Strain Fitness in Escherichia coli BW25113 around b0127

Experiment: Glycolic Acid (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthpt and can are separated by 40 nucleotidescan and yadG are separated by 108 nucleotidesyadG and yadH overlap by 4 nucleotidesyadH and yadI are separated by 104 nucleotides b0125: hpt - hypoxanthine phosphoribosyltransferase (VIMSS), at 141,431 to 141,967 hpt b0126: can - carbonic anhydrase (NCBI), at 142,008 to 142,670 can b0127: yadG - predicted transporter subunit: ATP-binding component of ABC superfamily (NCBI), at 142,779 to 143,705 yadG b0128: yadH - predicted transporter subunit: membrane component of ABC superfamily (NCBI), at 143,702 to 144,472 yadH b0129: yadI - predicted PTS Enzyme IIA (NCBI), at 144,577 to 145,017 yadI Position (kb) 142 143 144Strain fitness (log2 ratio) -3 -2 -1 0 1at 141.899 kb on + strand, within hptat 141.906 kb on + strand, within hptat 141.910 kb on + strand, within hptat 141.910 kb on + strand, within hptat 141.967 kb on + strandat 142.000 kb on - strandat 142.000 kb on - strandat 142.184 kb on - strand, within canat 142.836 kb on - strandat 142.836 kb on - strandat 142.925 kb on - strand, within yadGat 143.022 kb on - strand, within yadGat 143.051 kb on + strand, within yadGat 143.490 kb on - strand, within yadGat 143.532 kb on - strand, within yadGat 143.532 kb on - strand, within yadGat 143.532 kb on - strand, within yadGat 143.554 kb on + strand, within yadGat 143.554 kb on + strand, within yadGat 143.585 kb on - strand, within yadGat 143.590 kb on + strand, within yadGat 143.590 kb on + strand, within yadGat 143.607 kb on - strand, within yadGat 143.620 kb on - strandat 143.621 kb on + strandat 143.744 kb on + strandat 143.757 kb on + strandat 143.782 kb on - strand, within yadHat 143.790 kb on + strand, within yadHat 143.815 kb on + strand, within yadHat 143.824 kb on + strand, within yadHat 143.845 kb on + strand, within yadHat 143.879 kb on + strand, within yadHat 143.899 kb on + strand, within yadHat 143.912 kb on + strand, within yadHat 143.986 kb on + strand, within yadHat 144.160 kb on - strand, within yadHat 144.218 kb on + strand, within yadHat 144.256 kb on - strand, within yadHat 144.302 kb on + strand, within yadHat 144.304 kb on + strand, within yadHat 144.304 kb on + strand, within yadHat 144.331 kb on + strand, within yadHat 144.333 kb on + strand, within yadHat 144.386 kb on + strand, within yadHat 144.386 kb on + strand, within yadHat 144.403 kb on + strandat 144.403 kb on + strandat 144.440 kb on - strandat 144.539 kb on + strandat 144.539 kb on + strandat 144.539 kb on + strandat 144.539 kb on + strandat 144.630 kb on - strand, within yadIat 144.630 kb on - strand, within yadIat 144.663 kb on + strand, within yadIat 144.663 kb on + strand, within yadI

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Per-strain Table

Position Strand Gene LocusTag Fraction Glycolic Acid (C)
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141,899 + hpt b0125 0.87 +1.7
141,906 + hpt b0125 0.88 +0.9
141,910 + hpt b0125 0.89 +1.7
141,910 + hpt b0125 0.89 +1.0
141,967 + -1.3
142,000 - -0.3
142,000 - +1.4
142,184 - can b0126 0.27 -0.8
142,836 - -0.2
142,836 - -0.4
142,925 - yadG b0127 0.16 -1.2
143,022 - yadG b0127 0.26 -0.6
143,051 + yadG b0127 0.29 +0.1
143,490 - yadG b0127 0.77 -0.5
143,532 - yadG b0127 0.81 -1.0
143,532 - yadG b0127 0.81 -1.6
143,532 - yadG b0127 0.81 -2.6
143,554 + yadG b0127 0.84 -3.2
143,554 + yadG b0127 0.84 -1.2
143,585 - yadG b0127 0.87 -0.3
143,590 + yadG b0127 0.87 -1.3
143,590 + yadG b0127 0.87 -0.3
143,607 - yadG b0127 0.89 +0.6
143,620 - +0.6
143,621 + -1.5
143,744 + -0.0
143,757 + -0.1
143,782 - yadH b0128 0.10 -0.7
143,790 + yadH b0128 0.11 -0.4
143,815 + yadH b0128 0.15 -1.0
143,824 + yadH b0128 0.16 -1.6
143,845 + yadH b0128 0.19 +0.8
143,879 + yadH b0128 0.23 -0.2
143,899 + yadH b0128 0.26 -1.5
143,912 + yadH b0128 0.27 -1.1
143,986 + yadH b0128 0.37 -0.8
144,160 - yadH b0128 0.59 -0.6
144,218 + yadH b0128 0.67 -1.7
144,256 - yadH b0128 0.72 -1.2
144,302 + yadH b0128 0.78 -1.6
144,304 + yadH b0128 0.78 -0.3
144,304 + yadH b0128 0.78 +0.0
144,331 + yadH b0128 0.82 +0.0
144,333 + yadH b0128 0.82 -0.3
144,386 + yadH b0128 0.89 +0.7
144,386 + yadH b0128 0.89 -1.8
144,403 + +0.5
144,403 + -0.4
144,440 - -0.7
144,539 + +0.5
144,539 + +0.7
144,539 + -0.1
144,539 + -0.1
144,630 - yadI b0129 0.12 -0.3
144,630 - yadI b0129 0.12 -0.1
144,663 + yadI b0129 0.20 +0.6
144,663 + yadI b0129 0.20 +0.6

Or see this region's nucleotide sequence