Experiment: MS_media with m-arsenite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3032 and EX28DRAFT_3033 are separated by 141 nucleotides EX28DRAFT_3033 and EX28DRAFT_3034 are separated by 10 nucleotides EX28DRAFT_3034 and EX28DRAFT_3035 are separated by 179 nucleotides EX28DRAFT_3035 and EX28DRAFT_3036 are separated by 52 nucleotides EX28DRAFT_3036 and EX28DRAFT_3037 are separated by 155 nucleotides
EX28DRAFT_3032: EX28DRAFT_3032 - Sugar phosphate isomerases/epimerases, at 343,274 to 344,329
_3032
EX28DRAFT_3033: EX28DRAFT_3033 - transcriptional regulator, TetR family, at 344,471 to 345,061
_3033
EX28DRAFT_3034: EX28DRAFT_3034 - hypothetical protein, at 345,072 to 345,350
_3034
EX28DRAFT_3035: EX28DRAFT_3035 - hypothetical protein, at 345,530 to 345,979
_3035
EX28DRAFT_3036: EX28DRAFT_3036 - RNA, at 346,032 to 346,118
_3036
EX28DRAFT_3037: EX28DRAFT_3037 - hypothetical protein, at 346,274 to 346,528
_3037
Position (kb) 345
346 Strain fitness (log2 ratio) -2
-1
0
1 at 344.131 kb on + strand, within EX28DRAFT_3032 at 344.263 kb on + strand at 344.332 kb on + strand at 344.332 kb on + strand at 344.332 kb on + strand at 344.333 kb on - strand at 344.333 kb on - strand at 344.334 kb on + strand at 344.334 kb on + strand at 344.335 kb on - strand at 344.335 kb on - strand at 344.371 kb on - strand at 344.397 kb on + strand at 344.426 kb on - strand at 344.426 kb on - strand at 344.546 kb on + strand, within EX28DRAFT_3033 at 344.547 kb on - strand, within EX28DRAFT_3033 at 344.607 kb on - strand, within EX28DRAFT_3033 at 344.785 kb on + strand, within EX28DRAFT_3033 at 344.785 kb on + strand, within EX28DRAFT_3033 at 344.785 kb on + strand, within EX28DRAFT_3033 at 344.785 kb on + strand, within EX28DRAFT_3033 at 344.785 kb on + strand, within EX28DRAFT_3033 at 344.790 kb on + strand, within EX28DRAFT_3033 at 344.951 kb on + strand, within EX28DRAFT_3033 at 344.951 kb on + strand, within EX28DRAFT_3033 at 345.175 kb on + strand, within EX28DRAFT_3034 at 345.242 kb on + strand, within EX28DRAFT_3034 at 345.243 kb on - strand, within EX28DRAFT_3034 at 345.247 kb on - strand, within EX28DRAFT_3034 at 345.247 kb on - strand, within EX28DRAFT_3034 at 345.251 kb on + strand, within EX28DRAFT_3034 at 345.251 kb on + strand, within EX28DRAFT_3034 at 345.251 kb on + strand, within EX28DRAFT_3034 at 345.252 kb on - strand, within EX28DRAFT_3034 at 345.306 kb on - strand, within EX28DRAFT_3034 at 345.331 kb on - strand at 345.331 kb on - strand at 345.338 kb on - strand at 345.338 kb on - strand at 345.384 kb on - strand at 345.409 kb on + strand at 345.431 kb on - strand at 345.451 kb on + strand at 345.452 kb on - strand at 345.453 kb on + strand at 345.477 kb on + strand at 345.532 kb on - strand at 345.532 kb on - strand at 345.595 kb on - strand, within EX28DRAFT_3035 at 345.690 kb on + strand, within EX28DRAFT_3035 at 345.691 kb on - strand, within EX28DRAFT_3035 at 345.879 kb on + strand, within EX28DRAFT_3035 at 345.886 kb on - strand, within EX28DRAFT_3035 at 346.061 kb on + strand, within EX28DRAFT_3036 at 346.062 kb on - strand, within EX28DRAFT_3036 at 346.090 kb on + strand, within EX28DRAFT_3036 at 346.139 kb on + strand at 346.139 kb on + strand at 346.140 kb on - strand at 346.140 kb on - strand at 346.140 kb on - strand at 346.140 kb on - strand at 346.140 kb on - strand at 346.216 kb on + strand at 346.279 kb on - strand at 346.279 kb on - strand at 346.282 kb on + strand at 346.282 kb on + strand at 346.282 kb on + strand at 346.282 kb on + strand at 346.283 kb on - strand at 346.283 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM remove 344,131 + EX28DRAFT_3032 0.81 -0.2 344,263 + +0.6 344,332 + +0.5 344,332 + +0.1 344,332 + -0.3 344,333 - -1.5 344,333 - +0.6 344,334 + -0.3 344,334 + +0.2 344,335 - -0.5 344,335 - +0.3 344,371 - +0.5 344,397 + -0.3 344,426 - -0.0 344,426 - +0.4 344,546 + EX28DRAFT_3033 0.13 -0.5 344,547 - EX28DRAFT_3033 0.13 -0.5 344,607 - EX28DRAFT_3033 0.23 -0.3 344,785 + EX28DRAFT_3033 0.53 -1.3 344,785 + EX28DRAFT_3033 0.53 -1.0 344,785 + EX28DRAFT_3033 0.53 -1.3 344,785 + EX28DRAFT_3033 0.53 -0.7 344,785 + EX28DRAFT_3033 0.53 +0.3 344,790 + EX28DRAFT_3033 0.54 -1.0 344,951 + EX28DRAFT_3033 0.81 -0.5 344,951 + EX28DRAFT_3033 0.81 -0.8 345,175 + EX28DRAFT_3034 0.37 -0.3 345,242 + EX28DRAFT_3034 0.61 -1.0 345,243 - EX28DRAFT_3034 0.61 -0.3 345,247 - EX28DRAFT_3034 0.63 -0.2 345,247 - EX28DRAFT_3034 0.63 -1.7 345,251 + EX28DRAFT_3034 0.64 -0.1 345,251 + EX28DRAFT_3034 0.64 -0.4 345,251 + EX28DRAFT_3034 0.64 -0.5 345,252 - EX28DRAFT_3034 0.65 +0.1 345,306 - EX28DRAFT_3034 0.84 -0.4 345,331 - -0.5 345,331 - -2.0 345,338 - -0.4 345,338 - -0.6 345,384 - +0.3 345,409 + +0.2 345,431 - -0.7 345,451 + -0.9 345,452 - +1.2 345,453 + -0.2 345,477 + -0.2 345,532 - -0.7 345,532 - -0.8 345,595 - EX28DRAFT_3035 0.14 +0.1 345,690 + EX28DRAFT_3035 0.36 -0.5 345,691 - EX28DRAFT_3035 0.36 -0.9 345,879 + EX28DRAFT_3035 0.78 +0.1 345,886 - EX28DRAFT_3035 0.79 -0.7 346,061 + EX28DRAFT_3036 0.33 +0.2 346,062 - EX28DRAFT_3036 0.34 -0.4 346,090 + EX28DRAFT_3036 0.67 -1.5 346,139 + -0.2 346,139 + -0.3 346,140 - -0.4 346,140 - -0.7 346,140 - -0.4 346,140 - -0.5 346,140 - -0.0 346,216 + +0.8 346,279 - +0.3 346,279 - +0.5 346,282 + +0.6 346,282 + +0.2 346,282 + -1.0 346,282 + -0.7 346,283 - -0.6 346,283 - -0.4
Or see this region's nucleotide sequence