Experiment: MS_media with m-arsenite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3602 and EX28DRAFT_3603 are separated by 55 nucleotides EX28DRAFT_3603 and EX28DRAFT_3604 are separated by 27 nucleotides EX28DRAFT_3604 and EX28DRAFT_3605 are separated by 50 nucleotides EX28DRAFT_3605 and EX28DRAFT_3606 overlap by 4 nucleotides
EX28DRAFT_3602: EX28DRAFT_3602 - Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains), at 261,273 to 263,210
_3602
EX28DRAFT_3603: EX28DRAFT_3603 - Trp operon repressor, at 263,266 to 263,595
_3603
EX28DRAFT_3604: EX28DRAFT_3604 - inosine/xanthosine triphosphatase, at 263,623 to 264,138
_3604
EX28DRAFT_3605: EX28DRAFT_3605 - Fructose-2,6-bisphosphatase, at 264,189 to 264,836
_3605
EX28DRAFT_3606: EX28DRAFT_3606 - AraC-type DNA-binding domain-containing proteins, at 264,833 to 265,702
_3606
Position (kb) 264
265 Strain fitness (log2 ratio) -2
-1
0
1
2 at 263.227 kb on + strand at 263.262 kb on + strand at 263.263 kb on - strand at 263.263 kb on - strand at 263.284 kb on + strand at 263.577 kb on - strand at 263.577 kb on - strand at 263.617 kb on - strand at 263.678 kb on + strand, within EX28DRAFT_3604 at 263.733 kb on + strand, within EX28DRAFT_3604 at 263.759 kb on + strand, within EX28DRAFT_3604 at 263.760 kb on - strand, within EX28DRAFT_3604 at 264.111 kb on + strand at 264.138 kb on + strand at 264.138 kb on + strand at 264.138 kb on + strand at 264.138 kb on + strand at 264.139 kb on - strand at 264.143 kb on - strand at 264.172 kb on + strand at 264.173 kb on - strand at 264.173 kb on - strand at 264.173 kb on - strand at 264.174 kb on + strand at 264.174 kb on + strand at 264.201 kb on + strand at 264.201 kb on + strand at 264.202 kb on - strand at 264.202 kb on - strand at 264.202 kb on - strand at 264.465 kb on - strand, within EX28DRAFT_3605 at 264.465 kb on - strand, within EX28DRAFT_3605 at 264.563 kb on + strand, within EX28DRAFT_3605 at 265.072 kb on + strand, within EX28DRAFT_3606 at 265.072 kb on + strand, within EX28DRAFT_3606 at 265.073 kb on - strand, within EX28DRAFT_3606 at 265.075 kb on - strand, within EX28DRAFT_3606 at 265.111 kb on - strand, within EX28DRAFT_3606 at 265.269 kb on + strand, within EX28DRAFT_3606 at 265.269 kb on + strand, within EX28DRAFT_3606 at 265.270 kb on - strand, within EX28DRAFT_3606 at 265.270 kb on - strand, within EX28DRAFT_3606 at 265.270 kb on - strand, within EX28DRAFT_3606 at 265.270 kb on - strand, within EX28DRAFT_3606 at 265.320 kb on + strand, within EX28DRAFT_3606 at 265.527 kb on - strand, within EX28DRAFT_3606 at 265.543 kb on + strand, within EX28DRAFT_3606 at 265.544 kb on - strand, within EX28DRAFT_3606 at 265.545 kb on + strand, within EX28DRAFT_3606 at 265.546 kb on - strand, within EX28DRAFT_3606 at 265.546 kb on - strand, within EX28DRAFT_3606 