Strain Fitness in Escherichia coli BW25113 around b2753

Experiment: Glycolic Acid (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntcysD and iap are separated by 251 nucleotidesiap and 11403151 are separated by 82 nucleotides11403151 and 11545514 overlap by 29 nucleotides11545514 and 11687906 overlap by 29 nucleotides11687906 and 11403152 are separated by 0 nucleotides11403152 and 11545515 overlap by 32 nucleotides11545515 and 11687907 overlap by 32 nucleotides11687907 and 11403153 are separated by 0 nucleotides11403153 and 11545516 overlap by 29 nucleotides11545516 and 11687908 overlap by 29 nucleotides11687908 and 11403154 are separated by 0 nucleotides11403154 and 11545517 overlap by 32 nucleotides11545517 and 11687909 overlap by 32 nucleotides11687909 and 11403155 are separated by 0 nucleotides11403155 and 11545518 overlap by 29 nucleotides11545518 and 11687910 overlap by 29 nucleotides11687910 and 11403156 are separated by 0 nucleotides11403156 and 11545519 overlap by 32 nucleotides11545519 and 11687911 overlap by 32 nucleotides11687911 and 11403157 are separated by 0 nucleotides11403157 and 11545520 overlap by 29 nucleotides11545520 and 11687912 overlap by 29 nucleotides11687912 and 11403158 are separated by 0 nucleotides11403158 and 11545521 overlap by 32 nucleotides11545521 and 11687913 overlap by 32 nucleotides11687913 and 11403159 are separated by 0 nucleotides11403159 and 11545522 overlap by 29 nucleotides11545522 and 11687914 overlap by 29 nucleotides11687914 and 11403160 are separated by 0 nucleotides11403160 and 11545523 overlap by 32 nucleotides11545523 and 11687915 overlap by 32 nucleotides11687915 and 11403161 are separated by 0 nucleotides11403161 and 11545524 overlap by 29 nucleotides11545524 and 11687916 overlap by 29 nucleotides11687916 and 11403162 are separated by 0 nucleotides11403162 and 11545525 overlap by 32 nucleotides11545525 and 11687917 overlap by 32 nucleotides11687917 and 11403163 are separated by 0 nucleotides11403163 and 11545526 overlap by 29 nucleotides11545526 and 11687918 overlap by 29 nucleotides11687918 and 11403164 are separated by 0 nucleotides11403164 and 11545527 overlap by 32 nucleotides11545527 and 11687919 overlap by 32 nucleotides11687919 and 11403165 are separated by 0 nucleotides11403165 and 11545528 overlap by 29 nucleotides11545528 and 11687920 overlap by 29 nucleotides11687920 and 11403166 are separated by 0 nucleotides11403166 and 11545529 overlap by 33 nucleotides11545529 and 11687921 overlap by 33 nucleotides11687921 and 11403167 are separated by 0 nucleotides11403167 and 11545530 overlap by 29 nucleotides11545530 and 11687922 overlap by 29 nucleotides11687922 and 11403168 are separated by 0 nucleotides11403168 and 11545531 overlap by 33 nucleotides11545531 and 11687923 overlap by 33 nucleotides11687923 and 11403169 are separated by 0 nucleotides11403169 and 11545532 overlap by 29 nucleotides11545532 and 11687924 overlap by 29 nucleotides11687924 and 11403170 are separated by 0 nucleotides11403170 and 11545533 overlap by 32 nucleotides11545533 and 11687925 overlap by 32 nucleotides11687925 