Experiment: Glycolic Acid (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mrcA and nudE are separated by 165 nucleotides nudE and yrfF are separated by 319 nucleotides
b3396: mrcA - peptidoglycan synthetase; penicillin-binding protein 1A (VIMSS), at 3,520,893 to 3,523,445
mrcA
b3397: nudE - ADP-ribose diphosphatase (NCBI), at 3,523,611 to 3,524,171
nudE
b3398: yrfF - predicted inner membrane protein (NCBI), at 3,524,491 to 3,526,626
yrfF
Position (kb)
3523
3524
3525 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3522.645 kb on + strand, within mrcA at 3522.645 kb on + strand, within mrcA at 3522.664 kb on - strand, within mrcA at 3522.664 kb on - strand, within mrcA at 3522.684 kb on + strand, within mrcA at 3522.744 kb on - strand, within mrcA at 3522.832 kb on + strand, within mrcA at 3522.859 kb on - strand, within mrcA at 3522.869 kb on + strand, within mrcA at 3522.884 kb on + strand, within mrcA at 3522.896 kb on + strand, within mrcA at 3522.911 kb on + strand, within mrcA at 3522.922 kb on - strand, within mrcA at 3522.932 kb on - strand, within mrcA at 3522.946 kb on - strand, within mrcA at 3522.949 kb on - strand, within mrcA at 3522.949 kb on - strand, within mrcA at 3522.956 kb on - strand, within mrcA at 3522.956 kb on - strand, within mrcA at 3522.978 kb on + strand, within mrcA at 3522.987 kb on + strand, within mrcA at 3522.988 kb on + strand, within mrcA at 3522.991 kb on - strand, within mrcA at 3523.005 kb on - strand, within mrcA at 3523.047 kb on + strand, within mrcA at 3523.047 kb on + strand, within mrcA at 3523.047 kb on + strand, within mrcA at 3523.057 kb on - strand, within mrcA at 3523.057 kb on - strand, within mrcA at 3523.118 kb on + strand, within mrcA at 3523.130 kb on - strand, within mrcA at 3523.178 kb on + strand, within mrcA at 3523.178 kb on + strand, within mrcA at 3523.207 kb on - strand at 3523.224 kb on - strand at 3523.238 kb on - strand at 3523.238 kb on - strand at 3523.273 kb on + strand at 3523.273 kb on + strand at 3523.275 kb on - strand at 3523.275 kb on - strand at 3523.291 kb on - strand at 3523.307 kb on + strand at 3523.322 kb on + strand at 3523.322 kb on - strand at 3523.332 kb on + strand at 3523.334 kb on - strand at 3523.335 kb on + strand at 3523.335 kb on + strand at 3523.348 kb on - strand at 3523.348 kb on - strand at 3523.352 kb on + strand at 3523.366 kb on + strand at 3523.389 kb on + strand at 3523.389 kb on + strand at 3523.389 kb on + strand at 3523.393 kb on - strand at 3523.397 kb on - strand at 3523.398 kb on - strand at 3523.398 kb on - strand at 3523.404 kb on - strand at 3523.408 kb on + strand at 3523.408 kb on + strand at 3523.518 kb on + strand at 3523.577 kb on + strand at 3523.587 kb on + strand at 3523.587 kb on + strand at 3523.593 kb on - strand at 3523.608 kb on + strand at 3523.608 kb on + strand at 3523.608 kb on + strand at 3523.608 kb on + strand at 3523.608 kb on + strand at 3523.628 kb on + strand at 3523.628 kb on + strand at 3523.635 kb on - strand