI), at 2,517,279 to 2,518,694
gltX
Position (kb)
2516
2517
2518 Strain fitness (log2 ratio)
-1
0
1 at 2515.857 kb on + strand at 2515.859 kb on + strand at 2515.859 kb on + strand at 2515.867 kb on - strand at 2515.977 kb on - strand at 2515.986 kb on - strand at 2516.197 kb on - strand, within alaW at 2516.434 kb on - strand at 2516.439 kb on + strand at 2516.439 kb on + strand at 2516.560 kb on - strand, within yfeC at 2516.560 kb on - strand, within yfeC at 2516.568 kb on - strand, within yfeC at 2516.620 kb on - strand, within yfeC at 2516.787 kb on + strand, within yfeC at 2516.808 kb on + strand at 2516.808 kb on + strand at 2516.873 kb on + strand at 2516.873 kb on + strand at 2516.890 kb on - strand, within yfeD at 2516.890 kb on - strand, within yfeD at 2516.963 kb on - strand, within yfeD at 2516.976 kb on - strand, within yfeD at 2517.017 kb on - strand, within yfeD at 2517.027 kb on + strand, within yfeD at 2517.035 kb on - strand, within yfeD at 2517.035 kb on - strand, within yfeD at 2517.035 kb on - strand, within yfeD at 2517.037 kb on - strand, within yfeD at 2517.044 kb on + strand, within yfeD at 2517.044 kb on + strand, within yfeD at 2517.047 kb on + strand, within yfeD at 2517.047 kb on + strand, within yfeD at 2517.203 kb on + strand at 2517.213 kb on + strand at 2517.213 kb on + strand at 2517.281 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Glycolic Acid (C) remove 2,515,857 + -0.2 2,515,859 + +1.1 2,515,859 + -0.4 2,515,867 - +0.3 2,515,977 - +0.5 2,515,986 - +0.2 2,516,197 - alaW b2397 0.25 -0.9 2,516,434 - -0.6 2,516,439 + -0.5 2,516,439 + -0.4 2,516,560 - yfeC b2398 0.21 -1.3 2,516,560 - yfeC b2398 0.21 -0.3 2,516,568 - yfeC b2398 0.23 -1.1 2,516,620 - yfeC b2398 0.38 -0.1 2,516,787 + yfeC b2398 0.86 -0.9 2,516,808 + -0.7 2,516,808 + -0.2 2,516,873 + +0.6 2,516,873 + +0.5 2,516,890 - yfeD b2399 0.14 +0.1 2,516,890 - yfeD b2399 0.14 -0.6 2,516,963 - yfeD b2399 0.33 -1.3 2,516,976 - yfeD b2399 0.36 -0.3 2,517,017 - yfeD b2399 0.46 +0.1 2,517,027 + yfeD b2399 0.49 -1.0 2,517,035 - yfeD b2399 0.51 +0.6 2,517,035 - yfeD b2399 0.51 -0.8 2,517,035 - yfeD b2399 0.51 -0.4 2,517,037 - yfeD b2399 0.51 -0.1 2,517,044 + yfeD b2399 0.53 -1.7 2,517,044 + yfeD b2399 0.53 -1.0 2,517,047 + yfeD b2399 0.54 -0.1 2,517,047 + yfeD b2399 0.54 -1.0 2,517,203 + +0.9 2,517,213 + -0.7 2,517,213 + +0.2 2,517,281 - +0.6
Or see this region's nucleotide sequence