Experiment: MS_media with m-arsenite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_4290 and EX28DRAFT_4291 are separated by 152 nucleotides EX28DRAFT_4291 and EX28DRAFT_4292 are separated by 119 nucleotides EX28DRAFT_4292 and EX28DRAFT_4293 are separated by 116 nucleotides EX28DRAFT_4293 and EX28DRAFT_4294 are separated by 203 nucleotides
EX28DRAFT_4290: EX28DRAFT_4290 - ABC-type branched-chain amino acid transport systems, periplasmic component, at 94,111 to 95,220
_4290
EX28DRAFT_4291: EX28DRAFT_4291 - hypothetical protein, at 95,373 to 95,525
_4291
EX28DRAFT_4292: EX28DRAFT_4292 - Acetyltransferase (GNAT) domain, at 95,645 to 96,034
_4292
EX28DRAFT_4293: EX28DRAFT_4293 - ABC-type branched-chain amino acid transport systems, periplasmic component, at 96,151 to 97,245
_4293
EX28DRAFT_4294: EX28DRAFT_4294 - 4-aminobutyrate aminotransferase, prokaryotic type, at 97,449 to 98,714
_4294
Position (kb)
96
97
98 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 95.262 kb on + strand at 95.263 kb on - strand at 95.271 kb on + strand at 95.272 kb on - strand at 95.396 kb on - strand, within EX28DRAFT_4291 at 95.597 kb on + strand at 95.597 kb on + strand at 95.598 kb on - strand at 95.606 kb on + strand at 95.617 kb on + strand at 95.639 kb on + strand at 95.639 kb on + strand at 95.639 kb on + strand at 95.639 kb on + strand at 95.639 kb on + strand at 95.643 kb on + strand at 95.720 kb on + strand, within EX28DRAFT_4292 at 95.721 kb on - strand, within EX28DRAFT_4292 at 95.762 kb on + strand, within EX28DRAFT_4292 at 95.762 kb on + strand, within EX28DRAFT_4292 at 95.777 kb on - strand, within EX28DRAFT_4292 at 95.883 kb on - strand, within EX28DRAFT_4292 at 95.887 kb on - strand, within EX28DRAFT_4292 at 95.887 kb on - strand, within EX28DRAFT_4292 at 96.345 kb on - strand, within EX28DRAFT_4293 at 96.345 kb on - strand, within EX28DRAFT_4293 at 96.405 kb on + strand, within EX28DRAFT_4293 at 96.405 kb on + strand, within EX28DRAFT_4293 at 96.405 kb on + strand, within EX28DRAFT_4293 at 96.590 kb on + strand, within EX28DRAFT_4293 at 96.738 kb on - strand, within EX28DRAFT_4293 at 96.795 kb on - strand, within EX28DRAFT_4293 at 96.907 kb on - strand, within EX28DRAFT_4293 at 96.920 kb on + strand, within EX28DRAFT_4293 at 96.920 kb on + strand, within EX28DRAFT_4293 at 96.921 kb on - strand, within EX28DRAFT_4293 at 96.971 kb on + strand, within EX28DRAFT_4293 at 96.971 kb on + strand, within EX28DRAFT_4293 at 96.971 kb on + strand, within EX28DRAFT_4293 at 96.972 kb on - strand, within EX28DRAFT_4293 at 96.972 kb on - strand, within EX28DRAFT_4293 at 97.041 kb on - strand, within EX28DRAFT_4293 at 97.080 kb