Experiment: MS_media with arsenate 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1608 and EX28DRAFT_1609 are separated by 139 nucleotides EX28DRAFT_1609 and EX28DRAFT_1610 are separated by 199 nucleotides
EX28DRAFT_1608: EX28DRAFT_1608 - fumarase, class II (EC 4.2.1.2), at 1,624,044 to 1,625,438
_1608
EX28DRAFT_1609: EX28DRAFT_1609 - hydro-lyases, Fe-S type, tartrate/fumarate subfamily, alpha region/hydro-lyases, Fe-S type, tartrate/fumarate subfamily, beta region, at 1,625,578 to 1,627,224
_1609
EX28DRAFT_1610: EX28DRAFT_1610 - mannose-6-phosphate isomerase, type 1 (EC 5.3.1.8), at 1,627,424 to 1,628,599
_1610
Position (kb)
1625
1626
1627
1628 Strain fitness (log2 ratio)
-2
-1
0
1 at 1624.789 kb on + strand, within EX28DRAFT_1608 at 1624.796 kb on + strand, within EX28DRAFT_1608 at 1624.796 kb on + strand, within EX28DRAFT_1608 at 1624.797 kb on - strand, within EX28DRAFT_1608 at 1624.808 kb on + strand, within EX28DRAFT_1608 at 1624.809 kb on - strand, within EX28DRAFT_1608 at 1625.376 kb on + strand at 1625.376 kb on + strand at 1625.376 kb on + strand at 1625.488 kb on - strand at 1625.491 kb on + strand at 1625.491 kb on + strand at 1625.491 kb on + strand at 1625.491 kb on + strand at 1625.492 kb on - strand at 1625.492 kb on - strand at 1625.492 kb on - strand at 1625.579 kb on + strand at 1625.579 kb on + strand at 1625.580 kb on - strand at 1625.734 kb on - strand at 1625.967 kb on - strand, within EX28DRAFT_1609 at 1626.191 kb on + strand, within EX28DRAFT_1609 at 1626.348 kb on - strand, within EX28DRAFT_1609 at 1626.415 kb on + strand, within EX28DRAFT_1609 at 1626.415 kb on + strand, within EX28DRAFT_1609 at 1626.470 kb on + strand, within EX28DRAFT_1609 at 1626.471 kb on - strand, within EX28DRAFT_1609 at 1626.471 kb on - strand, within EX28DRAFT_1609 at 1626.471 kb on - strand, within EX28DRAFT_1609 at 1626.645 kb on - strand, within EX28DRAFT_1609 at 1626.645 kb on - strand, within EX28DRAFT_1609 at 1626.645 kb on - strand, within EX28DRAFT_1609 at 1626.645 kb on - strand, within EX28DRAFT_1609 at 1626.761 kb on + strand, within EX28DRAFT_1609 at 1626.761 kb on + strand, within EX28DRAFT_1609 at 1626.761 kb on + strand, within EX28DRAFT_1609 at 1626.762 kb on - strand, within EX28DRAFT_1609 at 1626.762 kb on - strand, within EX28DRAFT_1609 at 1626.762 kb on - strand, within EX28DRAFT_1609 at 1626.762 kb on - strand, within EX28DRAFT_1609 at 1626.766 kb on - strand, within EX28DRAFT_1609 at 1626.809 kb on + strand, within EX28DRAFT_1609 at 1626.809 kb on + strand, within EX28DRAFT_1609 at 1626.810 kb on - strand, within EX28DRAFT_1609 at 1626.819 kb on - strand, within EX28DRAFT_1609 at 1626.819 kb on - strand, within EX28DRAFT_1609 at 1626.916 kb on - strand, within EX28DRAFT_1609 at 1626.949 kb on - strand, within EX28DRAFT_1609 at 1626.972 kb on - strand, within EX28DRAFT_1609 at 1626.972 kb on - strand, within EX28DRAFT_1609 at 1626.972 kb on - strand, within EX28DRAFT_1609 at 