at 3523.635 kb on - strand at 3523.657 kb on - strand at 3523.662 kb on + strand at 3523.663 kb on + strand at 3523.666 kb on - strand at 3523.666 kb on - strand at 3523.670 kb on - strand, within nudE at 3523.713 kb on - strand, within nudE at 3523.742 kb on + strand, within nudE at 3523.742 kb on + strand, within nudE at 3523.745 kb on - strand, within nudE at 3523.788 kb on + strand, within nudE at 3523.788 kb on + strand, within nudE at 3523.797 kb on - strand, within nudE at 3523.797 kb on - strand, within nudE at 3523.823 kb on + strand, within nudE at 3523.823 kb on + strand, within nudE at 3523.868 kb on + strand, within nudE at 3523.933 kb on - strand, within nudE at 3523.933 kb on - strand, within nudE at 3523.933 kb on - strand, within nudE at 3523.972 kb on + strand, within nudE at 3523.980 kb on - strand, within nudE at 3523.983 kb on + strand, within nudE at 3524.017 kb on + strand, within nudE at 3524.045 kb on - strand, within nudE at 3524.060 kb on - strand, within nudE at 3524.060 kb on - strand, within nudE at 3524.071 kb on + strand, within nudE at 3524.077 kb on - strand, within nudE at 3524.101 kb on + strand, within nudE at 3524.111 kb on - strand, within nudE at 3524.129 kb on - strand at 3524.177 kb on + strand at 3524.179 kb on - strand at 3524.179 kb on - strand at 3524.185 kb on - strand at 3524.185 kb on - strand at 3524.186 kb on + strand at 3524.220 kb on + strand at 3524.231 kb on + strand at 3524.231 kb on + strand at 3524.294 kb on + strand at 3524.356 kb on - strand at 3524.375 kb on - strand at 3524.379 kb on + strand at 3524.379 kb on + strand at 3524.387 kb on - strand at 3524.394 kb on + strand at 3524.394 kb on + strand at 3524.395 kb on + strand at 3524.432 kb on + strand at 3524.445 kb on - strand at 3524.460 kb on + strand at 3524.467 kb on - strand at 3524.467 kb on - strand at 3524.469 kb on + strand at 3524.521 kb on + strand at 3524.538 kb on + strand at 3524.538 kb on + strand at 3524.584 kb on + strand at 3524.590 kb on + strand at 3524.718 kb on + strand, within yrfF at 3524.733 kb on + strand, within yrfF at 3524.748 kb on + strand, within yrfF at 3524.763 kb on + strand, within yrfF at 3524.850 kb on + strand, within yrfF at 3524.895 kb on + strand, within yrfF at 3524.979 kb on + strand, within yrfF at 3524.979 kb on + strand, within yrfF
Per-strain Table
Position Strand Gene LocusTag Fraction Glycolic Acid (C) remove 3,522,645 + mrcA b3396 0.69 +0.9 3,522,645 + mrcA b3396 0.69 -0.6 3,522,664 - mrcA b3396 0.69 -0.4 3,522,664 - mrcA b3396 0.69 -0.5 3,522,684 + mrcA b3396 0.70 -0.9 3,522,744 - mrcA b3396 0.73 +1.3 3,522,832 + mrcA b3396 0.76 +0.1 3,522,859 - mrcA b3396 0.77 -1.7 3,522,869 + mrcA b3396 0.77 +0.0 3,522,884 + mrcA b3396 0.78 -0.8 3,522,896 + mrcA b3396 0.78 +0.2 3,522,911 + mrcA b3396 0.79 -1.0 3,522,922 - mrcA b3396 0.79 -0.2 3,522,932 - mrcA b3396 0.80 -3.4 3,522,946 - mrcA b3396 0.80 -0.2 3,522,949 - mrcA b3396 0.81 +1.0 3,522,949 - mrcA b3396 0.81 +0.4 3,522,956 - mrcA b3396 0.81 -0.9 3,522,956 - mrcA b3396 0.81 -0.4 3,522,978 + mrcA b3396 0.82 +0.3 3,522,987 + mrcA b3396 0.82 +0.8 3,522,988 + mrcA b3396 0.82 +0.7 3,522,991 - mrcA b3396 0.82 +0.2 3,523,005 - mrcA b3396 0.83 +0.9 3,523,047 + mrcA b3396 0.84 +2.0 3,523,047 + mrcA b3396 0.84 -0.2 3,523,047 + mrcA b3396 0.84 -0.2 3,523,057 - mrcA b3396 0.85 -2.7 3,523,057 - mrcA b3396 0.85 -0.1 3,523,118 + mrcA b3396 0.87 -0.5 3,523,130 - mrcA b3396 0.88 -0.2 3,523,178 + mrcA b3396 0.90 +2.2 3,523,178 + mrcA b3396 0.90 +0.4 3,523,207 - -0.4 3,523,224 - -0.2 3,523,238 - +2.1 3,523,238 - -0.7 3,523,273 + +0.1 3,523,273 + +0.6 3,523,275 - +0.4 3,523,275 - +0.2 3,523,291 - -1.2 3,523,307 + +0.6 3,523,322 + +0.0 3,523,322 - -0.7 3,523,332 + +0.3 3,523,334 - -0.6 3,523,335 + +0.1 3,523,335 + +0.2 3,523,348 - +0.0 3,523,348 - +0.6 3,523,352 + -0.5 3,523,366 + -2.5 3,523,389 + +0.0 3,523,389 + -0.1 3,523,389 + -0.5 3,523,393 - -0.2 3,523,397 - -0.9 3,523,398 - -0.9 3,523,398 - -0.6 3,523,404 - -0.8 3,523,408 + +0.2 3,523,408 + -0.5 3,523,518 + +0.2 3,523,577 + +1.5 3,523,587 + -0.4 3,523,587 + -0.4 3,523,593 - +0.3 3,523,608 + +0.8 3,523,608 + +0.9 3,523,608 + +0.0 3,523,608 + +1.5 3,523,608 + +1.4 3,523,628 + -0.0 3,523,628 + +0.8 3,523,635 - +0.2 3,523,635 - +2.0 3,523,657 - +1.1 3,523,662 + +0.9 3,523,663 + +1.7 3,523,666 - +1.6 3,523,666 - +1.5 3,523,670 - nudE b3397 0.11 -0.0 3,523,713 - nudE b3397 0.18 +1.4 3,523,742 + nudE b3397 0.23 +0.6 3,523,742 + nudE b3397 0.23 -0.1 3,523,745 - nudE b3397 0.24 +1.4 3,523,788 + nudE b3397 0.32 +1.5 3,523,788 + nudE b3397 0.32 +1.4 3,523,797 - nudE b3397 0.33 +1.7 3,523,797 - nudE b3397 0.33 +1.8 3,523,823 + nudE b3397 0.38 +1.3 3,523,823 + nudE b3397 0.38 +1.1 3,523,868 + nudE b3397 0.46 +0.9 3,523,933 - nudE b3397 0.57 +0.3 3,523,933 - nudE b3397 0.57 +1.1 3,523,933 - nudE b3397 0.57 +1.8 3,523,972 + nudE b3397 0.64 +1.5 3,523,980 - nudE b3397 0.66 +0.5 3,523,983 + nudE b3397 0.66 +0.5 3,524,017 + nudE b3397 0.72 +1.7 3,524,045 - nudE b3397 0.77 +1.5 3,524,060 - nudE b3397 0.80 +2.0 3,524,060 - nudE b3397 0.80 +1.4 3,524,071 + nudE b3397 0.82 +1.5 3,524,077 - nudE b3397 0.83 +1.2 3,524,101 + nudE b3397 0.87 +1.4 3,524,111 - nudE b3397 0.89 +0.6 3,524,129 - +1.5 3,524,177 + +0.1 3,524,179 - -1.6 3,524,179 - +1.5 3,524,185 - -1.5 3,524,185 - -1.9 3,524,186 + +0.9 3,524,220 + +1.2 3,524,231 + +1.1 3,524,231 + +0.4 3,524,294 + +0.2 3,524,356 - -0.1 3,524,375 - -1.5 3,524,379 + -2.0 3,524,379 + +0.0 3,524,387 - +0.2 3,524,394 + -0.4 3,524,394 + -0.5 3,524,395 + -0.7 3,524,432 + +0.1 3,524,445 - -0.3 3,524,460 + +0.5 3,524,467 - -1.8 3,524,467 - -0.4 3,524,469 + -1.4 3,524,521 + -1.4 3,524,538 + -3.6 3,524,538 + +0.0 3,524,584 + -0.6 3,524,590 + -0.7 3,524,718 + yrfF b3398 0.11 -1.6 3,524,733 + yrfF b3398 0.11 -0.3 3,524,748 + yrfF b3398 0.12 -1.1 3,524,763 + yrfF b3398 0.13 -2.7 3,524,850 + yrfF b3398 0.17 -3.2 3,524,895 + yrfF b3398 0.19 -1.7 3,524,979 + yrfF b3398 0.23 -2.4 3,524,979 + yrfF b3398 0.23 -1.3
Or see this region's nucleotide sequence