Strain Fitness in Escherichia coli BW25113 around b1186

Experiment: Glycolic Acid (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntumuC and dsbB are separated by 45 nucleotidesdsbB and nhaB are separated by 145 nucleotidesnhaB and fadR are separated by 220 nucleotidesfadR and ycgB are separated by 51 nucleotides b1184: umuC - DNA polymerase V subunit UmuC (NCBI), at 1,230,409 to 1,231,677 umuC b1185: dsbB - reoxidizes DsbA protein following formation of disulfide bond in P-ring of flagella. (VIMSS), at 1,231,723 to 1,232,253 dsbB b1186: nhaB - NhaB sodium/proton transporter (Katherine Huang), at 1,232,399 to 1,233,940 nhaB b1187: fadR - fatty acid metabolism regulator (NCBI), at 1,234,161 to 1,234,880 fadR b1188: ycgB - ycgB component of nitrogen-related signalling system (of yeaGH-ycgB) (from data), at 1,234,932 to 1,236,464 ycgB Position (kb) 1232 1233 1234Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4at 1231.457 kb on + strand, within umuCat 1231.519 kb on - strand, within umuCat 1231.550 kb on + strand, within umuCat 1231.551 kb on - strandat 1231.553 kb on - strandat 1231.573 kb on - strandat 1231.597 kb on + strandat 1231.625 kb on + strandat 1231.627 kb on - strandat 1231.643 kb on - strandat 1231.645 kb on - strandat 1231.660 kb on - strandat 1231.961 kb on + strand, within dsbBat 1231.961 kb on + strand, within dsbBat 1231.979 kb on - strand, within dsbBat 1232.074 kb on + strand, within dsbBat 1232.074 kb on + strand, within dsbBat 1232.077 kb on - strand, within dsbBat 1232.272 kb on + strandat 1232.272 kb on + strandat 1232.504 kb on - strandat 1232.509 kb on - strandat 1232.525 kb on + strandat 1232.569 kb on - strand, within nhaBat 1232.784 kb on - strand, within nhaBat 1232.784 kb on - strand, within nhaBat 1232.921 kb on - strand, within nhaBat 1232.921 kb on - strand, within nhaBat 1232.936 kb on + strand, within nhaBat 1233.026 kb on + strand, within nhaBat 1233.087 kb on - strand, within nhaBat 1233.196 kb on - strand, within nhaBat 1233.269 kb on + strand, within nhaBat 1233.277 kb on - strand, within nhaBat 1233.310 kb on - strand, within nhaBat 1233.518 kb on + strand, within nhaBat 1233.518 kb on + strand, within nhaBat 1233.521 kb on - strand, within nhaBat 1233.652 kb on - strand, within nhaBat 1233.686 kb on + strand, within nhaBat 1233.723 kb on - strand, within nhaBat 1233.736 kb on - strand, within nhaBat 1233.736 kb on - strand, within nhaBat 1233.764 kb on - strand, within nhaBat 1233.764 kb on - strand, within nhaBat 1233.923 kb on + strandat 1233.923 kb on + strandat 1233.931 kb on - strandat 1234.004 kb on - strandat 1234.099 kb on - strandat 1234.102 kb on + strandat 1234.112 kb on + strandat 1234.114 kb on + strandat 1234.118 kb on - strandat 1234.118 kb on - strandat 1234.157 kb on + strandat 1234.222 kb on + strandat 1234.299 kb on + strand, within fadRat 1234.317 kb on - strand, within fadRat 1234.331 kb on + strand, within fadRat 1234.436 kb on - strandat 1234.565 kb on + strand, within fadRat 1234.673 kb on + strand, within fadRat 1234.681 kb on - strand, within fadRat 1234.720 kb on + strand, within fadRat 1234.807 kb on + strand, within fadRat 1234.807 kb on + strand, within fadRat 1234.815 kb on - strandat 1234.889 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Glycolic Acid (C)
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1,231,457 + umuC b1184 0.83 -0.5
1,231,519 - umuC b1184 0.87 +0.1
1,231,550 + umuC b1184 0.90 -1.6
1,231,551 - -0.2
1,231,553 - +0.7
1,231,573 - -0.3
1,231,597 + +0.0
1,231,625 + -0.3
1,231,627 - +1.3
1,231,643 - -0.6
1,231,645 - +0.5
1,231,660 - +0.4
1,231,961 + dsbB b1185 0.45 +0.7
1,231,961 + dsbB b1185 0.45 +0.7
1,231,979 - dsbB b1185 0.48 -1.4
1,232,074 + dsbB b1185 0.66 -0.9
1,232,074 + dsbB b1185 0.66 -0.4
1,232,077 - dsbB b1185 0.67 +1.4
1,232,272 + +0.7
1,232,272 + -0.4
1,232,504 - +1.0
1,232,509 - +0.4
1,232,525 + +0.7
1,232,569 - nhaB b1186 0.11 +0.4
1,232,784 - nhaB b1186 0.25 +4.0
1,232,784 - nhaB b1186 0.25 +1.0
1,232,921 - nhaB b1186 0.34 +1.1
1,232,921 - nhaB b1186 0.34 +0.4
1,232,936 + nhaB b1186 0.35 +0.7
1,233,026 + nhaB b1186 0.41 +0.7
1,233,087 - nhaB b1186 0.45 +1.0
1,233,196 - nhaB b1186 0.52 +0.4
1,233,269 + nhaB b1186 0.56 +1.4
1,233,277 - nhaB b1186 0.57 +0.9
1,233,310 - nhaB b1186 0.59 +1.1
1,233,518 + nhaB b1186 0.73 -0.7
1,233,518 + nhaB b1186 0.73 -1.7
1,233,521 - nhaB b1186 0.73 +0.0
1,233,652 - nhaB b1186 0.81 -0.0
1,233,686 + nhaB b1186 0.83 -0.2
1,233,723 - nhaB b1186 0.86 +0.8
1,233,736 - nhaB b1186 0.87 -1.5
1,233,736 - nhaB b1186 0.87 -0.8
1,233,764 - nhaB b1186 0.89 +0.3
1,233,764 - nhaB b1186 0.89 +0.5
1,233,923 + +1.0
1,233,923 + +0.2
1,233,931 - +0.5
1,234,004 - -0.9
1,234,099 - -0.4
1,234,102 + +1.2
1,234,112 + -0.6
1,234,114 + -1.6
1,234,118 - -0.4
1,234,118 - -1.4
1,234,157 + +0.9
1,234,222 + -2.6
1,234,299 + fadR b1187 0.19 -3.3
1,234,317 - fadR b1187 0.22 -1.9
1,234,331 + fadR b1187 0.24 -1.9
1,234,436 - +0.4
1,234,565 + fadR b1187 0.56 -2.4
1,234,673 + fadR b1187 0.71 -1.1
1,234,681 - fadR b1187 0.72 -1.4
1,234,720 + fadR b1187 0.78 -0.6
1,234,807 + fadR b1187 0.90 -1.8
1,234,807 + fadR b1187 0.90 -2.2
1,234,815 - -2.2
1,234,889 - +0.5

Or see this region's nucleotide sequence