Strain Fitness in Escherichia coli BW25113 around b2140

Experiment: Glycolic Acid (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntmdtQ and yohP are separated by 223 nucleotidesyohP and dusC are separated by 372 nucleotidesdusC and yohJ are separated by 238 nucleotidesyohJ and yohK overlap by 4 nucleotides b2139: mdtQ - orf, hypothetical protein (VIMSS), at 2,225,345 to 2,226,780 mdtQ b4679: yohP - expressed protein (RefSeq), at 2,227,004 to 2,227,087 yohP b2140: dusC - tRNA-dihydrouridine synthase C (NCBI), at 2,227,460 to 2,228,407 dusC b2141: yohJ - hypothetical protein (NCBI), at 2,228,646 to 2,229,044 yohJ b2142: yohK - predicted inner membrane protein (NCBI), at 2,229,041 to 2,229,736 yohK Position (kb) 2227 2228 2229Strain fitness (log2 ratio) -2 -1 0 1 2 3at 2226.528 kb on + strand, within mdtQat 2226.528 kb on + strand, within mdtQat 2226.668 kb on - strandat 2226.721 kb on + strandat 2226.738 kb on + strandat 2226.753 kb on + strandat 2226.753 kb on + strandat 2226.782 kb on + strandat 2226.782 kb on + strandat 2226.794 kb on + strandat 2226.903 kb on + strandat 2226.934 kb on + strandat 2226.934 kb on + strandat 2226.942 kb on - strandat 2227.010 kb on + strandat 2227.020 kb on + strand, within yohPat 2227.020 kb on + strand, within yohPat 2227.047 kb on + strand, within yohPat 2227.047 kb on + strand, within yohPat 2227.050 kb on + strand, within yohPat 2227.051 kb on + strand, within yohPat 2227.052 kb on + strand, within yohPat 2227.052 kb on + strand, within yohPat 2227.052 kb on + strand, within yohPat 2227.126 kb on + strandat 2227.144 kb on + strandat 2227.158 kb on - strandat 2227.158 kb on - strandat 2227.163 kb on - strandat 2227.167 kb on + strandat 2227.168 kb on - strandat 2227.175 kb on - strandat 2227.179 kb on - strandat 2227.179 kb on - strandat 2227.336 kb on + strandat 2227.336 kb on + strandat 2227.345 kb on + strandat 2227.355 kb on + strandat 2227.395 kb on + strandat 2227.432 kb on + strandat 2227.592 kb on - strand, within dusCat 2227.617 kb on - strand, within dusCat 2227.620 kb on - strand, within dusCat 2227.620 kb on - strand, within dusCat 2227.663 kb on + strand, within dusCat 2227.663 kb on + strand, within dusCat 2227.671 kb on - strand, within dusCat 2227.671 kb on - strand, within dusCat 2227.671 kb on - strand, within dusCat 2227.671 kb on - strand, within dusCat 2227.671 kb on - strand, within dusCat 2227.674 kb on + strand, within dusCat 2227.675 kb on - strand, within dusCat 2227.676 kb on - strand, within dusCat 2227.747 kb on - strand, within dusCat 2227.752 kb on - strand, within dusCat 2227.752 kb on - strand, within dusCat 2227.767 kb on + strand, within dusCat 2227.767 kb on + strand, within dusCat 2227.791 kb on - strand, within dusCat 2227.791 kb on - strand, within dusCat 2227.791 kb on - strand, within dusCat 2227.808 kb on - strand, within dusCat 2227.808 kb on - strand, within dusCat 2227.862 kb on + strand, within dusCat 2227.862 kb on + strand, within dusCat 2227.891 kb on - strand, within dusCat 2227.918 kb on - strand, within dusCat 2228.138 kb on + strand, within dusCat 2228.327 kb on - strandat 2228.444 kb on - strandat 2228.444 kb on - strandat 2228.446 kb on - strandat 2228.446 kb on - strandat 2228.530 kb on - strandat 2228.542 kb on - strandat 2228.542 kb on - strandat 2228.637 kb on - strandat 2228.637 kb on - strandat 2228.716 kb on - strand, within yohJat 2228.716 kb on - strand, within yohJat 2228.753 kb on + strand, within yohJat 2228.753 kb on + strand, within yohJat 2228.951 kb on + strand, within yohJat 2229.015 kb on + strandat 2229.068 kb on - strandat 2229.123 kb on - strand, within yohKat 2229.123 kb on - strand, within yohKat 2229.123 kb on - strand, within yohKat 2229.123 kb on - strand, within yohKat 2229.190 kb on + strand, within yohKat 2229.221 kb on + strand, within yohKat 2229.221 kb on + strand, within yohKat 2229.259 kb on + strand, within yohKat 2229.272 kb on - strand, within yohKat 2229.279 kb on + strand, within yohKat 2229.279 kb on + strand, within yohKat 2229.284 kb on + strand, within yohKat 2229.284 kb on + strand, within yohKat 2229.292 kb on - strand, within yohK

