Experiment: Glycolic Acid (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt adhP and sfcA are separated by 133 nucleotides sfcA and sra are separated by 156 nucleotides sra and b1481 are separated by 101 nucleotides b1481 and osmC are separated by 344 nucleotides
b1478: adhP - alcohol dehydrogenase (VIMSS), at 1,550,852 to 1,551,862
adhP
b1479: sfcA - NAD-linked malate dehydrogenase (malic enzyme) (VIMSS), at 1,551,996 to 1,553,693
sfcA
b1480: sra - 30S ribosomal subunit protein S22 (NCBI), at 1,553,850 to 1,553,987
sra
b1481: b1481 - orf, hypothetical protein (VIMSS), at 1,554,089 to 1,554,304
b1481
b1482: osmC - osmotically inducible, stress-inducible membrane protein (NCBI), at 1,554,649 to 1,555,080
osmC
Position (kb)
1551
1552
1553
1554 Strain fitness (log2 ratio)
-2
-1
0
1 at 1551.011 kb on + strand, within adhP at 1551.036 kb on - strand, within adhP at 1551.421 kb on + strand, within adhP at 1551.752 kb on + strand, within adhP at 1551.859 kb on + strand at 1551.859 kb on + strand at 1551.871 kb on - strand at 1552.077 kb on + strand at 1552.077 kb on + strand at 1552.093 kb on + strand at 1552.254 kb on - strand, within sfcA at 1552.274 kb on - strand, within sfcA at 1552.501 kb on + strand, within sfcA at 1552.509 kb on - strand, within sfcA at 1552.509 kb on - strand, within sfcA at 1552.638 kb on - strand, within sfcA at 1552.638 kb on - strand, within sfcA at 1553.155 kb on + strand, within sfcA at 1553.284 kb on - strand, within sfcA at 1553.427 kb on + strand, within sfcA at 1553.435 kb on - strand, within sfcA at 1553.588 kb on + strand at 1553.636 kb on + strand at 1553.636 kb on + strand at 1553.638 kb on + strand at 1553.647 kb on + strand at 1553.700 kb on - strand at 1553.833 kb on + strand at 1553.833 kb on + strand at 1553.951 kb on + strand, within sra at 1553.951 kb on + strand, within sra at 1553.951 kb on + strand, within sra at 1554.079 kb on + strand at 1554.082 kb on - strand at 1554.097 kb on - strand at 1554.101 kb on + strand at 1554.111 kb on - strand, within b1481 at 1554.111 kb on - strand, within b1481 at 1554.123 kb on - strand, within b1481 at 1554.132 kb on + strand, within b1481 at 1554.144 kb on - strand, within b1481 at 1554.218 kb on + strand, within b1481 at 1554.224 kb on - strand, within b1481 at 1554.224 kb on - strand, within b1481 at 1554.244 kb on - strand, within b1481 at 1554.249 kb on + strand, within b1481 at 1554.257 kb on - strand, within b1481 at 1554.273 kb on + strand, within b1481 at 1554.355 kb on - strand at 1554.365 kb on + strand at 1554.407 kb on + strand at 1554.407 kb on + strand at 1554.599 kb on + strand at 1554.599 kb on + strand at 1554.601 kb on + strand at 1554.639 kb on - strand at 1554.678 kb on + strand at 1554.678 kb on - strand at 1554.684 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Glycolic Acid (C) remove 1,551,011 + adhP b1478 0.16 -0.5 1,551,036 - adhP b1478 0.18 -0.3 1,551,421 + adhP b1478 0.56 -0.4 1,551,752 + adhP b1478 0.89 +0.7 1,551,859 + +0.0 1,551,859 + +0.0 1,551,871 - +1.5 1,552,077 + +0.4 1,552,077 + -0.4 1,552,093 + -1.2 1,552,254 - sfcA b1479 0.15 +0.1 1,552,274 - sfcA b1479 0.16 +0.8 1,552,501 + sfcA b1479 0.30 -0.1 1,552,509 - sfcA b1479 0.30 +0.0 1,552,509 - sfcA b1479 0.30 +0.4 1,552,638 - sfcA b1479 0.38 +0.2 1,552,638 - sfcA b1479 0.38 +0.6 1,553,155 + sfcA b1479 0.68 +0.2 1,553,284 - sfcA b1479 0.76 +1.0 1,553,427 + sfcA b1479 0.84 -0.2 1,553,435 - sfcA b1479 0.85 -0.7 1,553,588 + -1.5 1,553,636 + +0.1 1,553,636 + +0.3 1,553,638 + -0.5 1,553,647 + +0.1 1,553,700 - -0.2 1,553,833 + +0.3 1,553,833 + +1.0 1,553,951 + sra b1480 0.73 -0.9 1,553,951 + sra b1480 0.73 +0.4 1,553,951 + sra b1480 0.73 -1.2 1,554,079 + -0.3 1,554,082 - -0.8 1,554,097 - +0.0 1,554,101 + +0.4 1,554,111 - b1481 b1481 0.10 +0.5 1,554,111 - b1481 b1481 0.10 -0.2 1,554,123 - b1481 b1481 0.16 +0.1 1,554,132 + b1481 b1481 0.20 -0.5 1,554,144 - b1481 b1481 0.25 -0.8 1,554,218 + b1481 b1481 0.60 +0.2 1,554,224 - b1481 b1481 0.62 -0.6 1,554,224 - b1481 b1481 0.62 -0.5 1,554,244 - b1481 b1481 0.72 -0.8 1,554,249 + b1481 b1481 0.74 +0.3 1,554,257 - b1481 b1481 0.78 -0.2 1,554,273 + b1481 b1481 0.85 +0.6 1,554,355 - -1.8 1,554,365 + +0.8 1,554,407 + -1.7 1,554,407 + -1.8 1,554,599 + -0.9 1,554,599 + +1.1 1,554,601 + -0.5 1,554,639 - +0.1 1,554,678 + +1.0 1,554,678 - +0.4 1,554,684 - -0.2
Or see this region's nucleotide sequence