and 11403171 are separated by 0 nucleotides11403171 and 11545534 overlap by 29 nucleotides11545534 and 11687926 overlap by 29 nucleotides11687926 and 11403172 are separated by 0 nucleotides11403172 and 11545535 overlap by 32 nucleotides11545535 and 11687927 overlap by 32 nucleotides11687927 and 11403173 are separated by 0 nucleotides11403173 and 11545536 overlap by 29 nucleotides11545536 and 11687928 overlap by 29 nucleotides11687928 and 11403174 are separated by 0 nucleotides11403174 and 11545537 overlap by 32 nucleotides11545537 and 11687929 overlap by 32 nucleotides11687929 and 11403175 are separated by 0 nucleotides11403175 and 11545538 overlap by 29 nucleotides11545538 and 11687930 overlap by 29 nucleotides11687930 and ygbF are separated by 105 nucleotides b2752: cysD - sulfate adenylyltransferase subunit 2 (NCBI), at 2,873,443 to 2,874,351 cysD b2753: iap - aminopeptidase in alkaline phosphatase isozyme conversion (NCBI), at 2,874,603 to 2,875,640 iap 11403151 - no description, at 2,875,723 to 2,875,751 11403151 11545514 - no description, at 2,875,723 to 2,875,751 11545514 11687906 - no description, at 2,875,723 to 2,875,751 11687906 11403152 - no description, at 2,875,752 to 2,875,783 11403152 11545515 - no description, at 2,875,752 to 2,875,783 11545515 11687907 - no description, at 2,875,752 to 2,875,783 11687907 11403153 - no description, at 2,875,784 to 2,875,812 11403153 11545516 - no description, at 2,875,784 to 2,875,812 11545516 11687908 - no description, at 2,875,784 to 2,875,812 11687908 11403154 - no description, at 2,875,813 to 2,875,844 11403154 11545517 - no description, at 2,875,813 to 2,875,844 11545517 11687909 - no description, at 2,875,813 to 2,875,844 11687909 11403155 - no description, at 2,875,845 to 2,875,873 11403155 11545518 - no description, at 2,875,845 to 2,875,873 11545518 11687910 - no description, at 2,875,845 to 2,875,873 11687910 11403156 - no description, at 2,875,874 to 2,875,905 11403156 11545519 - no description, at 2,875,874 to 2,875,905 11545519 11687911 - no description, at 2,875,874 to 2,875,905 11687911 11403157 - no description, at 2,875,906 to 2,875,934 11403157 11545520 - no description, at 2,875,906 to 2,875,934 11545520 11687912 - no description, at 2,875,906 to 2,875,934 11687912 11403158 - no description, at 2,875,935 to 2,875,966 11403158 11545521 - no description, at 2,875,935 to 2,875,966 11545521 11687913 - no description, at 2,875,935 to 2,875,966 11687913 11403159 - no description, at 2,875,967 to 2,875,995 11403159 11545522 - no description, at 2,875,967 to 2,875,995 11545522 11687914 - no description, at 2,875,967 to 2,875,995 11687914 11403160 - no description, at 2,875,996 to 2,876,027 11403160 11545523 - no description, at 2,875,996 to 2,876,027 11545523 11687915 - no description, at 2,875,996 to 2,876,027 11687915 11403161 - no description, at 2,876,028 to 2,876,056 11403161 11545524 - no description, at 2,876,028 to 2,876,056 11545524 11687916 - no description, at 2,876,028 to 2,876,056 11687916 11403162 - no description, at 2,876,057 to 2,876,088 11403162 11545525 - no