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Per-strain Table

Position Strand Gene LocusTag Fraction Glycolic Acid (C)
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2,226,528 + mdtQ b2139 0.82 +0.1
2,226,528 + mdtQ b2139 0.82 +0.0
2,226,668 - +0.2
2,226,721 + -0.8
2,226,738 + -1.5
2,226,753 + +0.3
2,226,753 + +0.4
2,226,782 + -0.2
2,226,782 + +0.3
2,226,794 + -0.6
2,226,903 + +0.6
2,226,934 + -0.9
2,226,934 + +2.0
2,226,942 - -1.7
2,227,010 + +0.4
2,227,020 + yohP b4679 0.19 +1.3
2,227,020 + yohP b4679 0.19 +1.7
2,227,047 + yohP b4679 0.51 +0.7
2,227,047 + yohP b4679 0.51 -0.6
2,227,050 + yohP b4679 0.55 +0.3
2,227,051 + yohP b4679 0.56 +2.8
2,227,052 + yohP b4679 0.57 +0.2
2,227,052 + yohP b4679 0.57 -0.3
2,227,052 + yohP b4679 0.57 +1.0
2,227,126 + +1.1
2,227,144 + +0.7
2,227,158 - +0.2
2,227,158 - +0.1
2,227,163 - +0.4
2,227,167 + -1.5
2,227,168 - -1.3
2,227,175 - +0.5
2,227,179 - -1.9
2,227,179 - -0.5
2,227,336 + -0.2
2,227,336 + -0.8
2,227,345 + +0.2
2,227,355 + +1.1
2,227,395 + -0.2
2,227,432 + -1.2
2,227,592 - dusC b2140 0.14 -0.8
2,227,617 - dusC b2140 0.17 +0.4
2,227,620 - dusC b2140 0.17 +0.4
2,227,620 - dusC b2140 0.17 +0.8
2,227,663 + dusC b2140 0.21 -1.1
2,227,663 + dusC b2140 0.21 -0.3
2,227,671 - dusC b2140 0.22 +0.5
2,227,671 - dusC b2140 0.22 +0.9
2,227,671 - dusC b2140 0.22 +0.8
2,227,671 - dusC b2140 0.22 +0.7
2,227,671 - dusC b2140 0.22 +1.2
2,227,674 + dusC b2140 0.23 -0.2
2,227,675 - dusC b2140 0.23 +0.6
2,227,676 - dusC b2140 0.23 +0.4
2,227,747 - dusC b2140 0.30 +0.2
2,227,752 - dusC b2140 0.31 +0.1
2,227,752 - dusC b2140 0.31 +2.3
2,227,767 + dusC b2140 0.32 +1.9
2,227,767 + dusC b2140 0.32 +0.2
2,227,791 - dusC b2140 0.35 +1.0
2,227,791 - dusC b2140 0.35 -0.3
2,227,791 - dusC b2140 0.35 +0.7
2,227,808 - dusC b2140 0.37 +0.7
2,227,808 - dusC b2140 0.37 -0.5
2,227,862 + dusC b2140 0.42 +0.6
2,227,862 + dusC b2140 0.42 +0.4
2,227,891 - dusC b2140 0.45 +0.7
2,227,918 - dusC b2140 0.48 +0.7
2,228,138 + dusC b2140 0.72 -1.5
2,228,327 - +0.9
2,228,444 - -0.5
2,228,444 - -0.9
2,228,446 - +0.1
2,228,446 - -0.8
2,228,530 - -1.4
2,228,542 - +0.8
2,228,542 - +0.7
2,228,637 - +0.1
2,228,637 - +0.2
2,228,716 - yohJ b2141 0.18 +1.4
2,228,716 - yohJ b2141 0.18 +2.0
2,228,753 + yohJ b2141 0.27 +2.7
2,228,753 + yohJ b2141 0.27 +1.9
2,228,951 + yohJ b2141 0.76 +1.7
2,229,015 + -0.1
2,229,068 - +1.3
2,229,123 - yohK b2142 0.12 +1.0
2,229,123 - yohK b2142 0.12 +1.2
2,229,123 - yohK b2142 0.12 +1.0
2,229,123 - yohK b2142 0.12 +1.0
2,229,190 + yohK b2142 0.21 +0.8
2,229,221 + yohK b2142 0.26 +0.7
2,229,221 + yohK b2142 0.26 +1.7
2,229,259 + yohK b2142 0.31 +1.7
2,229,272 - yohK b2142 0.33 +0.4
2,229,279 + yohK b2142 0.34 -1.6
2,229,279 + yohK b2142 0.34 +0.5
2,229,284 + yohK b2142 0.35 +2.4
2,229,284 + yohK b2142 0.35 +1.1
2,229,292 - yohK b2142 0.36 +2.5

Or see this region's nucleotide sequence