1626.975 kb on + strand, within EX28DRAFT_1609 at 1627.044 kb on + strand, within EX28DRAFT_1609 at 1627.160 kb on + strand at 1627.160 kb on + strand at 1627.237 kb on + strand at 1627.333 kb on + strand at 1627.346 kb on + strand at 1627.359 kb on + strand at 1627.360 kb on - strand at 1627.375 kb on - strand at 1627.395 kb on + strand at 1627.404 kb on - strand at 1627.454 kb on + strand at 1627.491 kb on - strand at 1627.588 kb on - strand, within EX28DRAFT_1610 at 1627.795 kb on + strand, within EX28DRAFT_1610 at 1627.795 kb on + strand, within EX28DRAFT_1610 at 1627.799 kb on + strand, within EX28DRAFT_1610 at 1627.800 kb on - strand, within EX28DRAFT_1610 at 1627.990 kb on + strand, within EX28DRAFT_1610
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with arsenate 5 mM remove 1,624,789 + EX28DRAFT_1608 0.53 +0.2 1,624,796 + EX28DRAFT_1608 0.54 -0.0 1,624,796 + EX28DRAFT_1608 0.54 -0.2 1,624,797 - EX28DRAFT_1608 0.54 +0.5 1,624,808 + EX28DRAFT_1608 0.55 +0.9 1,624,809 - EX28DRAFT_1608 0.55 +0.9 1,625,376 + -0.2 1,625,376 + -2.5 1,625,376 + -1.4 1,625,488 - +0.7 1,625,491 + -0.4 1,625,491 + -0.2 1,625,491 + +0.5 1,625,491 + +0.1 1,625,492 - +1.3 1,625,492 - -2.1 1,625,492 - +0.1 1,625,579 + +0.4 1,625,579 + +0.2 1,625,580 - -0.6 1,625,734 - -0.1 1,625,967 - EX28DRAFT_1609 0.24 +0.3 1,626,191 + EX28DRAFT_1609 0.37 -0.1 1,626,348 - EX28DRAFT_1609 0.47 -0.8 1,626,415 + EX28DRAFT_1609 0.51 -0.3 1,626,415 + EX28DRAFT_1609 0.51 -0.8 1,626,470 + EX28DRAFT_1609 0.54 -0.2 1,626,471 - EX28DRAFT_1609 0.54 -0.4 1,626,471 - EX28DRAFT_1609 0.54 -0.3 1,626,471 - EX28DRAFT_1609 0.54 -0.7 1,626,645 - EX28DRAFT_1609 0.65 +0.0 1,626,645 - EX28DRAFT_1609 0.65 +0.1 1,626,645 - EX28DRAFT_1609 0.65 -0.6 1,626,645 - EX28DRAFT_1609 0.65 +1.4 1,626,761 + EX28DRAFT_1609 0.72 -0.2 1,626,761 + EX28DRAFT_1609 0.72 +0.0 1,626,761 + EX28DRAFT_1609 0.72 -0.6 1,626,762 - EX28DRAFT_1609 0.72 +0.3 1,626,762 - EX28DRAFT_1609 0.72 +0.3 1,626,762 - EX28DRAFT_1609 0.72 -0.4 1,626,762 - EX28DRAFT_1609 0.72 -0.0 1,626,766 - EX28DRAFT_1609 0.72 -0.5 1,626,809 + EX28DRAFT_1609 0.75 -0.2 1,626,809 + EX28DRAFT_1609 0.75 -0.3 1,626,810 - EX28DRAFT_1609 0.75 -1.8 1,626,819 - EX28DRAFT_1609 0.75 +0.2 1,626,819 - EX28DRAFT_1609 0.75 +0.5 1,626,916 - EX28DRAFT_1609 0.81 +0.2 1,626,949 - EX28DRAFT_1609 0.83 +0.2 1,626,972 - EX28DRAFT_1609 0.85 -0.2 1,626,972 - EX28DRAFT_1609 0.85 +0.0 1,626,972 - EX28DRAFT_1609 0.85 -0.5 1,626,975 + EX28DRAFT_1609 0.85 +0.1 1,627,044 + EX28DRAFT_1609 0.89 +0.1 1,627,160 + -0.5 1,627,160 + -0.9 1,627,237 + -0.6 1,627,333 + +0.2 1,627,346 + +1.5 1,627,359 + -0.7 1,627,360 - -0.4 1,627,375 - -0.8 1,627,395 + -0.1 1,627,404 - +0.5 1,627,454 + +0.9 1,627,491 - +1.3 1,627,588 - EX28DRAFT_1610 0.14 -0.0 1,627,795 + EX28DRAFT_1610 0.32 +0.4 1,627,795 + EX28DRAFT_1610 0.32 +1.4 1,627,799 + EX28DRAFT_1610 0.32 +1.4 1,627,800 - EX28DRAFT_1610 0.32 +0.8 1,627,990 + EX28DRAFT_1610 0.48 +1.4
Or see this region's nucleotide sequence