Strain Fitness in Escherichia coli BW25113 around b2478

Experiment: Glycolic Acid (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntnlpB and dapA are separated by 16 nucleotidesdapA and gcvR are separated by 145 nucleotidesgcvR and bcp overlap by 1 nucleotides b2477: nlpB - lipoprotein-34 (VIMSS), at 2,595,853 to 2,596,887 nlpB b2478: dapA - dihydrodipicolinate synthase (NCBI), at 2,596,904 to 2,597,782 dapA b2479: gcvR - transcriptional regulation of gcv operon (VIMSS), at 2,597,928 to 2,598,500 gcvR b2480: bcp - thioredoxin-dependent thiol peroxidase (NCBI), at 2,598,500 to 2,598,970 bcp Position (kb) 2596 2597 2598Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2595.937 kb on + strandat 2595.937 kb on + strandat 2595.944 kb on - strandat 2595.944 kb on - strandat 2595.991 kb on + strand, within nlpBat 2595.991 kb on + strand, within nlpBat 2596.206 kb on + strand, within nlpBat 2596.295 kb on - strand, within nlpBat 2596.349 kb on - strand, within nlpBat 2596.439 kb on - strand, within nlpBat 2596.441 kb on + strand, within nlpBat 2596.792 kb on + strandat 2596.792 kb on + strandat 2596.883 kb on - strandat 2596.883 kb on - strandat 2597.909 kb on + strandat 2597.917 kb on - strandat 2597.917 kb on - strandat 2597.962 kb on - strandat 2597.988 kb on - strand, within gcvRat 2598.033 kb on + strand, within gcvRat 2598.054 kb on + strand, within gcvRat 2598.128 kb on - strand, within gcvRat 2598.129 kb on - strand, within gcvRat 2598.129 kb on - strand, within gcvRat 2598.132 kb on + strand, within gcvRat 2598.132 kb on + strand, within gcvRat 2598.139 kb on - strand, within gcvRat 2598.142 kb on + strand, within gcvRat 2598.212 kb on + strand, within gcvRat 2598.282 kb on + strand, within gcvRat 2598.285 kb on + strand, within gcvRat 2598.303 kb on - strand, within gcvRat 2598.303 kb on - strand, within gcvRat 2598.346 kb on - strand, within gcvRat 2598.400 kb on - strand, within gcvRat 2598.400 kb on - strand, within gcvRat 2598.418 kb on - strand, within gcvRat 2598.427 kb on - strand, within gcvRat 2598.513 kb on + strandat 2598.621 kb on + strand, within bcpat 2598.621 kb on + strand, within bcpat 2598.628 kb on - strand, within bcpat 2598.639 kb on - strand, within bcpat 2598.644 kb on - strand, within bcpat 2598.714 kb on - strand, within bcpat 2598.757 kb on + strand, within bcp

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Per-strain Table

Position Strand Gene LocusTag Fraction Glycolic Acid (C)
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2,595,937 + +0.1
2,595,937 + +0.9
2,595,944 - +0.8
2,595,944 - +2.6
2,595,991 + nlpB b2477 0.13 -0.8
2,595,991 + nlpB b2477 0.13 -1.2
2,596,206 + nlpB b2477 0.34 -0.2
2,596,295 - nlpB b2477 0.43 +0.1
2,596,349 - nlpB b2477 0.48 +0.2
2,596,439 - nlpB b2477 0.57 -1.0
2,596,441 + nlpB b2477 0.57 -0.9
2,596,792 + -0.0
2,596,792 + -1.6
2,596,883 - +1.2
2,596,883 - -0.0
2,597,909 + +0.2
2,597,917 - -1.1
2,597,917 - -1.4
2,597,962 - -2.1
2,597,988 - gcvR b2479 0.10 -1.9
2,598,033 + gcvR b2479 0.18 -0.6
2,598,054 + gcvR b2479 0.22 +0.6
2,598,128 - gcvR b2479 0.35 +0.6
2,598,129 - gcvR b2479 0.35 -0.4
2,598,129 - gcvR b2479 0.35 +0.6
2,598,132 + gcvR b2479 0.36 -0.8
2,598,132 + gcvR b2479 0.36 -1.2
2,598,139 - gcvR b2479 0.37 -0.7
2,598,142 + gcvR b2479 0.37 -1.2
2,598,212 + gcvR b2479 0.50 +0.6
2,598,282 + gcvR b2479 0.62 -2.8
2,598,285 + gcvR b2479 0.62 -0.0
2,598,303 - gcvR b2479 0.65 -0.0
2,598,303 - gcvR b2479 0.65 -0.2
2,598,346 - gcvR b2479 0.73 -0.5
2,598,400 - gcvR b2479 0.82 -0.9
2,598,400 - gcvR b2479 0.82 -0.6
2,598,418 - gcvR b2479 0.86 -1.2
2,598,427 - gcvR b2479 0.87 -3.2
2,598,513 + +0.7
2,598,621 + bcp b2480 0.26 +0.7
2,598,621 + bcp b2480 0.26 +0.6
2,598,628 - bcp b2480 0.27 +0.3
2,598,639 - bcp b2480 0.30 +0.7
2,598,644 - bcp b2480 0.31 +0.5
2,598,714 - bcp b2480 0.45 +1.3
2,598,757 + bcp b2480 0.55 +0.3

Or see this region's nucleotide sequence