Experiment: Glycolic Acid (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt metQ and metI are separated by 39 nucleotides metI and metN overlap by 8 nucleotides metN and gmhB are separated by 187 nucleotides
b0197: metQ - DL-methionine transporter subunit (NCBI), at 220,113 to 220,928
metQ
b0198: metI - DL-methionine transporter subunit (NCBI), at 220,968 to 221,621
metI
b0199: metN - DL-methionine transporter subunit (NCBI), at 221,614 to 222,645
metN
b0200: gmhB - hypothetical protein (NCBI), at 222,833 to 223,408
gmhB
Position (kb) 221
222
223 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 220.661 kb on + strand, within metQ at 220.675 kb on + strand, within metQ at 220.694 kb on - strand, within metQ at 220.694 kb on - strand, within metQ at 220.750 kb on - strand, within metQ at 220.754 kb on + strand, within metQ at 220.764 kb on + strand, within metQ at 220.825 kb on + strand, within metQ at 220.865 kb on + strand at 221.043 kb on - strand, within metI at 221.047 kb on + strand, within metI at 221.052 kb on + strand, within metI at 221.060 kb on - strand, within metI at 221.076 kb on + strand, within metI at 221.076 kb on + strand, within metI at 221.080 kb on + strand, within metI at 221.080 kb on + strand, within metI at 221.096 kb on - strand, within metI at 221.105 kb on - strand, within metI at 221.106 kb on - strand, within metI at 221.128 kb on - strand, within metI at 221.148 kb on + strand, within metI at 221.148 kb on + strand, within metI at 221.181 kb on + strand, within metI at 221.214 kb on + strand, within metI at 221.238 kb on + strand, within metI at 221.238 kb on + strand, within metI at 221.250 kb on - strand, within metI at 221.269 kb on - strand, within metI at 221.280 kb on + strand, within metI at 221.300 kb on - strand, within metI at 221.347 kb on - strand, within metI at 221.383 kb on - strand, within metI at 221.383 kb on - strand, within metI at 221.466 kb on - strand, within metI at 221.469 kb on - strand, within metI at 221.511 kb on - strand, within metI at 221.511 kb on - strand, within metI at 221.595 kb on - strand at 221.595 kb on - strand at 221.619 kb on + strand at 221.623 kb on + strand at 221.655 kb on - strand at 221.680 kb on + strand at 221.685 kb on + strand at 221.688 kb on - strand at 221.802 kb on + strand, within metN at 221.830 kb on + strand, within metN at 221.830 kb on + strand, within metN at 221.846 kb on + strand, within metN at 221.847 kb on + strand, within metN at 221.848 kb on + strand, within metN at 221.856 kb on + strand, within metN at 221.911 kb on - strand, within metN at 221.967 kb on + strand, within metN at 221.967 kb on + strand, within metN at 221.975 kb on - strand, within metN at 221.999 kb on - strand, within metN at 222.001 kb on + strand, within metN at 222.009 kb on - strand, within metN at 222.027 kb on - strand, within metN at 222.061 kb on - strand, within metN at 222.102 kb on - strand, within metN at 222.102 kb on - strand, within metN at 222.159 kb on + strand, within metN at 222.203 kb on + strand, within metN at 222.239 kb on + strand, within metN at 222.239 kb on + strand, within metN at 222.247 kb on + strand, within metN at 222.269 kb on - strand, within metN at 222.279 kb on - strand, within metN at 222.364 kb on - strand, within metN at 222.425 kb on - strand, within metN at 222.495 kb on - strand, within metN at 222.501 kb on + strand, within metN at 222.501 