Strain Fitness in Escherichia coli BW25113 around b3424

Experiment: Glycolic Acid (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrtcR and glpR overlap by 19 nucleotidesglpR and glpG are separated by 16 nucleotidesglpG and glpE are separated by 44 nucleotidesglpE and glpD are separated by 189 nucleotides b3422: rtcR - sigma 54-dependent transcriptional regulator of rtcBA expression (NCBI), at 3,556,290 to 3,557,888 rtcR b3423: glpR - DNA-binding transcriptional repressor (NCBI), at 3,557,870 to 3,558,628 glpR b3424: glpG - predicted intramembrane serine protease (NCBI), at 3,558,645 to 3,559,475 glpG b3425: glpE - thiosulfate sulfurtransferase (NCBI), at 3,559,520 to 3,559,846 glpE b3426: glpD - sn-glycerol-3-phosphate dehydrogenase, aerobic, FAD/NAD(P)-binding (NCBI), at 3,560,036 to 3,561,541 glpD Position (kb) 3558 3559 3560Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 3557.673 kb on + strand, within rtcRat 3557.709 kb on - strand, within rtcRat 3557.709 kb on - strand, within rtcRat 3557.833 kb on - strandat 3557.849 kb on + strandat 3557.849 kb on + strandat 3557.881 kb on + strandat 3557.884 kb on + strandat 3557.908 kb on - strandat 3557.908 kb on - strandat 3557.948 kb on - strand, within glpRat 3557.949 kb on + strand, within glpRat 3557.959 kb on + strand, within glpRat 3557.968 kb on + strand, within glpRat 3558.019 kb on + strand, within glpRat 3558.019 kb on + strand, within glpRat 3558.069 kb on + strand, within glpRat 3558.069 kb on + strand, within glpRat 3558.101 kb on + strand, within glpRat 3558.103 kb on - strand, within glpRat 3558.103 kb on - strand, within glpRat 3558.103 kb on - strand, within glpRat 3558.104 kb on - strand, within glpRat 3558.104 kb on - strand, within glpRat 3558.115 kb on + strand, within glpRat 3558.143 kb on - strand, within glpRat 3558.156 kb on - strand, within glpRat 3558.156 kb on - strand, within glpRat 3558.221 kb on - strand, within glpRat 3558.266 kb on + strand, within glpRat 3558.301 kb on + strand, within glpRat 3558.309 kb on - strand, within glpRat 3558.309 kb on - strand, within glpRat 3558.309 kb on - strand, within glpRat 3558.309 kb on - strand, within glpRat 3558.309 kb on - strand, within glpRat 3558.327 kb on + strand, within glpRat 3558.364 kb on - strand, within glpRat 3558.364 kb on - strand, within glpRat 3558.407 kb on + strand, within glpRat 3558.409 kb on + strand, within glpRat 3558.415 kb on + strand, within glpRat 3558.523 kb on - strand, within glpRat 3558.572 kb on - strandat 3558.578 kb on + strandat 3558.578 kb on + strandat 3558.588 kb on - strandat 3558.606 kb on + strandat 3558.687 kb on + strandat 3558.753 kb on + strand, within glpGat 3558.766 kb on + strand, within glpGat 3558.774 kb on - strand, within glpGat 3558.776 kb on + strand, within glpGat 3558.776 kb on + strand, within glpGat 3558.791 kb on - strand, within glpGat 3558.794 kb on - strand, within glpGat 3558.816 kb on - strand, within glpGat 3558.851 kb on - strand, within glpGat 3558.855 kb on - strand, within glpGat 3558.867 kb on - strand, within glpGat 3558.867 kb on - strand, within glpGat 3558.919 kb on - strand, within glpGat 3558.921 kb on - strand, within glpGat 3558.921 kb on - strand, within glpGat 3558.927 kb on - strand, within glpGat 3558.975 kb on + strand, within glpGat 3558.991 kb on - strand, within glpGat 3559.003 kb on + strand, within glpGat 3559.041 kb on - strand, within glpGat 3559.041 kb on - strand, within glpGat 3559.049 