at 265.546 kb on - strand, within EX28DRAFT_3606 at 265.702 kb on + strand at 265.702 kb on + strand at 265.702 kb on + strand at 265.702 kb on + strand at 265.703 kb on - strand at 265.703 kb on - strand at 265.703 kb on - strand at 265.703 kb on - strand at 265.703 kb on - strand at 265.703 kb on - strand at 265.703 kb on - strand at 265.704 kb on + strand at 265.704 kb on + strand at 265.704 kb on + strand at 265.704 kb on + strand at 265.704 kb on + strand at 265.704 kb on + strand at 265.704 kb on + strand at 265.704 kb on + strand at 265.704 kb on + strand at 265.705 kb on - strand at 265.705 kb on - strand at 265.705 kb on - strand at 265.705 kb on - strand at 265.705 kb on - strand at 265.705 kb on - strand at 265.705 kb on - strand at 265.719 kb on + strand at 265.719 kb on + strand at 265.719 kb on + strand at 265.756 kb on + strand at 265.831 kb on - strand at 265.832 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM remove 263,227 + -0.0 263,262 + -0.8 263,263 - -1.0 263,263 - +0.1 263,284 + -0.7 263,577 - -0.4 263,577 - -1.0 263,617 - -0.1 263,678 + EX28DRAFT_3604 0.11 -0.4 263,733 + EX28DRAFT_3604 0.21 -0.1 263,759 + EX28DRAFT_3604 0.26 +1.7 263,760 - EX28DRAFT_3604 0.27 -2.3 264,111 + -0.2 264,138 + +2.2 264,138 + -0.4 264,138 + -0.3 264,138 + -0.2 264,139 - -1.2 264,143 - +0.2 264,172 + -0.7 264,173 - -1.1 264,173 - -0.5 264,173 - -0.6 264,174 + -0.1 264,174 + +0.1 264,201 + -1.1 264,201 + -0.8 264,202 - -0.4 264,202 - -0.5 264,202 - -0.9 264,465 - EX28DRAFT_3605 0.43 -1.1 264,465 - EX28DRAFT_3605 0.43 +0.3 264,563 + EX28DRAFT_3605 0.58 -0.9 265,072 + EX28DRAFT_3606 0.27 +0.3 265,072 + EX28DRAFT_3606 0.27 +0.8 265,073 - EX28DRAFT_3606 0.28 -0.2 265,075 - EX28DRAFT_3606 0.28 +0.2 265,111 - EX28DRAFT_3606 0.32 +1.9 265,269 + EX28DRAFT_3606 0.50 -0.3 265,269 + EX28DRAFT_3606 0.50 +0.3 265,270 - EX28DRAFT_3606 0.50 +1.0 265,270 - EX28DRAFT_3606 0.50 +0.8 265,270 - EX28DRAFT_3606 0.50 -0.1 265,270 - EX28DRAFT_3606 0.50 +1.1 265,320 + EX28DRAFT_3606 0.56 +0.5 265,527 - EX28DRAFT_3606 0.80 +0.7 265,543 + EX28DRAFT_3606 0.82 +0.2 265,544 - EX28DRAFT_3606 0.82 +0.0 265,545 + EX28DRAFT_3606 0.82 -0.2 265,546 - EX28DRAFT_3606 0.82 +0.6 265,546 - EX28DRAFT_3606 0.82 +0.6 265,546 - EX28DRAFT_3606 0.82 -0.2 265,702 + +1.8 265,702 + -1.3 265,702 + +1.1 265,702 + -0.2 265,703 - -0.2 265,703 - -2.1 265,703 - -0.0 265,703 - +0.1 265,703 - -0.5 265,703 - +1.5 265,703 - -0.2 265,704 + +0.0 265,704 + +0.3 265,704 + -0.2 265,704 + -0.0 265,704 + +0.0 265,704 + +0.2 265,704 + -0.0 265,704 + -0.4 265,704 + +0.0 265,705 - +1.1 265,705 - +0.3 265,705 - +0.0 265,705 - +0.6 265,705 - +0.2 265,705 - +0.4 265,705 - -0.2 265,719 + -0.5 265,719 + +0.2 265,719 + -1.9 265,756 + -0.5 265,831 - +1.0 265,832 + -0.1
Or see this region's nucleotide sequence