Experiment: MS_media with m-arsenite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_4530 and EX28DRAFT_4531 are separated by 336 nucleotides EX28DRAFT_4531 and EX28DRAFT_4532 overlap by 4 nucleotides EX28DRAFT_4532 and EX28DRAFT_4533 are separated by 1 nucleotides EX28DRAFT_4533 and EX28DRAFT_4534 overlap by 17 nucleotides
EX28DRAFT_4530: EX28DRAFT_4530 - FOG: GGDEF domain, at 13,383 to 14,924
_4530
EX28DRAFT_4531: EX28DRAFT_4531 - thiazole biosynthesis protein ThiH, at 15,261 to 16,388
_4531
EX28DRAFT_4532: EX28DRAFT_4532 - Uncharacterized enzyme of thiazole biosynthesis, at 16,385 to 17,155
_4532
EX28DRAFT_4533: EX28DRAFT_4533 - thiamine biosynthesis protein ThiS, at 17,157 to 17,357
_4533
EX28DRAFT_4534: EX28DRAFT_4534 - Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2, at 17,341 to 18,096
_4534
Position (kb)
15
16
17 Strain fitness (log2 ratio)
-1
0
1
2 at 14.407 kb on + strand, within EX28DRAFT_4530 at 14.456 kb on + strand, within EX28DRAFT_4530 at 14.457 kb on - strand, within EX28DRAFT_4530 at 14.526 kb on - strand, within EX28DRAFT_4530 at 14.544 kb on + strand, within EX28DRAFT_4530 at 14.544 kb on + strand, within EX28DRAFT_4530 at 14.564 kb on - strand, within EX28DRAFT_4530 at 14.695 kb on + strand, within EX28DRAFT_4530 at 14.717 kb on + strand, within EX28DRAFT_4530 at 14.718 kb on - strand, within EX28DRAFT_4530 at 14.719 kb on + strand, within EX28DRAFT_4530 at 14.720 kb on - strand, within EX28DRAFT_4530 at 14.720 kb on - strand, within EX28DRAFT_4530 at 14.720 kb on - strand, within EX28DRAFT_4530 at 14.931 kb on + strand at 14.931 kb on + strand at 14.931 kb on + strand at 14.931 kb on + strand at 14.931 kb on + strand at 14.932 kb on - strand at 14.953 kb on - strand at 14.982 kb on + strand at 14.983 kb on - strand at 15.012 kb on - strand at 15.013 kb on + strand at 15.014 kb on - strand at 15.042 kb on + strand at 15.043 kb on - strand at 15.048 kb on - strand at 15.069 kb on + strand at 15.070 kb on - strand at 15.678 kb on - strand, within EX28DRAFT_4531 at 15.678 kb on - strand, within EX28DRAFT_4531 at 15.679 kb on + strand, within EX28DRAFT_4531 at 15.679 kb on + strand, within EX28DRAFT_4531 at 15.680 kb on - strand, within EX28DRAFT_4531 at 15.680 kb on - strand, within EX28DRAFT_4531 at 15.889 kb on + strand, within EX28DRAFT_4531 at 15.889 kb on + strand, within EX28DRAFT_4531 at 15.890 kb on - strand, within EX28DRAFT_4531 at 15.890 kb on - strand, within EX28DRAFT_4531 at 15.970 kb on + strand, within EX28DRAFT_4531 at 16.083 kb on - strand, within EX28DRAFT_4531 at 16.117 kb on + strand, within EX28DRAFT_4531 at 16.117 kb on + strand, within EX28DRAFT_4531 at 16.150 kb on + strand, within EX28DRAFT_4531 at 16.150 kb on + strand, within EX28DRAFT_4531 at 16.163 kb on - strand, within EX28DRAFT_4531 at 16.163 kb on - strand, within EX28DRAFT_4531 at 16.163 kb on - strand, within EX28DRAFT_4531 at 17.015 kb on + strand, within EX28DRAFT_4532 at 17.016 kb on - strand, within EX28DRAFT_4532 at 17.147 kb on - strand at 17.147 kb on - strand at 17.151 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM remove 14,407 + EX28DRAFT_4530 0.66 -0.1 14,456 + EX28DRAFT_4530 0.70 +0.1 14,457 - EX28DRAFT_4530 0.70 +0.7 14,526 - EX28DRAFT_4530 0.74 +1.4 14,544 + EX28DRAFT_4530 0.75 +0.4 14,544 + EX28DRAFT_4530 0.75 +0.3 14,564 - EX28DRAFT_4530 0.77 +0.5 14,695 + EX28DRAFT_4530 0.85 -0.2 14,717 + EX28DRAFT_4530 0.87 +0.4 14,718 - EX28DRAFT_4530 0.87 -0.3 14,719 + EX28DRAFT_4530 0.87 -0.1 14,720 - EX28DRAFT_4530 0.87 +0.1 14,720 - EX28DRAFT_4530 0.87 +0.5 14,720 - EX28DRAFT_4530 0.87 -0.6 14,931 + +0.3 14,931 + -0.1 14,931 + -0.5 14,931 + -0.2 14,931 + -0.2 14,932 - -0.9 14,953 - +0.1 14,982 + -0.1 14,983 - -0.7 15,012 - +0.7 15,013 + +1.2 15,014 - -0.1 15,042 + +0.1 15,043 - +1.7 15,048 - +0.1 15,069 + +0.3 15,070 - -0.2 15,678 - EX28DRAFT_4531 0.37 +2.4 15,678 - EX28DRAFT_4531 0.37 +0.7 15,679 + EX28DRAFT_4531 0.37 +0.2 15,679 + EX28DRAFT_4531 0.37 +0.8 15,680 - EX28DRAFT_4531 0.37 -0.7 15,680 - EX28DRAFT_4531 0.37 -0.0 15,889 + EX28DRAFT_4531 0.56 +0.6 15,889 + EX28DRAFT_4531 0.56 -0.2 15,890 - EX28DRAFT_4531 0.56 +0.4 15,890 - EX28DRAFT_4531 0.56 -0.0 15,970 + EX28DRAFT_4531 0.63 -0.4 16,083 - EX28DRAFT_4531 0.73 +0.0 16,117 + EX28DRAFT_4531 0.76 +0.9 16,117 + EX28DRAFT_4531 0.76 -0.3 16,150 + EX28DRAFT_4531 0.79 +0.0 16,150 + EX28DRAFT_4531 0.79 -0.3 16,163 - EX28DRAFT_4531 0.80 +0.3 16,163 - EX28DRAFT_4531 0.80 +0.4 16,163 - EX28DRAFT_4531 0.80 +0.6 17,015 + EX28DRAFT_4532 0.82 -0.4 17,016 - EX28DRAFT_4532 0.82 +0.3 17,147 - +0.0 17,147 - +0.2 17,151 - +0.4
Or see this region's nucleotide sequence