Experiment: MS_media with arsenate 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3542 and EX28DRAFT_3543 are separated by 9 nucleotides EX28DRAFT_3543 and EX28DRAFT_3544 are separated by 65 nucleotides EX28DRAFT_3544 and EX28DRAFT_3545 are separated by 9 nucleotides EX28DRAFT_3545 and EX28DRAFT_3546 are separated by 2 nucleotides
EX28DRAFT_3542: EX28DRAFT_3542 - 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase, at 206,612 to 207,409
_3542
EX28DRAFT_3543: EX28DRAFT_3543 - 2-oxo-hepta-3-ene-1,7-dioic acid hydratase, at 207,419 to 208,222
_3543
EX28DRAFT_3544: EX28DRAFT_3544 - 5-carboxymethyl-2-hydroxymuconate isomerase, at 208,288 to 208,668
_3544
EX28DRAFT_3545: EX28DRAFT_3545 - 3,4-dihydroxyphenylacetate 2,3-dioxygenase, at 208,678 to 209,529
_3545
EX28DRAFT_3546: EX28DRAFT_3546 - 5-carboxymethyl-2-hydroxymuconate semialdehyde dehydrogenase, at 209,532 to 210,998
_3546
Position (kb) 208
209 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 207.333 kb on + strand at 207.333 kb on + strand at 207.334 kb on - strand at 207.334 kb on - strand at 207.334 kb on - strand at 207.543 kb on - strand, within EX28DRAFT_3543 at 207.598 kb on + strand, within EX28DRAFT_3543 at 207.689 kb on - strand, within EX28DRAFT_3543 at 207.825 kb on + strand, within EX28DRAFT_3543 at 207.826 kb on - strand, within EX28DRAFT_3543 at 207.826 kb on - strand, within EX28DRAFT_3543 at 207.984 kb on + strand, within EX28DRAFT_3543 at 208.238 kb on + strand at 208.293 kb on + strand at 208.293 kb on + strand at 208.322 kb on + strand at 208.323 kb on - strand at 208.370 kb on + strand, within EX28DRAFT_3544 at 208.371 kb on - strand, within EX28DRAFT_3544 at 208.479 kb on + strand, within EX28DRAFT_3544 at 208.480 kb on - strand, within EX28DRAFT_3544 at 208.645 kb on - strand at 208.657 kb on - strand at 208.705 kb on - strand at 208.764 kb on - strand, within EX28DRAFT_3545 at 208.829 kb on + strand, within EX28DRAFT_3545 at 208.829 kb on + strand, within EX28DRAFT_3545 at 208.932 kb on - strand, within EX28DRAFT_3545 at 209.018 kb on + strand, within EX28DRAFT_3545 at 209.105 kb on - strand, within EX28DRAFT_3545 at 209.132 kb on + strand, within EX28DRAFT_3545 at 209.157 kb on - strand, within EX28DRAFT_3545 at 209.157 kb on - strand, within EX28DRAFT_3545 at 209.255 kb on + strand, within EX28DRAFT_3545 at 209.261 kb on + strand, within EX28DRAFT_3545 at 209.304 kb on - strand, within EX28DRAFT_3545 at 209.523 kb on + strand at 209.524 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with arsenate 5 mM remove 207,333 + -0.4 207,333 + +0.2 207,334 - +0.2 207,334 - +0.6 207,334 - +0.8 207,543 - EX28DRAFT_3543 0.15 -0.0 207,598 + EX28DRAFT_3543 0.22 -0.8 207,689 - EX28DRAFT_3543 0.34 -0.6 207,825 + EX28DRAFT_3543 0.50 +0.0 207,826 - EX28DRAFT_3543 0.51 -0.1 207,826 - EX28DRAFT_3543 0.51 -0.6 207,984 + EX28DRAFT_3543 0.70 -0.3 208,238 + +0.0 208,293 + +0.1 208,293 + -0.6 208,322 + +0.0 208,323 - -0.0 208,370 + EX28DRAFT_3544 0.22 +1.1 208,371 - EX28DRAFT_3544 0.22 -0.2 208,479 + EX28DRAFT_3544 0.50 +0.9 208,480 - EX28DRAFT_3544 0.50 +1.1 208,645 - -0.2 208,657 - -0.0 208,705 - -0.1 208,764 - EX28DRAFT_3545 0.10 +0.6 208,829 + EX28DRAFT_3545 0.18 -0.2 208,829 + EX28DRAFT_3545 0.18 +0.3 208,932 - EX28DRAFT_3545 0.30 +1.6 209,018 + EX28DRAFT_3545 0.40 +0.3 209,105 - EX28DRAFT_3545 0.50 -0.1 209,132 + EX28DRAFT_3545 0.53 +0.0 209,157 - EX28DRAFT_3545 0.56 -0.0 209,157 - EX28DRAFT_3545 0.56 -0.1 209,255 + EX28DRAFT_3545 0.68 -2.3 209,261 + EX28DRAFT_3545 0.68 -0.2 209,304 - EX28DRAFT_3545 0.73 -0.1 209,523 + -3.3 209,524 - +0.1
Or see this region's nucleotide sequence