Experiment: Glycolic Acid (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt atoE and atoB are separated by 30 nucleotides atoB and yfaP are separated by 73 nucleotides yfaP and yfaQ are separated by 4 nucleotides
b2223: atoE - short chain fatty acid transporter (NCBI), at 2,322,778 to 2,324,100
atoE
b2224: atoB - acetyl-CoA acetyltransferase (NCBI), at 2,324,131 to 2,325,315
atoB
b2225: yfaP - hypothetical protein (NCBI), at 2,325,389 to 2,326,165
yfaP
b2226: yfaQ - hypothetical protein (NCBI), at 2,326,170 to 2,327,819
yfaQ
Position (kb)
2324
2325
2326 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 2323.139 kb on + strand, within atoE at 2323.194 kb on - strand, within atoE at 2323.260 kb on + strand, within atoE at 2323.321 kb on - strand, within atoE at 2323.321 kb on - strand, within atoE at 2323.403 kb on + strand, within atoE at 2323.417 kb on - strand, within atoE at 2323.417 kb on - strand, within atoE at 2323.426 kb on - strand, within atoE at 2323.475 kb on - strand, within atoE at 2323.601 kb on + strand, within atoE at 2323.601 kb on + strand, within atoE at 2323.608 kb on + strand, within atoE at 2323.873 kb on + strand, within atoE at 2324.037 kb on + strand at 2324.037 kb on + strand at 2324.037 kb on + strand at 2324.148 kb on + strand at 2324.307 kb on - strand, within atoB at 2324.469 kb on + strand, within atoB at 2324.539 kb on - strand, within atoB at 2324.547 kb on + strand, within atoB at 2324.547 kb on + strand, within atoB at 2324.549 kb on + strand, within atoB at 2324.585 kb on - strand, within atoB at 2324.735 kb on + strand, within atoB at 2324.886 kb on - strand, within atoB at 2325.002 kb on - strand, within atoB at 2325.006 kb on - strand, within atoB at 2325.077 kb on - strand, within atoB at 2325.094 kb on + strand, within atoB at 2325.215 kb on - strand at 2325.222 kb on - strand at 2325.224 kb on - strand at 2325.224 kb on - strand at 2325.226 kb on + strand at 2325.354 kb on + strand at 2325.354 kb on + strand at 2325.393 kb on - strand at 2325.393 kb on - strand at 2325.488 kb on + strand, within yfaP at 2325.526 kb on + strand, within yfaP at 2325.537 kb on - strand, within yfaP at 2325.555 kb on - strand, within yfaP at 2325.623 kb on - strand, within yfaP at 2325.623 kb on - strand, within yfaP at 2325.656 kb on - strand, within yfaP at 2325.756 kb on + strand, within yfaP at 2325.759 kb on - strand, within yfaP at 2325.970 kb on + strand, within yfaP at 2325.970 kb on + strand, within yfaP at 2326.112 kb on - strand at 2326.112 kb on - strand at 2326.209 kb on + strand at 2326.209 kb on + strand at 2326.237 kb on - strand at 2326.286 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Glycolic Acid (C) remove 2,323,139 + atoE b2223 0.27 -0.3 2,323,194 - atoE b2223 0.31 +0.2 2,323,260 + atoE b2223 0.36 +0.5 2,323,321 - atoE b2223 0.41 -0.3 2,323,321 - atoE b2223 0.41 +0.3 2,323,403 + atoE b2223 0.47 +0.2 2,323,417 - atoE b2223 0.48 -0.9 2,323,417 - atoE b2223 0.48 +0.5 2,323,426 - atoE b2223 0.49 -0.3 2,323,475 - atoE b2223 0.53 -0.2 2,323,601 + atoE b2223 0.62 +0.8 2,323,601 + atoE b2223 0.62 +1.2 2,323,608 + atoE b2223 0.63 -0.0 2,323,873 + atoE b2223 0.83 +0.2 2,324,037 + -1.1 2,324,037 + +1.1 2,324,037 + +0.1 2,324,148 + +0.5 2,324,307 - atoB b2224 0.15 -0.5 2,324,469 + atoB b2224 0.29 +0.8 2,324,539 - atoB b2224 0.34 +0.8 2,324,547 + atoB b2224 0.35 +0.4 2,324,547 + atoB b2224 0.35 +1.5 2,324,549 + atoB b2224 0.35 -1.3 2,324,585 - atoB b2224 0.38 +0.7 2,324,735 + atoB b2224 0.51 -0.1 2,324,886 - atoB b2224 0.64 +0.4 2,325,002 - atoB b2224 0.74 -0.1 2,325,006 - atoB b2224 0.74 +0.1 2,325,077 - atoB b2224 0.80 +0.2 2,325,094 + atoB b2224 0.81 -1.0 2,325,215 - +0.5 2,325,222 - +2.3 2,325,224 - +1.5 2,325,224 - +1.9 2,325,226 + +4.2 2,325,354 + +2.3 2,325,354 + +0.7 2,325,393 - +0.5 2,325,393 - +0.9 2,325,488 + yfaP b2225 0.13 +0.2 2,325,526 + yfaP b2225 0.18 -0.1 2,325,537 - yfaP b2225 0.19 +0.5 2,325,555 - yfaP b2225 0.21 +0.1 2,325,623 - yfaP b2225 0.30 +0.9 2,325,623 - yfaP b2225 0.30 +1.0 2,325,656 - yfaP b2225 0.34 +0.1 2,325,756 + yfaP b2225 0.47 +0.8 2,325,759 - yfaP b2225 0.48 +0.1 2,325,970 + yfaP b2225 0.75 +1.1 2,325,970 + yfaP b2225 0.75 +0.5 2,326,112 - +0.3 2,326,112 - +1.4 2,326,209 + -0.1 2,326,209 + +0.9 2,326,237 - +2.7 2,326,286 - +0.4
Or see this region's nucleotide sequence