Experiment: MS_media with arsenate 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2509 and EX28DRAFT_2510 are separated by 77 nucleotides EX28DRAFT_2510 and EX28DRAFT_2511 are separated by 86 nucleotides EX28DRAFT_2511 and EX28DRAFT_2512 are separated by 81 nucleotides EX28DRAFT_2512 and EX28DRAFT_2513 overlap by 4 nucleotides EX28DRAFT_2513 and EX28DRAFT_2514 are separated by 261 nucleotides
EX28DRAFT_2509: EX28DRAFT_2509 - transcriptional regulator, LacI family, at 2,541,449 to 2,542,519
_2509
EX28DRAFT_2510: EX28DRAFT_2510 - Predicted membrane protein, at 2,542,597 to 2,543,208
_2510
EX28DRAFT_2511: EX28DRAFT_2511 - Transcriptional regulators containing a DNA-binding HTH domain and an aminotransferase domain (MocR family) and their eukaryotic orthologs, at 2,543,295 to 2,544,716
_2511
EX28DRAFT_2512: EX28DRAFT_2512 - hypothetical protein, at 2,544,798 to 2,545,013
_2512
EX28DRAFT_2513: EX28DRAFT_2513 - hypothetical protein, at 2,545,010 to 2,545,261
_2513
EX28DRAFT_2514: EX28DRAFT_2514 - RNA, at 2,545,523 to 2,545,619
_2514
Position (kb)
2543
2544
2545 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2542.381 kb on - strand, within EX28DRAFT_2509 at 2542.500 kb on + strand at 2542.501 kb on - strand at 2542.501 kb on - strand at 2542.550 kb on + strand at 2542.672 kb on - strand, within EX28DRAFT_2510 at 2542.672 kb on - strand, within EX28DRAFT_2510 at 2542.694 kb on + strand, within EX28DRAFT_2510 at 2542.694 kb on + strand, within EX28DRAFT_2510 at 2542.695 kb on - strand, within EX28DRAFT_2510 at 2542.695 kb on - strand, within EX28DRAFT_2510 at 2542.695 kb on - strand, within EX28DRAFT_2510 at 2542.856 kb on + strand, within EX28DRAFT_2510 at 2542.856 kb on + strand, within EX28DRAFT_2510 at 2542.857 kb on - strand, within EX28DRAFT_2510 at 2542.857 kb on - strand, within EX28DRAFT_2510 at 2543.108 kb on - strand, within EX28DRAFT_2510 at 2543.174 kb on - strand at 2543.183 kb on + strand at 2543.184 kb on - strand at 2543.184 kb on - strand at 2543.184 kb on - strand at 2543.295 kb on - strand at 2543.668 kb on - strand, within EX28DRAFT_2511 at 2543.668 kb on - strand, within EX28DRAFT_2511 at 2543.668 kb on - strand, within EX28DRAFT_2511 at 2544.056 kb on + strand, within EX28DRAFT_2511 at 2544.209 kb on - strand, within EX28DRAFT_2511 at 2544.357 kb on - strand, within EX28DRAFT_2511 at 2544.539 kb on - strand, within EX28DRAFT_2511 at 2544.601 kb on + strand at 2544.601 kb on + strand at 2544.601 kb on + strand at 2544.601 kb on + strand at 2544.605 kb on + strand at 2544.714 kb on + strand at 2544.725 kb on + strand at 2544.767 kb on + strand at 2544.800 kb on - strand at 2544.816 kb on - strand at 2544.936 kb on + strand, within EX28DRAFT_2512 at 2544.936 kb on + strand, within EX28DRAFT_2512 at 2544.937 kb on - strand, within EX28DRAFT_2512 at 2544.949 kb on + strand, within EX28DRAFT_2512 at 2544.982 kb on + strand, within EX28DRAFT_2512 at 2544.982 kb on + strand, within EX28DRAFT_2512 at 2545.130 kb on + strand, within EX28DRAFT_2513 at 2545.130 kb on + strand, within EX28DRAFT_2513 at 2545.287 kb on + strand at 2545.326 kb on + strand at 2545.354 kb on - strand at 2545.354 kb on - strand at 2545.355 kb on + strand at 2545.430 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with arsenate 5 mM remove 2,542,381 - EX28DRAFT_2509 0.87 +0.1 2,542,500 + +0.3 2,542,501 - -0.3 2,542,501 - +0.1 2,542,550 + +0.1 2,542,672 - EX28DRAFT_2510 0.12 +0.5 2,542,672 - EX28DRAFT_2510 0.12 +1.0 2,542,694 + EX28DRAFT_2510 0.16 +0.4 2,542,694 + EX28DRAFT_2510 0.16 -0.6 2,542,695 - EX28DRAFT_2510 0.16 -0.0 2,542,695 - EX28DRAFT_2510 0.16 +1.0 2,542,695 - EX28DRAFT_2510 0.16 -0.7 2,542,856 + EX28DRAFT_2510 0.42 -0.8 2,542,856 + EX28DRAFT_2510 0.42 +0.4 2,542,857 - EX28DRAFT_2510 0.42 +0.1 2,542,857 - EX28DRAFT_2510 0.42 +0.2 2,543,108 - EX28DRAFT_2510 0.83 +0.6 2,543,174 - +0.7 2,543,183 + -0.6 2,543,184 - +0.1 2,543,184 - +0.6 2,543,184 - -0.2 2,543,295 - -2.7 2,543,668 - EX28DRAFT_2511 0.26 +0.7 2,543,668 - EX28DRAFT_2511 0.26 +0.3 2,543,668 - EX28DRAFT_2511 0.26 +0.1 2,544,056 + EX28DRAFT_2511 0.54 +0.5 2,544,209 - EX28DRAFT_2511 0.64 -0.6 2,544,357 - EX28DRAFT_2511 0.75 +0.2 2,544,539 - EX28DRAFT_2511 0.87 +0.1 2,544,601 + +0.2 2,544,601 + -0.8 2,544,601 + -1.8 2,544,601 + -0.1 2,544,605 + -1.6 2,544,714 + +0.2 2,544,725 + +0.2 2,544,767 + -1.5 2,544,800 - -0.7 2,544,816 - -0.2 2,544,936 + EX28DRAFT_2512 0.64 -0.3 2,544,936 + EX28DRAFT_2512 0.64 -1.2 2,544,937 - EX28DRAFT_2512 0.64 -1.6 2,544,949 + EX28DRAFT_2512 0.70 -0.6 2,544,982 + EX28DRAFT_2512 0.85 +1.0 2,544,982 + EX28DRAFT_2512 0.85 +0.8 2,545,130 + EX28DRAFT_2513 0.48 +0.2 2,545,130 + EX28DRAFT_2513 0.48 -0.1 2,545,287 + -0.4 2,545,326 + +1.5 2,545,354 - +2.0 2,545,354 - +3.0 2,545,355 + +2.3 2,545,430 - +1.3
Or see this region's nucleotide sequence