kb on - strand, within glpGat 3559.056 kb on + strand, within glpGat 3559.056 kb on + strand, within glpGat 3559.058 kb on - strand, within glpGat 3559.058 kb on - strand, within glpGat 3559.117 kb on - strand, within glpGat 3559.127 kb on - strand, within glpGat 3559.127 kb on - strand, within glpGat 3559.129 kb on - strand, within glpGat 3559.160 kb on - strand, within glpGat 3559.180 kb on + strand, within glpGat 3559.180 kb on + strand, within glpGat 3559.185 kb on + strand, within glpGat 3559.193 kb on + strand, within glpGat 3559.223 kb on + strand, within glpGat 3559.223 kb on + strand, within glpGat 3559.234 kb on - strand, within glpGat 3559.259 kb on + strand, within glpGat 3559.262 kb on - strand, within glpGat 3559.262 kb on - strand, within glpGat 3559.325 kb on - strand, within glpGat 3559.342 kb on + strand, within glpGat 3559.350 kb on - strand, within glpGat 3559.350 kb on - strand, within glpGat 3559.350 kb on - strand, within glpGat 3559.350 kb on - strand, within glpGat 3559.370 kb on + strand, within glpGat 3559.370 kb on + strand, within glpGat 3559.383 kb on - strand, within glpGat 3559.405 kb on - strandat 3559.405 kb on - strandat 3559.419 kb on - strandat 3559.419 kb on - strandat 3559.439 kb on - strandat 3559.510 kb on + strandat 3559.510 kb on + strandat 3559.518 kb on - strandat 3559.583 kb on + strand, within glpEat 3559.584 kb on + strand, within glpEat 3559.587 kb on - strand, within glpEat 3559.592 kb on - strand, within glpEat 3559.592 kb on - strand, within glpEat 3559.603 kb on - strand, within glpEat 3559.624 kb on - strand, within glpEat 3559.661 kb on + strand, within glpEat 3559.663 kb on + strand, within glpEat 3559.663 kb on + strand, within glpEat 3559.715 kb on + strand, within glpEat 3559.719 kb on + strand, within glpEat 3559.719 kb on + strand, within glpEat 3559.735 kb on - strand, within glpEat 3559.737 kb on + strand, within glpEat 3559.741 kb on + strand, within glpEat 3559.745 kb on - strand, within glpEat 3559.822 kb on + strandat 3559.848 kb on - strandat 3559.891 kb on + strandat 3559.899 kb on - strandat 3559.922 kb on + strandat 3559.927 kb on + strandat 3559.927 kb on + strandat 3559.930 kb on - strandat 3560.077 kb on - strandat 3560.194 kb on + strand, within glpDat 3560.199 kb on + strand, within glpDat 3560.265 kb on - strand, within glpDat 3560.273 kb on + strand, within glpDat 3560.358 kb on - strand, within glpDat 3560.358 kb on - strand, within glpDat 3560.369 kb on + strand, within glpDat 3560.466 kb on + strand, within glpD

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Per-strain Table

Position Strand Gene LocusTag Fraction Glycolic Acid (C)
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3,557,673 + rtcR b3422 0.86 +0.3
3,557,709 - rtcR b3422 0.89 +0.1
3,557,709 - rtcR b3422 0.89 +1.0
3,557,833 - +2.0
3,557,849 + -1.3
3,557,849 + -3.0
3,557,881 + -0.1
3,557,884 + -2.3
3,557,908 - +0.2
3,557,908 - +0.2
3,557,948 - glpR b3423 0.10 -1.8
3,557,949 + glpR b3423 0.10 -2.0
3,557,959 + glpR b3423 0.12 -0.6
3,557,968 + glpR b3423 0.13 -1.2
3,558,019 + glpR b3423 0.20 -1.3
3,558,019 + glpR b3423 0.20 -3.2
3,558,069 + glpR b3423 0.26 -2.8
3,558,069 + glpR b3423 0.26 -1.9
3,558,101 + glpR b3423 0.30 -2.7
3,558,103 - glpR b3423 0.31 -1.7
3,558,103 - glpR b3423 0.31 -0.6
3,558,103 - glpR b3423 0.31 -1.9
3,558,104 - glpR b3423 0.31 -1.6
3,558,104 - glpR b3423 0.31 -0.7
3,558,115 + glpR b3423 0.32 -1.8
3,558,143 - glpR b3423 0.36 -1.6
