Experiment: Glycolic Acid (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt eutT and eutQ overlap by 4 nucleotides eutQ and eutP overlap by 26 nucleotides eutP and b2462 are separated by 12 nucleotides b2462 and maeB are separated by 292 nucleotides
b2459: eutT - predicted cobalamin adenosyltransferase in ethanolamine utilization (NCBI), at 2,571,524 to 2,572,327
eutT
b2460: eutQ - hypothetical protein (NCBI), at 2,572,324 to 2,573,025
eutQ
b2461: eutP - conserved protein with nucleoside triphosphate hydrolase domain (NCBI), at 2,573,000 to 2,573,479
eutP
b2462: b2462 - orf, hypothetical protein (VIMSS), at 2,573,492 to 2,573,827
b2462
b2463: maeB - malic enzyme (NCBI), at 2,574,120 to 2,576,399
maeB
Position (kb)
2572
2573
2574 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2572.113 kb on + strand, within eutT at 2572.134 kb on + strand, within eutT at 2572.134 kb on + strand, within eutT at 2572.153 kb on - strand, within eutT at 2572.405 kb on + strand, within eutQ at 2572.471 kb on - strand, within eutQ at 2572.471 kb on - strand, within eutQ at 2572.477 kb on + strand, within eutQ at 2572.539 kb on + strand, within eutQ at 2572.539 kb on + strand, within eutQ at 2572.539 kb on + strand, within eutQ at 2572.539 kb on + strand, within eutQ at 2572.584 kb on - strand, within eutQ at 2573.126 kb on + strand, within eutP at 2573.186 kb on - strand, within eutP at 2573.186 kb on - strand, within eutP at 2573.222 kb on - strand, within eutP at 2573.225 kb on + strand, within eutP at 2573.225 kb on + strand, within eutP at 2573.237 kb on - strand, within eutP at 2573.260 kb on + strand, within eutP at 2573.260 kb on + strand, within eutP at 2573.278 kb on + strand, within eutP at 2573.278 kb on + strand, within eutP at 2573.279 kb on - strand, within eutP at 2573.281 kb on + strand, within eutP at 2573.281 kb on + strand, within eutP at 2573.281 kb on + strand, within eutP at 2573.281 kb on + strand, within eutP at 2573.281 kb on + strand, within eutP at 2573.281 kb on - strand, within eutP at 2573.281 kb on - strand, within eutP at 2573.282 kb on + strand, within eutP at 2573.282 kb on + strand, within eutP at 2573.283 kb on + strand, within eutP at 2573.283 kb on + strand, within eutP at 2573.283 kb on + strand, within eutP at 2573.284 kb on - strand, within eutP at 2573.286 kb on - strand, within eutP at 2573.291 kb on - strand, within eutP at 2573.291 kb on - strand, within eutP at 2573.304 kb on + strand, within eutP at 2573.304 kb on + strand, within eutP at 2573.304 kb on + strand, within eutP at 2573.312 kb on - strand, within eutP at 2573.315 kb on + strand, within eutP at 2573.355 kb on - strand, within eutP at 2573.356 kb on - strand, within eutP at 2573.399 kb on - strand, within eutP at 2573.399 kb on - strand, within eutP at 2573.459 kb on + strand at 2573.459 kb on - strand at 2573.459 kb on - strand at 2573.459 kb on - strand at 2573.496 kb on - strand at 2573.496 kb on - strand at 2573.496 kb on - strand at 2573.497 kb on - strand at 2573.550 kb on + strand, within b2462 at 2573.560 kb on - strand, within b2462 at 2573.562 kb on - strand, within b2462 at 2573.562 kb on - strand, within b2462 at 2573.562 kb on - strand, within b2462 at 2573.572 kb on - strand, within b2462 at 2573.572 kb on - strand, within b2462 at 2573.581 kb on - strand, within b2462 at 2573.581 kb on - strand, within b2462 at 2573.604 kb on - strand, within b2462 at 2573.606 kb on + strand, within b2462 at 2573.751 kb on - strand, within b2462 at 2573.774 kb on - strand, within b2462 at 2573.774 kb on - strand, within b2462 at 2573.775 kb on + strand, within b2462 at 2573.783 kb on - strand, within b2462 at 2573.784 kb on - strand, within b2462 at 2573.792 kb on - strand, within b2462 at 2573.818 kb on + strand at 2573.830 kb on + strand