Experiment: Glycolic Acid (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt aspC and ompF are separated by 184 nucleotides ompF and asnS are separated by 602 nucleotides
b0928: aspC - aspartate aminotransferase, PLP-dependent (NCBI), at 983,742 to 984,932
aspC
b0929: ompF - outer membrane porin 1a (Ia;b;F) (NCBI), at 985,117 to 986,205
ompF
b0930: asnS - asparaginyl-tRNA synthetase (NCBI), at 986,808 to 988,208
asnS
Position (kb) 985
986
987 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 984.232 kb on - strand, within aspC at 984.326 kb on + strand, within aspC at 984.365 kb on - strand, within aspC at 984.402 kb on + strand, within aspC at 984.402 kb on + strand, within aspC at 984.535 kb on - strand, within aspC at 984.560 kb on + strand, within aspC at 984.560 kb on + strand, within aspC at 984.633 kb on + strand, within aspC at 984.633 kb on + strand, within aspC at 984.683 kb on - strand, within aspC at 984.792 kb on + strand, within aspC at 984.939 kb on + strand at 984.942 kb on + strand at 984.944 kb on + strand at 984.975 kb on + strand at 985.123 kb on - strand at 985.169 kb on + strand at 985.240 kb on + strand, within ompF at 985.259 kb on - strand, within ompF at 985.483 kb on + strand, within ompF at 985.548 kb on - strand, within ompF at 985.548 kb on - strand, within ompF at 985.682 kb on + strand, within ompF at 985.682 kb on + strand, within ompF at 985.805 kb on + strand, within ompF at 985.813 kb on - strand, within ompF at 985.880 kb on - strand, within ompF at 985.934 kb on + strand, within ompF at 985.934 kb on + strand, within ompF at 986.017 kb on + strand, within ompF at 986.017 kb on + strand, within ompF at 986.164 kb on - strand at 986.164 kb on - strand at 986.179 kb on - strand at 986.217 kb on - strand at 986.217 kb on - strand at 986.445 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Glycolic Acid (C) remove 984,232 - aspC b0928 0.41 -0.8 984,326 + aspC b0928 0.49 -1.9 984,365 - aspC b0928 0.52 -3.5 984,402 + aspC b0928 0.55 -2.0 984,402 + aspC b0928 0.55 -3.2 984,535 - aspC b0928 0.67 -4.0 984,560 + aspC b0928 0.69 -0.6 984,560 + aspC b0928 0.69 -0.2 984,633 + aspC b0928 0.75 -1.2 984,633 + aspC b0928 0.75 -0.2 984,683 - aspC b0928 0.79 -1.8 984,792 + aspC b0928 0.88 -2.2 984,939 + -1.0 984,942 + -1.7 984,944 + -1.3 984,975 + -1.3 985,123 - -1.0 985,169 + +0.1 985,240 + ompF b0929 0.11 -1.4 985,259 - ompF b0929 0.13 -0.5 985,483 + ompF b0929 0.34 -2.0 985,548 - ompF b0929 0.40 -1.3 985,548 - ompF b0929 0.40 -2.1 985,682 + ompF b0929 0.52 -1.4 985,682 + ompF b0929 0.52 -0.7 985,805 + ompF b0929 0.63 -0.7 985,813 - ompF b0929 0.64 -2.5 985,880 - ompF b0929 0.70 -1.9 985,934 + ompF b0929 0.75 -0.9 985,934 + ompF b0929 0.75 -0.7 986,017 + ompF b0929 0.83 -0.1 986,017 + ompF b0929 0.83 +0.9 986,164 - +0.2 986,164 - -0.9 986,179 - -1.5 986,217 - -1.9 986,217 - -1.2 986,445 - -0.8
Or see this region's nucleotide sequence