3,558,156 - glpR b3423 0.38 -1.1
3,558,156 - glpR b3423 0.38 -0.4
3,558,221 - glpR b3423 0.46 -2.7
3,558,266 + glpR b3423 0.52 -1.6
3,558,301 + glpR b3423 0.57 -1.1
3,558,309 - glpR b3423 0.58 -2.0
3,558,309 - glpR b3423 0.58 -1.3
3,558,309 - glpR b3423 0.58 -1.3
3,558,309 - glpR b3423 0.58 -0.8
3,558,309 - glpR b3423 0.58 -0.9
3,558,327 + glpR b3423 0.60 -1.1
3,558,364 - glpR b3423 0.65 -0.4
3,558,364 - glpR b3423 0.65 -2.4
3,558,407 + glpR b3423 0.71 -0.3
3,558,409 + glpR b3423 0.71 -1.9
3,558,415 + glpR b3423 0.72 -1.7
3,558,523 - glpR b3423 0.86 -2.3
3,558,572 - -1.5
3,558,578 + -2.6
3,558,578 + -1.0
3,558,588 - -2.0
3,558,606 + -1.3
3,558,687 + +0.7
3,558,753 + glpG b3424 0.13 -0.8
3,558,766 + glpG b3424 0.15 +0.2
3,558,774 - glpG b3424 0.16 +1.0
3,558,776 + glpG b3424 0.16 -2.3
3,558,776 + glpG b3424 0.16 -0.2
3,558,791 - glpG b3424 0.18 +0.7
3,558,794 - glpG b3424 0.18 +0.0
3,558,816 - glpG b3424 0.21 -0.2
3,558,851 - glpG b3424 0.25 +0.0
3,558,855 - glpG b3424 0.25 -0.1
3,558,867 - glpG b3424 0.27 +0.4
3,558,867 - glpG b3424 0.27 +1.9
3,558,919 - glpG b3424 0.33 +1.1
3,558,921 - glpG b3424 0.33 +0.1
3,558,921 - glpG b3424 0.33 +1.5
3,558,927 - glpG b3424 0.34 +0.2
3,558,975 + glpG b3424 0.40 -2.2
3,558,991 - glpG b3424 0.42 -0.3
3,559,003 + glpG b3424 0.43 +1.6
3,559,041 - glpG b3424 0.48 +0.2
3,559,041 - glpG b3424 0.48 +1.0
3,559,049 - glpG b3424 0.49 +0.7
3,559,056 + glpG b3424 0.49 -1.0
3,559,056 + glpG b3424 0.49 -2.4
3,559,058 - glpG b3424 0.50 -0.1
3,559,058 - glpG b3424 0.50 +0.6
3,559,117 - glpG b3424 0.57 +0.5
3,559,127 - glpG b3424 0.58 +2.5
3,559,127 - glpG b3424 0.58 +0.7
3,559,129 - glpG b3424 0.58 +0.1
3,559,160 - glpG b3424 0.62 +1.2
3,559,180 + glpG b3424 0.64 -1.1
3,559,180 + glpG b3424 0.64 -1.1
3,559,185 + glpG b3424 0.65 -1.3
3,559,193 + glpG b3424 0.66 -1.3
3,559,223 + glpG b3424 0.70 -2.7
3,559,223 + glpG b3424 0.70 -1.6
3,559,234 - glpG b3424 0.71 +1.4
3,559,259 + glpG b3424 0.74 -1.7
3,559,262 - glpG b3424 0.74 +0.7
3,559,262 - glpG b3424 0.74 +0.9
3,559,325 - glpG b3424 0.82 -0.4
3,559,342 + glpG b3424 0.84 +0.2
3,559,350 - glpG b3424 0.85 -0.3
3,559,350 - glpG b3424 0.85 +1.3
3,559,350 - glpG b3424 0.85 +1.0
3,559,350 - glpG b3424 0.85 -0.2
3,559,370 + glpG b3424 0.87 +0.7
3,559,370 + glpG b3424 0.87 -0.3
3,559,383 - glpG b3424 0.89 +1.7
3,559,405 - +0.7
3,559,405 - -0.3
3,559,419 - -0.3
3,559,419 - +0.2
3,559,439 - +0.2
3,559,510 + -1.0
3,559,510 + -0.4
3,559,518 - -0.0
3,559,583 + glpE b3425 0.19 -0.6
3,559,584 + glpE b3425 0.20 -0.8
3,559,587 - glpE b3425 0.20 -0.2
3,559,592 - glpE b3425 0.22 -0.5
3,559,592 - glpE b3425 0.22 -1.4
3,559,603 - glpE b3425 0.25 -0.1
3,559,624 - glpE b3425 0.32 -0.8
3,559,661 + glpE b3425 0.43 -1.6
3,559,663 + glpE b3425 0.44 -0.3
3,559,663 + glpE b3425 0.44 -0.3
3,559,715 + glpE b3425 0.60 -0.0
3,559,719 + glpE b3425 0.61 +0.2
3,559,719 + glpE b3425 0.61 +0.2
3,559,735 - glpE b3425 0.66 -0.2
3,559,737 + glpE b3425 0.66 -0.1
3,559,741 + glpE b3425 0.68 -1.7
3,559,745 - glpE b3425 0.69 -1.6
3,559,822 + +3.1
3,559,848 - -0.3
3,559,891 + +1.0
3,559,899 - +1.0
3,559,922 + -0.1
3,559,927 + +1.2
3,559,927 + +0.8
3,559,930 - +1.8
3,560,077 - -0.7
3,560,194 + glpD b3426 0.10 -1.8
3,560,199 + glpD b3426 0.11 -1.7
3,560,265 - glpD b3426 0.15 +0.4
3,560,273 + glpD b3426 0.16 -0.6
3,560,358 - glpD b3426 0.21 +0.7
3,560,358 - glpD b3426 0.21 -0.3
3,560,369 + glpD b3426 0.22 +0.9
3,560,466 + glpD b3426 0.29 +0.2

Or see this region's nucleotide sequence