kb on + strand, within metN at 222.507 kb on - strand, within metN at 222.511 kb on + strand, within metN at 222.520 kb on - strand, within metN at 222.578 kb on - strand at 222.588 kb on - strand at 222.588 kb on - strand at 222.594 kb on - strand at 222.594 kb on - strand at 222.620 kb on - strand at 222.731 kb on - strand at 222.732 kb on + strand at 222.732 kb on + strand at 222.813 kb on + strand at 222.907 kb on - strand, within gmhB at 222.907 kb on - strand, within gmhB at 222.941 kb on - strand, within gmhB at 223.224 kb on + strand, within gmhB at 223.232 kb on - strand, within gmhB at 223.232 kb on - strand, within gmhB at 223.477 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Glycolic Acid (C) remove 220,661 + metQ b0197 0.67 -1.1 220,675 + metQ b0197 0.69 -0.3 220,694 - metQ b0197 0.71 -0.5 220,694 - metQ b0197 0.71 -3.0 220,750 - metQ b0197 0.78 -0.9 220,754 + metQ b0197 0.79 -1.0 220,764 + metQ b0197 0.80 -0.0 220,825 + metQ b0197 0.87 -0.1 220,865 + -0.4 221,043 - metI b0198 0.11 +0.2 221,047 + metI b0198 0.12 -0.4 221,052 + metI b0198 0.13 -2.4 221,060 - metI b0198 0.14 +0.0 221,076 + metI b0198 0.17 -1.4 221,076 + metI b0198 0.17 -1.3 221,080 + metI b0198 0.17 -2.6 221,080 + metI b0198 0.17 -0.6 221,096 - metI b0198 0.20 -1.4 221,105 - metI b0198 0.21 -0.6 221,106 - metI b0198 0.21 -1.4 221,128 - metI b0198 0.24 -1.4 221,148 + metI b0198 0.28 +0.2 221,148 + metI b0198 0.28 -0.9 221,181 + metI b0198 0.33 -1.9 221,214 + metI b0198 0.38 -0.2 221,238 + metI b0198 0.41 -0.8 221,238 + metI b0198 0.41 -1.7 221,250 - metI b0198 0.43 -1.7 221,269 - metI b0198 0.46 -0.9 221,280 + metI b0198 0.48 -2.7 221,300 - metI b0198 0.51 -1.2 221,347 - metI b0198 0.58 -2.0 221,383 - metI b0198 0.63 -1.0 221,383 - metI b0198 0.63 -1.0 221,466 - metI b0198 0.76 -1.3 221,469 - metI b0198 0.77 -1.0 221,511 - metI b0198 0.83 -0.5 221,511 - metI b0198 0.83 +0.1 221,595 - +0.2 221,595 - -1.0 221,619 + -1.3 221,623 + -0.7 221,655 - -0.4 221,680 + -1.7 221,685 + -1.4 221,688 - -0.5 221,802 + metN b0199 0.18 -1.0 221,830 + metN b0199 0.21 -0.6 221,830 + metN b0199 0.21 -0.0 221,846 + metN b0199 0.22 -1.8 221,847 + metN b0199 0.23 -1.5 221,848 + metN b0199 0.23 -0.6 221,856 + metN b0199 0.23 -0.7 221,911 - metN b0199 0.29 +0.8 221,967 + metN b0199 0.34 -0.7 221,967 + metN b0199 0.34 -0.5 221,975 - metN b0199 0.35 -0.2 221,999 - metN b0199 0.37 +0.2 222,001 + metN b0199 0.38 -2.2 222,009 - metN b0199 0.38 -1.0 222,027 - metN b0199 0.40 -1.1 222,061 - metN b0199 0.43 +0.5 222,102 - metN b0199 0.47 -0.2 222,102 - metN b0199 0.47 -0.0 222,159 + metN b0199 0.53 -0.5 222,203 + metN b0199 0.57 -1.1 222,239 + metN b0199 0.61 -1.6 222,239 + metN b0199 0.61 -0.4 222,247 + metN b0199 0.61 -0.4 222,269 - metN b0199 0.63 -0.4 222,279 - metN b0199 0.64 -1.8 222,364 - metN b0199 0.73 -1.0 222,425 - metN b0199 0.79 +0.0 222,495 - metN b0199 0.85 -2.2 222,501 + metN b0199 0.86 -1.4 222,501 + metN b0199 0.86 -0.3 222,507 - metN b0199 0.87 -0.5 222,511 + metN b0199 0.87 -2.0 222,520 - metN b0199 0.88 -1.4 222,578 - -1.0 222,588 - -0.0 222,588 - -0.9 222,594 - +0.7 222,594 - +0.0 222,620 - -0.8 222,731 - -0.8 222,732 + -1.2 222,732 + +0.9 222,813 + -0.6 222,907 - gmhB b0200 0.13 -0.6 222,907 - gmhB b0200 0.13 -0.3 222,941 - gmhB b0200 0.19 -2.0 223,224 + gmhB b0200 0.68 -0.8 223,232 - gmhB b0200 0.69 +1.7 223,232 - gmhB b0200 0.69 -0.6 223,477 - -0.3
Or see this region's nucleotide sequence