description, at 2,876,057 to 2,876,088 11545525 11687917 - no description, at 2,876,057 to 2,876,088 11687917 11403163 - no description, at 2,876,089 to 2,876,117 11403163 11545526 - no description, at 2,876,089 to 2,876,117 11545526 11687918 - no description, at 2,876,089 to 2,876,117 11687918 11403164 - no description, at 2,876,118 to 2,876,149 11403164 11545527 - no description, at 2,876,118 to 2,876,149 11545527 11687919 - no description, at 2,876,118 to 2,876,149 11687919 11403165 - no description, at 2,876,150 to 2,876,178 11403165 11545528 - no description, at 2,876,150 to 2,876,178 11545528 11687920 - no description, at 2,876,150 to 2,876,178 11687920 11403166 - no description, at 2,876,179 to 2,876,211 11403166 11545529 - no description, at 2,876,179 to 2,876,211 11545529 11687921 - no description, at 2,876,179 to 2,876,211 11687921 11403167 - no description, at 2,876,212 to 2,876,240 11403167 11545530 - no description, at 2,876,212 to 2,876,240 11545530 11687922 - no description, at 2,876,212 to 2,876,240 11687922 11403168 - no description, at 2,876,241 to 2,876,273 11403168 11545531 - no description, at 2,876,241 to 2,876,273 11545531 11687923 - no description, at 2,876,241 to 2,876,273 11687923 11403169 - no description, at 2,876,274 to 2,876,302 11403169 11545532 - no description, at 2,876,274 to 2,876,302 11545532 11687924 - no description, at 2,876,274 to 2,876,302 11687924 11403170 - no description, at 2,876,303 to 2,876,334 11403170 11545533 - no description, at 2,876,303 to 2,876,334 11545533 11687925 - no description, at 2,876,303 to 2,876,334 11687925 11403171 - no description, at 2,876,335 to 2,876,363 11403171 11545534 - no description, at 2,876,335 to 2,876,363 11545534 11687926 - no description, at 2,876,335 to 2,876,363 11687926 11403172 - no description, at 2,876,364 to 2,876,395 11403172 11545535 - no description, at 2,876,364 to 2,876,395 11545535 11687927 - no description, at 2,876,364 to 2,876,395 11687927 11403173 - no description, at 2,876,396 to 2,876,424 11403173 11545536 - no description, at 2,876,396 to 2,876,424 11545536 11687928 - no description, at 2,876,396 to 2,876,424 11687928 11403174 - no description, at 2,876,425 to 2,876,456 11403174 11545537 - no description, at 2,876,425 to 2,876,456 11545537 11687929 - no description, at 2,876,425 to 2,876,456 11687929 11403175 - no description, at 2,876,457 to 2,876,485 11403175 11545538 - no description, at 2,876,457 to 2,876,485 11545538 11687930 - no description, at 2,876,457 to 2,876,485 11687930 b2754: ygbF - orf, hypothetical protein (VIMSS), at 2,876,591 to 2,876,875 ygbF Position (kb) 2874 2875 2876Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 2873.614 kb on + strand, within cysDat 2873.773 kb on + strand, within cysDat 2873.773 kb on + strand, within cysDat 2873.837 kb on + strand, within cysDat 2873.951 kb on - strand, within cysDat 2873.951 kb on - strand, within cysDat 2873.953 kb on + strand, within cysDat 2874.048 kb on + strand, within cysDat 2874.225 kb on - strand, within cysDat 2874.306 kb on + strandat 2874.325 kb on - strandat 2874.330 kb on - strandat 2874.343 kb on - strandat 2874.362 kb on + strandat 2874.362 kb on + strandat 2874.367 kb on - strandat 2874.394 kb on - strandat 2874.394 kb on - strandat 2874.399 kb on + strandat 2874.399 kb on + strandat 2874.399 kb on + strandat 2874.399 kb on + strandat 2874.407 kb on - strandat 2874.407 kb on - strandat 2874.407 kb on - strandat 2874.435 kb on + strandat 2874.459 kb on + strandat 2874.499 kb on - strandat 2874.500 kb on + strandat 2874.561 kb on - strandat 2874.561 kb on - strandat 2874.562 kb on - strandat 2874.586 kb on + strandat 2874.620 kb on + strandat 2874.622 kb on + strandat 2874.623 kb on + strandat 2874.623 kb on + strandat 2874.623 kb on + strandat 2874.623 kb on + strandat 2874.628 kb on + strandat 2874.628 kb on - strandat 2874.631 kb on - strandat 2874.631 kb on - strandat 2874.638 kb on + strandat 2874.641 kb on - strandat 2874.641 kb on - strandat 2874.641 kb on - strandat 2874.658 kb on - strandat 2874.666 kb on + strandat 2874.706 kb on - strandat 2874.709 kb on + strand, within iapat 2874.747 kb on + strand, within iapat 2874.747 kb on + strand, within iapat 2874.751 kb on - strand, within iapat 2874.755 kb on - strand, within iapat 2874.755 kb on - strand, within iapat 2874.764 kb on + strand, within iapat 2874.764 kb on + strand, within iapat 2874.784 kb on - strand, within iapat 2874.841 kb on + strand, within iapat 2874.841 kb on + strand, within iapat 2874.845 kb on + strand, within iapat 2874.845 kb on + strand, within iapat 2874.853 kb on - strand, within iapat 2874.866 kb on + strand, within iapat 2874.878 kb on + strand, within iapat 2874.903 kb on - strand, within iapat 2874.915 kb on - strand, within iapat 2874.937 kb on + strand, within iapat 2874.937 kb on + strand, within iapat 2874.946 kb on - strand, within iapat 2874.961 kb on - strand, within iapat 2874.968 kb on - strand, within iapat 2874.969 kb on + strand, within iapat 2875.012 kb on + strand, within iapat 2875.012 kb on + strand, within iapat 2875.020 kb on - strand, within iapat 2875.021 kb on + strand, within iapat 2875.026 kb on + strand, within iapat 2875.026 kb on + strand, within iapat 2875.041 kb on + strand, within iapat 2875.053 kb on + strand, within iapat 2875.081 kb on - strand, within iapat 2875.094 kb on + strand, within iapat 2875.094 kb on + strand, within iapat 2875.097 kb on - strand, within iapat 2875.105 kb on + strand, within iapat 2875.146 kb on + strand, within iapat 2875.189 kb on + strand, within iapat 2875.214 kb on - strand, within iapat 2875.224 kb on - strandat 2875.321 kb on + strand, within iapat 2875.321 kb on + strand, within iapat 2875.321 kb on + strand, within iapat 2875.321 kb on + strand, within iapat 2875.321 kb on + strand, within iapat 2875.379 kb on - strand, within iapat 2875.384 kb on - strand, within iapat 2875.409 kb on + strand, within iapat 2875.519 kb on + strand, within iapat 2875.519 kb on + strand, within iapat 2875.519 kb on + strand, within iapat 2875.525 kb on + strand, within iapat 2875.541 kb on + strandat 2875.679 kb on - strandat 2875.746 kb on - strandat 2875.746 kb on - strandat 2875.855 kb on + strandat 2875.902 kb on - strandat 2875.962 kb on - strandat 2875.962 kb on - strandat 2876.366 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Glycolic Acid (C)
