Experiment: Glycolic Acid (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ybeZ and miaB are separated by 152 nucleotides miaB and ubiF are separated by 145 nucleotides
b0660: ybeZ - predicted protein with nucleoside triphosphate hydrolase domain (RefSeq), at 691,561 to 692,601
ybeZ
b0661: miaB - isopentenyl-adenosine A37 tRNA methylthiolase (NCBI), at 692,754 to 694,178
miaB
b0662: ubiF - 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase (NCBI), at 694,324 to 695,499
ubiF
Position (kb) 692
693
694
695 Strain fitness (log2 ratio) -2
-1
0
1
2 at 691.757 kb on + strand, within ybeZ at 691.757 kb on + strand, within ybeZ at 691.766 kb on - strand, within ybeZ at 691.793 kb on - strand, within ybeZ at 691.793 kb on - strand, within ybeZ at 691.842 kb on + strand, within ybeZ at 691.849 kb on - strand, within ybeZ at 692.300 kb on - strand, within ybeZ at 692.300 kb on - strand, within ybeZ at 692.407 kb on - strand, within ybeZ at 692.407 kb on - strand, within ybeZ at 692.576 kb on - strand at 692.576 kb on - strand at 692.598 kb on - strand at 692.598 kb on - strand at 692.774 kb on + strand at 692.914 kb on + strand, within miaB at 693.086 kb on + strand, within miaB at 693.088 kb on + strand, within miaB at 693.088 kb on + strand, within miaB at 693.141 kb on + strand, within miaB at 693.175 kb on + strand, within miaB at 693.175 kb on - strand, within miaB at 693.175 kb on - strand, within miaB at 693.183 kb on - strand, within miaB at 693.183 kb on - strand, within miaB at 693.236 kb on - strand, within miaB at 693.302 kb on + strand, within miaB at 693.310 kb on - strand, within miaB at 693.310 kb on - strand, within miaB at 693.310 kb on - strand, within miaB at 693.310 kb on - strand, within miaB at 693.337 kb on - strand, within miaB at 693.380 kb on + strand, within miaB at 693.380 kb on + strand, within miaB at 693.380 kb on + strand, within miaB at 693.595 kb on - strand, within miaB at 693.662 kb on - strand, within miaB at 693.662 kb on - strand, within miaB at 693.662 kb on - strand, within miaB at 693.662 kb on - strand, within miaB at 693.682 kb on + strand, within miaB at 693.764 kb on - strand, within miaB at 693.798 kb on + strand, within miaB at 693.798 kb on + strand, within miaB at 693.813 kb on - strand, within miaB at 694.062 kb on - strand at 694.094 kb on - strand at 694.094 kb on - strand at 694.196 kb on + strand at 694.266 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Glycolic Acid (C) remove 691,757 + ybeZ b0660 0.19 +1.6 691,757 + ybeZ b0660 0.19 -0.1 691,766 - ybeZ b0660 0.20 -1.6 691,793 - ybeZ b0660 0.22 -0.3 691,793 - ybeZ b0660 0.22 -1.9 691,842 + ybeZ b0660 0.27 -1.2 691,849 - ybeZ b0660 0.28 -2.2 692,300 - ybeZ b0660 0.71 +1.1 692,300 - ybeZ b0660 0.71 +0.6 692,407 - ybeZ b0660 0.81 -0.6 692,407 - ybeZ b0660 0.81 -1.6 692,576 - +0.9 692,576 - +0.8 692,598 - +1.0 692,598 - +0.6 692,774 + -0.6 692,914 + miaB b0661 0.11 -0.3 693,086 + miaB b0661 0.23 -0.2 693,088 + miaB b0661 0.23 -1.0 693,088 + miaB b0661 0.23 +0.6 693,141 + miaB b0661 0.27 +0.2 693,175 + miaB b0661 0.30 +0.1 693,175 - miaB b0661 0.30 -1.8 693,175 - miaB b0661 0.30 +0.5 693,183 - miaB b0661 0.30 +0.8 693,183 - miaB b0661 0.30 -2.0 693,236 - miaB b0661 0.34 +0.2 693,302 + miaB b0661 0.38 -0.8 693,310 - miaB b0661 0.39 -0.7 693,310 - miaB b0661 0.39 -1.3 693,310 - miaB b0661 0.39 -1.7 693,310 - miaB b0661 0.39 +0.2 693,337 - miaB b0661 0.41 -0.9 693,380 + miaB b0661 0.44 -2.2 693,380 + miaB b0661 0.44 -1.5 693,380 + miaB b0661 0.44 +0.1 693,595 - miaB b0661 0.59 +0.5 693,662 - miaB b0661 0.64 +0.4 693,662 - miaB b0661 0.64 +0.8 693,662 - miaB b0661 0.64 +0.3 693,662 - miaB b0661 0.64 +0.8 693,682 + miaB b0661 0.65 -0.6 693,764 - miaB b0661 0.71 -0.7 693,798 + miaB b0661 0.73 -0.9 693,798 + miaB b0661 0.73 -0.5 693,813 - miaB b0661 0.74 +0.1 694,062 - +0.1 694,094 - +0.3 694,094 - +2.0 694,196 + +0.7 694,266 + -1.2
Or see this region's nucleotide sequence