Strain Fitness in Escherichia coli BW25113 around b4178

Experiment: Glycolic Acid (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpurA and yjeB are separated by 204 nucleotidesyjeB and vacB are separated by 38 nucleotides b4177: purA - adenylosuccinate synthetase (NCBI), at 4,402,710 to 4,404,008 purA b4178: yjeB - predicted DNA-binding transcriptional regulator (NCBI), at 4,404,213 to 4,404,638 yjeB b4179: vacB - putative enzyme (VIMSS), at 4,404,677 to 4,407,118 vacB Position (kb) 4404 4405Strain fitness (log2 ratio) -2 -1 0 1 2at 4403.242 kb on - strand, within purAat 4403.472 kb on - strand, within purAat 4403.566 kb on - strand, within purAat 4403.574 kb on - strand, within purAat 4403.855 kb on - strand, within purAat 4403.965 kb on + strandat 4403.967 kb on - strandat 4404.050 kb on + strandat 4404.050 kb on + strandat 4404.050 kb on + strandat 4404.050 kb on + strandat 4404.070 kb on - strandat 4404.095 kb on - strandat 4404.095 kb on - strandat 4404.097 kb on + strandat 4404.097 kb on + strandat 4404.169 kb on - strandat 4404.254 kb on + strandat 4404.311 kb on + strand, within yjeBat 4404.311 kb on + strand, within yjeBat 4404.312 kb on + strand, within yjeBat 4404.312 kb on + strand, within yjeBat 4404.364 kb on - strand, within yjeBat 4404.364 kb on - strand, within yjeBat 4404.379 kb on - strand, within yjeBat 4404.379 kb on - strand, within yjeBat 4404.490 kb on - strand, within yjeBat 4404.536 kb on + strand, within yjeBat 4404.536 kb on + strand, within yjeBat 4404.536 kb on + strand, within yjeBat 4404.536 kb on + strand, within yjeBat 4404.536 kb on + strand, within yjeBat 4404.544 kb on - strand, within yjeBat 4404.544 kb on - strand, within yjeBat 4404.567 kb on + strand, within yjeBat 4404.583 kb on - strand, within yjeBat 4404.600 kb on - strandat 4404.620 kb on - strandat 4404.695 kb on + strandat 4404.695 kb on + strandat 4404.806 kb on - strandat 4404.819 kb on - strandat 4404.873 kb on - strandat 4404.898 kb on + strandat 4404.898 kb on + strandat 4404.898 kb on + strandat 4404.928 kb on + strand, within vacBat 4404.928 kb on + strand, within vacBat 4404.952 kb on + strand, within vacBat 4404.966 kb on + strand, within vacBat 4404.966 kb on + strand, within vacBat 4405.013 kb on - strand, within vacBat 4405.066 kb on - strand, within vacBat 4405.066 kb on - strand, within vacBat 4405.080 kb on - strand, within vacBat 4405.151 kb on - strand, within vacBat 4405.187 kb on + strand, within vacBat 4405.245 kb on + strand, within vacBat 4405.278 kb on - strand, within vacBat 4405.313 kb on + strand, within vacBat 4405.348 kb on + strand, within vacBat 4405.358 kb on - strand, within vacBat 4405.463 kb on - strand, within vacBat 4405.463 kb on - strand, within vacBat 4405.580 kb on + strand, within vacBat 4405.590 kb on + strand, within vacBat 4405.590 kb on + strand, within vacBat 4405.590 kb on + strand, within vacB

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Per-strain Table

Position Strand Gene LocusTag Fraction Glycolic Acid (C)
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4,403,242 - purA b4177 0.41 -1.8
4,403,472 - purA b4177 0.59 -1.9
4,403,566 - purA b4177 0.66 +0.2
4,403,574 - purA b4177 0.67 -1.2
4,403,855 - purA b4177 0.88 -1.8
4,403,965 + -0.2
4,403,967 - -2.4
4,404,050 + +0.9
4,404,050 + +1.0
4,404,050 + +0.5
4,404,050 + -0.2
4,404,070 - +0.3
4,404,095 - +0.1
4,404,095 - -0.4
4,404,097 + +1.2
4,404,097 + +2.0
4,404,169 - -1.8
4,404,254 + -1.1
4,404,311 + yjeB b4178 0.23 -2.2
4,404,311 + yjeB b4178 0.23 -1.2
4,404,312 + yjeB b4178 0.23 -1.4
4,404,312 + yjeB b4178 0.23 -1.4
4,404,364 - yjeB b4178 0.35 -1.2
4,404,364 - yjeB b4178 0.35 -0.8
4,404,379 - yjeB b4178 0.39 -0.6
4,404,379 - yjeB b4178 0.39 -1.8
4,404,490 - yjeB b4178 0.65 -0.4
4,404,536 + yjeB b4178 0.76 -0.9
4,404,536 + yjeB b4178 0.76 -0.8
4,404,536 + yjeB b4178 0.76 -2.4
4,404,536 + yjeB b4178 0.76 -0.8
4,404,536 + yjeB b4178 0.76 -0.2
4,404,544 - yjeB b4178 0.78 -2.0
4,404,544 - yjeB b4178 0.78 -0.6
4,404,567 + yjeB b4178 0.83 -1.3
4,404,583 - yjeB b4178 0.87 -2.1
4,404,600 - -1.9
4,404,620 - -1.7
4,404,695 + -2.2
4,404,695 + -1.0
4,404,806 - +0.5
4,404,819 - -1.4
4,404,873 - -0.9
4,404,898 + -0.6
4,404,898 + +0.8
4,404,898 + -2.2
4,404,928 + vacB b4179 0.10 +0.6
4,404,928 + vacB b4179 0.10 +0.9
4,404,952 + vacB b4179 0.11 -2.0
4,404,966 + vacB b4179 0.12 -2.6
4,404,966 + vacB b4179 0.12 -0.3
4,405,013 - vacB b4179 0.14 -1.7
4,405,066 - vacB b4179 0.16 -1.4
4,405,066 - vacB b4179 0.16 +0.2
4,405,080 - vacB b4179 0.17 -0.2
4,405,151 - vacB b4179 0.19 -2.0
4,405,187 + vacB b4179 0.21 -2.0
4,405,245 + vacB b4179 0.23 -1.7
4,405,278 - vacB b4179 0.25 -1.4
4,405,313 + vacB b4179 0.26 +0.2
4,405,348 + vacB b4179 0.27 -1.6
4,405,358 - vacB b4179 0.28 -1.8
4,405,463 - vacB b4179 0.32 -1.9
4,405,463 - vacB b4179 0.32 -2.0
4,405,580 + vacB b4179 0.37 -0.6
4,405,590 + vacB b4179 0.37 -0.8
4,405,590 + vacB b4179 0.37 -1.4
4,405,590 + vacB b4179 0.37 -1.7

Or see this region's nucleotide sequence