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2,873,614 + cysD b2752 0.19 -3.8
2,873,773 + cysD b2752 0.36 -2.8
2,873,773 + cysD b2752 0.36 -1.3
2,873,837 + cysD b2752 0.43 -3.7
2,873,951 - cysD b2752 0.56 -2.2
2,873,951 - cysD b2752 0.56 -2.0
2,873,953 + cysD b2752 0.56 -2.8
2,874,048 + cysD b2752 0.67 -4.0
2,874,225 - cysD b2752 0.86 -2.1
2,874,306 + -1.9
2,874,325 - -2.9
2,874,330 - -3.1
2,874,343 - -1.5
2,874,362 + -1.6
2,874,362 + -2.8
2,874,367 - -3.5
2,874,394 - -1.4
2,874,394 - +0.6
2,874,399 + -2.2
2,874,399 + -2.4
2,874,399 + -2.3
2,874,399 + -3.3
2,874,407 - -1.5
2,874,407 - -0.1
2,874,407 - +0.8
2,874,435 + -0.6
2,874,459 + -1.4
2,874,499 - -1.1
2,874,500 + -3.1
2,874,561 - -1.1
2,874,561 - -0.9
2,874,562 - +0.6
2,874,586 + -1.2
2,874,620 + +0.1
2,874,622 + -1.7
2,874,623 + -0.1
2,874,623 + -0.1
2,874,623 + -0.0
2,874,623 + -0.4
2,874,628 + -1.2
2,874,628 - -0.4
2,874,631 - -1.2
2,874,631 - -0.9
2,874,638 + -0.9
2,874,641 - -0.2
2,874,641 - +0.2
2,874,641 - +1.6
2,874,658 - -0.0
2,874,666 + -1.3
2,874,706 - -0.3
2,874,709 + iap b2753 0.10 -2.0
2,874,747 + iap b2753 0.14 +0.5
2,874,747 + iap b2753 0.14 +0.9
2,874,751 - iap b2753 0.14 +0.5
2,874,755 - iap b2753 0.15 +0.1
2,874,755 - iap b2753 0.15 +0.8
2,874,764 + iap b2753 0.16 +0.6
2,874,764 + iap b2753 0.16 -0.1
2,874,784 - iap b2753 0.17 -0.7
2,874,841 + iap b2753 0.23 -3.3
2,874,841 + iap b2753 0.23 -1.3
2,874,845 + iap b2753 0.23 -1.6
2,874,845 + iap b2753 0.23 +0.0
2,874,853 - iap b2753 0.24 +0.4
2,874,866 + iap b2753 0.25 -1.0
2,874,878 + iap b2753 0.26 -1.9
2,874,903 - iap b2753 0.29 +0.2
2,874,915 - iap b2753 0.30 -2.1
2,874,937 + iap b2753 0.32 -0.5
2,874,937 + iap b2753 0.32 -0.0
2,874,946 - iap b2753 0.33 -0.9
2,874,961 - iap b2753 0.34 -0.7
2,874,968 - iap b2753 0.35 -1.5
2,874,969 + iap b2753 0.35 +0.3
2,875,012 + iap b2753 0.39 -0.3
2,875,012 + iap b2753 0.39 -1.0
2,875,020 - iap b2753 0.40 -0.1
2,875,021 + iap b2753 0.40 +0.9
2,875,026 + iap b2753 0.41 -0.4
2,875,026 + iap b2753 0.41 +0.0
2,875,041 + iap b2753 0.42 +0.1
2,875,053 + iap b2753 0.43 -0.1
2,875,081 - iap b2753 0.46 -2.7
2,875,094 + iap b2753 0.47 -0.2
2,875,094 + iap b2753 0.47 -1.1
2,875,097 - iap b2753 0.48 -0.1
2,875,105 + iap b2753 0.48 +0.1
2,875,146 + iap b2753 0.52 +0.3
2,875,189 + iap b2753 0.56 +0.2
2,875,214 - iap b2753 0.59 -1.2
2,875,224 - +1.0
2,875,321 + iap b2753 0.69 -0.5
2,875,321 + iap b2753 0.69 -0.2
2,875,321 + iap b2753 0.69 -0.8
2,875,321 + iap b2753 0.69 +0.2
2,875,321 + iap b2753 0.69 -0.7
2,875,379 - iap b2753 0.75 -1.1
2,875,384 - iap b2753 0.75 -0.4
2,875,409 + iap b2753 0.78 -1.0
2,875,519 + iap b2753 0.88 -1.4
2,875,519 + iap b2753 0.88 +0.6
2,875,519 + iap b2753 0.88 -0.1
2,875,525 + iap b2753 0.89 -1.0
2,875,541 + -0.3
2,875,679 - +0.1
2,875,746 - +1.6
2,875,746 - +0.9
2,875,855 + +0.1
2,875,902 - +0.3
2,875,962 - +1.0
2,875,962 - +1.6
2,876,366 - -0.8

Or see this region's nucleotide sequence