Experiment: Glycolic Acid (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yaeF and proS are separated by 53 nucleotides proS and yaeB are separated by 111 nucleotides yaeB and rcsF overlap by 4 nucleotides
b0193: yaeF - orf, hypothetical protein (VIMSS), at 216,179 to 217,003
yaeF
b0194: proS - prolyl-tRNA synthetase (NCBI), at 217,057 to 218,775
proS
b0195: yaeB - hypothetical protein (NCBI), at 218,887 to 219,594
yaeB
b0196: rcsF - predicted outer membrane protein, signal (NCBI), at 219,591 to 219,995
rcsF
Position (kb) 217
218
219 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 216.101 kb on - strand at 216.101 kb on - strand at 216.101 kb on - strand at 216.151 kb on + strand at 216.159 kb on - strand at 216.159 kb on - strand at 216.160 kb on - strand at 216.160 kb on - strand at 216.235 kb on + strand at 216.235 kb on + strand at 216.282 kb on + strand, within yaeF at 216.285 kb on + strand, within yaeF at 216.285 kb on + strand, within yaeF at 216.326 kb on - strand, within yaeF at 216.332 kb on - strand, within yaeF at 216.332 kb on - strand, within yaeF at 216.334 kb on + strand, within yaeF at 216.334 kb on + strand, within yaeF at 216.342 kb on - strand, within yaeF at 216.342 kb on - strand, within yaeF at 216.417 kb on + strand, within yaeF at 216.417 kb on - strand, within yaeF at 216.417 kb on - strand, within yaeF at 216.444 kb on + strand, within yaeF at 216.505 kb on + strand, within yaeF at 216.635 kb on + strand, within yaeF at 216.733 kb on + strand, within yaeF at 216.749 kb on - strand, within yaeF at 216.774 kb on - strand, within yaeF at 216.786 kb on - strand, within yaeF at 216.804 kb on + strand, within yaeF at 216.868 kb on - strand, within yaeF at 216.896 kb on + strand, within yaeF at 216.896 kb on + strand, within yaeF at 217.010 kb on - strand at 217.354 kb on + strand, within proS at 218.821 kb on - strand at 218.871 kb on + strand at 218.910 kb on + strand at 218.910 kb on + strand at 218.965 kb on + strand, within yaeB at 218.974 kb on - strand, within yaeB at 219.027 kb on - strand, within yaeB at 219.071 kb on - strand, within yaeB at 219.200 kb on + strand, within yaeB at 219.240 kb on + strand, within yaeB at 219.240 kb on + strand, within yaeB at 219.261 kb on + strand, within yaeB at 219.312 kb on + strand, within yaeB at 219.446 kb on - strand, within yaeB at 219.465 kb on + strand, within yaeB at 219.466 kb on + strand, within yaeB at 219.489 kb on - strand, within yaeB at 219.513 kb on + strand, within yaeB at 219.548 kb on - strand at 219.598 kb on + strand at 219.598 kb on + strand at 219.643 kb on - strand, within rcsF at 219.678 kb on + strand, within rcsF at 219.680 kb on + strand, within rcsF at 219.680 kb on + strand, within rcsF at 219.680 kb on + strand, within rcsF at 219.720 kb on - strand, within rcsF at 219.720 kb on - strand, within rcsF at 219.756 kb on + strand, within rcsF at 219.764 kb on - strand, within rcsF at 219.764 kb on - strand, within rcsF at 219.764 kb on - strand, within rcsF
Per-strain Table
Position Strand Gene LocusTag Fraction Glycolic Acid (C) remove 216,101 - -0.1 216,101 - -0.5 216,101 - -1.1 216,151 + -0.3 216,159 - +0.5 216,159 - -0.5 216,160 - +0.1 216,160 - +0.4 216,235 + -2.2 216,235 + -0.2 216,282 + yaeF b0193 0.12 +1.0 216,285 + yaeF b0193 0.13 +0.4 216,285 + yaeF b0193 0.13 -0.1 216,326 - yaeF b0193 0.18 -0.4 216,332 - yaeF b0193 0.19 -0.2 216,332 - yaeF b0193 0.19 -1.2 216,334 + yaeF b0193 0.19 +0.3 216,334 + yaeF b0193 0.19 +0.4 216,342 - yaeF b0193 0.20 +0.7 216,342 - yaeF b0193 0.20 +0.3 216,417 + yaeF b0193 0.29 -0.2 216,417 - yaeF b0193 0.29 +0.7 216,417 - yaeF b0193 0.29 -0.9 216,444 + yaeF b0193 0.32 -1.1 216,505 + yaeF b0193 0.40 +0.0 216,635 + yaeF b0193 0.55 -0.4 216,733 + yaeF b0193 0.67 -0.7 216,749 - yaeF b0193 0.69 -0.1 216,774 - yaeF b0193 0.72 -0.5 216,786 - yaeF b0193 0.74 +0.8 216,804 + yaeF b0193 0.76 +0.4 216,868 - yaeF b0193 0.84 +0.2 216,896 + yaeF b0193 0.87 +0.8 216,896 + yaeF b0193 0.87 +1.0 217,010 - +0.1 217,354 + proS b0194 0.17 +4.4 218,821 - -0.2 218,871 + +1.1 218,910 + +1.0 218,910 + +1.3 218,965 + yaeB b0195 0.11 +0.2 218,974 - yaeB b0195 0.12 -0.0 219,027 - yaeB b0195 0.20 -0.6 219,071 - yaeB b0195 0.26 +0.4 219,200 + yaeB b0195 0.44 +0.8 219,240 + yaeB b0195 0.50 -1.7 219,240 + yaeB b0195 0.50 +0.6 219,261 + yaeB b0195 0.53 -0.5 219,312 + yaeB b0195 0.60 +0.5 219,446 - yaeB b0195 0.79 +0.2 219,465 + yaeB b0195 0.82 +1.2 219,466 + yaeB b0195 0.82 -1.6 219,489 - yaeB b0195 0.85 -0.9 219,513 + yaeB b0195 0.88 +1.0 219,548 - -0.8 219,598 + -0.8 219,598 + -1.5 219,643 - rcsF b0196 0.13 +0.9 219,678 + rcsF b0196 0.21 -0.4 219,680 + rcsF b0196 0.22 +1.2 219,680 + rcsF b0196 0.22 +0.8 219,680 + rcsF b0196 0.22 +1.5 219,720 - rcsF b0196 0.32 -0.7 219,720 - rcsF b0196 0.32 -0.0 219,756 + rcsF b0196 0.41 +1.3 219,764 - rcsF b0196 0.43 +0.6 219,764 - rcsF b0196 0.43 +0.7 219,764 - rcsF b0196 0.43 +1.3
Or see this region's nucleotide sequence