on + strand, within EX28DRAFT_4293 at 97.080 kb on + strand, within EX28DRAFT_4293 at 97.087 kb on - strand, within EX28DRAFT_4293 at 97.215 kb on + strand at 97.216 kb on - strand at 97.279 kb on + strand at 97.280 kb on - strand at 97.407 kb on + strand at 97.409 kb on + strand at 97.409 kb on + strand at 97.409 kb on + strand at 97.409 kb on + strand at 97.410 kb on - strand at 97.410 kb on - strand at 97.415 kb on - strand at 97.451 kb on - strand at 97.473 kb on - strand at 97.687 kb on + strand, within EX28DRAFT_4294 at 97.689 kb on - strand, within EX28DRAFT_4294 at 97.825 kb on + strand, within EX28DRAFT_4294 at 97.825 kb on + strand, within EX28DRAFT_4294 at 98.055 kb on - strand, within EX28DRAFT_4294 at 98.145 kb on - strand, within EX28DRAFT_4294 at 98.218 kb on + strand, within EX28DRAFT_4294 at 98.218 kb on + strand, within EX28DRAFT_4294
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM remove 95,262 + +1.4 95,263 - -0.7 95,271 + +1.6 95,272 - -1.5 95,396 - EX28DRAFT_4291 0.15 -1.2 95,597 + +0.5 95,597 + +0.6 95,598 - -2.3 95,606 + +0.9 95,617 + -0.1 95,639 + -1.6 95,639 + -1.6 95,639 + -2.5 95,639 + -1.4 95,639 + -0.6 95,643 + +0.0 95,720 + EX28DRAFT_4292 0.19 -2.7 95,721 - EX28DRAFT_4292 0.19 -3.0 95,762 + EX28DRAFT_4292 0.30 -1.7 95,762 + EX28DRAFT_4292 0.30 -0.7 95,777 - EX28DRAFT_4292 0.34 -3.3 95,883 - EX28DRAFT_4292 0.61 -3.7 95,887 - EX28DRAFT_4292 0.62 -3.1 95,887 - EX28DRAFT_4292 0.62 -2.1 96,345 - EX28DRAFT_4293 0.18 +1.6 96,345 - EX28DRAFT_4293 0.18 +1.1 96,405 + EX28DRAFT_4293 0.23 +1.3 96,405 + EX28DRAFT_4293 0.23 +1.3 96,405 + EX28DRAFT_4293 0.23 +1.5 96,590 + EX28DRAFT_4293 0.40 +1.2 96,738 - EX28DRAFT_4293 0.54 +1.5 96,795 - EX28DRAFT_4293 0.59 +1.5 96,907 - EX28DRAFT_4293 0.69 +1.0 96,920 + EX28DRAFT_4293 0.70 +0.6 96,920 + EX28DRAFT_4293 0.70 +0.9 96,921 - EX28DRAFT_4293 0.70 +1.8 96,971 + EX28DRAFT_4293 0.75 +1.0 96,971 + EX28DRAFT_4293 0.75 +1.3 96,971 + EX28DRAFT_4293 0.75 +0.5 96,972 - EX28DRAFT_4293 0.75 +1.4 96,972 - EX28DRAFT_4293 0.75 +1.9 97,041 - EX28DRAFT_4293 0.81 +1.3 97,080 + EX28DRAFT_4293 0.85 +1.2 97,080 + EX28DRAFT_4293 0.85 +1.0 97,087 - EX28DRAFT_4293 0.85 +1.5 97,215 + -0.3 97,216 - +1.6 97,279 + +1.6 97,280 - +0.1 97,407 + -0.2 97,409 + +0.9 97,409 + -0.1 97,409 + -0.8 97,409 + +0.1 97,410 - +0.4 97,410 - -0.1 97,415 - -0.8 97,451 - +0.1 97,473 - -0.4 97,687 + EX28DRAFT_4294 0.19 -0.2 97,689 - EX28DRAFT_4294 0.19 +0.3 97,825 + EX28DRAFT_4294 0.30 -0.1 97,825 + EX28DRAFT_4294 0.30 -1.2 98,055 - EX28DRAFT_4294 0.48 -0.6 98,145 - EX28DRAFT_4294 0.55 -0.5 98,218 + EX28DRAFT_4294 0.61 -0.4 98,218 + EX28DRAFT_4294 0.61 -0.1
Or see this region's nucleotide sequence