at 2573.830 kb on + strand at 2573.838 kb on - strand at 2573.838 kb on - strand at 2573.886 kb on + strand at 2573.928 kb on - strand at 2573.933 kb on - strand at 2573.963 kb on + strand at 2573.963 kb on + strand at 2574.083 kb on - strand at 2574.103 kb on - strand at 2574.133 kb on - strand at 2574.145 kb on + strand at 2574.148 kb on - strand at 2574.152 kb on - strand at 2574.158 kb on + strand at 2574.158 kb on + strand at 2574.170 kb on + strand at 2574.173 kb on - strand at 2574.187 kb on - strand at 2574.187 kb on - strand at 2574.190 kb on - strand at 2574.202 kb on - strand at 2574.216 kb on + strand at 2574.225 kb on + strand at 2574.233 kb on - strand at 2574.378 kb on - strand, within maeB at 2574.380 kb on + strand, within maeB
Per-strain Table
Position Strand Gene LocusTag Fraction Glycolic Acid (C) remove 2,572,113 + eutT b2459 0.73 -0.3 2,572,134 + eutT b2459 0.76 -1.0 2,572,134 + eutT b2459 0.76 -1.4 2,572,153 - eutT b2459 0.78 +0.5 2,572,405 + eutQ b2460 0.12 -0.0 2,572,471 - eutQ b2460 0.21 -1.6 2,572,471 - eutQ b2460 0.21 -0.1 2,572,477 + eutQ b2460 0.22 +0.4 2,572,539 + eutQ b2460 0.31 +1.0 2,572,539 + eutQ b2460 0.31 +0.1 2,572,539 + eutQ b2460 0.31 -0.2 2,572,539 + eutQ b2460 0.31 -0.0 2,572,584 - eutQ b2460 0.37 +0.2 2,573,126 + eutP b2461 0.26 -0.0 2,573,186 - eutP b2461 0.39 -0.6 2,573,186 - eutP b2461 0.39 -1.1 2,573,222 - eutP b2461 0.46 +1.0 2,573,225 + eutP b2461 0.47 -1.0 2,573,225 + eutP b2461 0.47 -0.0 2,573,237 - eutP b2461 0.49 +0.2 2,573,260 + eutP b2461 0.54 -0.7 2,573,260 + eutP b2461 0.54 -0.7 2,573,278 + eutP b2461 0.58 -0.4 2,573,278 + eutP b2461 0.58 +0.6 2,573,279 - eutP b2461 0.58 +0.7 2,573,281 + eutP b2461 0.59 +0.6 2,573,281 + eutP b2461 0.59 -0.3 2,573,281 + eutP b2461 0.59 +0.4 2,573,281 + eutP b2461 0.59 -0.3 2,573,281 + eutP b2461 0.59 +0.3 2,573,281 - eutP b2461 0.59 +0.6 2,573,281 - eutP b2461 0.59 -1.0 2,573,282 + eutP b2461 0.59 -0.4 2,573,282 + eutP b2461 0.59 +0.4 2,573,283 + eutP b2461 0.59 +0.3 2,573,283 + eutP b2461 0.59 -0.1 2,573,283 + eutP b2461 0.59 +0.4 2,573,284 - eutP b2461 0.59 -0.1 2,573,286 - eutP b2461 0.60 +0.5 2,573,291 - eutP b2461 0.61 +0.6 2,573,291 - eutP b2461 0.61 -0.0 2,573,304 + eutP b2461 0.63 -0.7 2,573,304 + eutP b2461 0.63 -0.4 2,573,304 + eutP b2461 0.63 -0.1 2,573,312 - eutP b2461 0.65 +0.2 2,573,315 + eutP b2461 0.66 +0.2 2,573,355 - eutP b2461 0.74 +0.0 2,573,356 - eutP b2461 0.74 +0.1 2,573,399 - eutP b2461 0.83 +0.9 2,573,399 - eutP b2461 0.83 +0.3 2,573,459 + +0.4 2,573,459 - +0.5 2,573,459 - +0.6 2,573,459 - -0.4 2,573,496 - +0.5 2,573,496 - +0.3 2,573,496 - +0.4 2,573,497 - +0.2 2,573,550 + b2462 b2462 0.17 -0.3 2,573,560 - b2462 b2462 0.20 +2.0 2,573,562 - b2462 b2462 0.21 +0.7 2,573,562 - b2462 b2462 0.21 +1.3 2,573,562 - b2462 b2462 0.21 +0.7 2,573,572 - b2462 b2462 0.24 -1.0 2,573,572 - b2462 b2462 0.24 +0.0 2,573,581 - b2462 b2462 0.26 -0.3 2,573,581 - b2462 b2462 0.26 +0.0 2,573,604 - b2462 b2462 0.33 -1.1 2,573,606 + b2462 b2462 0.34 +0.4 2,573,751 - b2462 b2462 0.77 +0.5 2,573,774 - b2462 b2462 0.84 -0.2 2,573,774 - b2462 b2462 0.84 +0.8 2,573,775 + b2462 b2462 0.84 -1.0 2,573,783 - b2462 b2462 0.87 -0.5 2,573,784 - b2462 b2462 0.87 -0.5 2,573,792 - b2462 b2462 0.89 +1.3 2,573,818 + +0.3 2,573,830 + -0.7 2,573,830 + +1.3 2,573,838 - +0.7 2,573,838 - -2.0 2,573,886 + -2.0 2,573,928 - -1.9 2,573,933 - -0.5 2,573,963 + +1.3 2,573,963 + -0.7 2,574,083 - -1.4 2,574,103 - -2.4 2,574,133 - +0.2 2,574,145 + -2.7 2,574,148 - +0.2 2,574,152 - -3.4 2,574,158 + -1.8 2,574,158 + -0.4 2,574,170 + -4.6 2,574,173 - -2.1 2,574,187 - -2.0 2,574,187 - -2.6 2,574,190 - -1.7 2,574,202 - -3.0 2,574,216 + -1.3 2,574,225 + -1.4 2,574,233 - -2.4 2,574,378 - maeB b2463 0.11 -1.9 2,574,380 + maeB b2463 0.11 -1.7
Or see this region's nucleotide sequence