Experiment: MS_media with arsenate 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0299 and EX28DRAFT_0300 are separated by 44 nucleotides EX28DRAFT_0300 and EX28DRAFT_0301 are separated by 13 nucleotides EX28DRAFT_0301 and EX28DRAFT_0302 are separated by 75 nucleotides EX28DRAFT_0302 and EX28DRAFT_0303 are separated by 140 nucleotides
EX28DRAFT_0299: EX28DRAFT_0299 - Acetyltransferases, including N-acetylases of ribosomal proteins, at 260,891 to 261,400
_0299
EX28DRAFT_0300: EX28DRAFT_0300 - TIGR01777 family protein, at 261,445 to 262,338
_0300
EX28DRAFT_0301: EX28DRAFT_0301 - FolB domain, at 262,352 to 262,720
_0301
EX28DRAFT_0302: EX28DRAFT_0302 - Glutathione S-transferase, at 262,796 to 263,425
_0302
EX28DRAFT_0303: EX28DRAFT_0303 - Glutathione S-transferase, at 263,566 to 264,210
_0303
Position (kb) 262
263 Strain fitness (log2 ratio) -1
0
1
2 at 261.438 kb on - strand at 261.443 kb on - strand at 261.443 kb on - strand at 261.454 kb on + strand at 261.454 kb on + strand at 261.476 kb on - strand at 261.476 kb on - strand at 261.545 kb on - strand, within EX28DRAFT_0300 at 261.652 kb on - strand, within EX28DRAFT_0300 at 261.729 kb on + strand, within EX28DRAFT_0300 at 262.088 kb on - strand, within EX28DRAFT_0300 at 262.147 kb on - strand, within EX28DRAFT_0300 at 262.339 kb on - strand at 262.339 kb on - strand at 262.353 kb on + strand at 262.437 kb on + strand, within EX28DRAFT_0301 at 262.437 kb on + strand, within EX28DRAFT_0301 at 262.437 kb on + strand, within EX28DRAFT_0301 at 262.437 kb on + strand, within EX28DRAFT_0301 at 262.438 kb on - strand, within EX28DRAFT_0301 at 262.438 kb on - strand, within EX28DRAFT_0301 at 262.699 kb on + strand at 262.742 kb on + strand at 262.758 kb on + strand at 262.758 kb on + strand at 262.759 kb on - strand at 262.759 kb on - strand at 262.950 kb on + strand, within EX28DRAFT_0302 at 263.005 kb on - strand, within EX28DRAFT_0302 at 263.194 kb on - strand, within EX28DRAFT_0302 at 263.327 kb on + strand, within EX28DRAFT_0302 at 263.327 kb on + strand, within EX28DRAFT_0302 at 263.327 kb on + strand, within EX28DRAFT_0302 at 263.327 kb on + strand, within EX28DRAFT_0302 at 263.328 kb on - strand, within EX28DRAFT_0302 at 263.328 kb on - strand, within EX28DRAFT_0302 at 263.329 kb on + strand, within EX28DRAFT_0302 at 263.329 kb on + strand, within EX28DRAFT_0302 at 263.330 kb on - strand, within EX28DRAFT_0302 at 263.330 kb on - strand, within EX28DRAFT_0302 at 263.330 kb on - strand, within EX28DRAFT_0302 at 263.387 kb on - strand at 263.387 kb on - strand at 263.471 kb on - strand at 263.473 kb on - strand at 263.473 kb on - strand at 263.473 kb on - strand at 263.473 kb on - strand at 263.527 kb on + strand at 263.527 kb on + strand at 263.527 kb on + strand at 263.529 kb on + strand at 263.529 kb on + strand at 263.529 kb on + strand at 263.529 kb on + strand at 263.530 kb on - strand at 263.530 kb on - strand at 263.530 kb on - strand at 263.555 kb on - strand at 263.566 kb on - strand at 263.617 kb on + strand at 263.617 kb on + strand at 263.618 kb on - strand at 263.622 kb on + strand at 263.622 kb on + strand at 263.623 kb on - strand at 263.630 kb on + strand at 263.630 kb on + strand at 263.630 kb on + strand at 263.630 kb on + strand at 263.631 kb on - strand, within EX28DRAFT_0303 at 263.632 kb on + strand, within EX28DRAFT_0303 at 263.632 kb on + strand, within EX28DRAFT_0303 at 263.632 kb on + strand, within EX28DRAFT_0303 at 263.632 kb on + strand, within EX28DRAFT_0303 at 263.633 kb on - strand, within EX28DRAFT_0303
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with arsenate 5 mM remove 261,438 - +0.2 261,443 - -0.1 261,443 - +0.1 261,454 + -0.1 261,454 + -0.2 261,476 - -0.1 261,476 - +0.2 261,545 - EX28DRAFT_0300 0.11 +0.4 261,652 - EX28DRAFT_0300 0.23 +0.5 261,729 + EX28DRAFT_0300 0.32 +0.5 262,088 - EX28DRAFT_0300 0.72 -0.0 262,147 - EX28DRAFT_0300 0.79 -1.1 262,339 - -0.0 262,339 - -1.1 262,353 + +0.3 262,437 + EX28DRAFT_0301 0.23 +0.2 262,437 + EX28DRAFT_0301 0.23 +0.0 262,437 + EX28DRAFT_0301 0.23 -0.5 262,437 + EX28DRAFT_0301 0.23 -0.1 262,438 - EX28DRAFT_0301 0.23 -0.2 262,438 - EX28DRAFT_0301 0.23 +0.9 262,699 + -0.7 262,742 + -0.1 262,758 + -0.2 262,758 + -1.0 262,759 - +0.2 262,759 - +0.0 262,950 + EX28DRAFT_0302 0.24 -0.5 263,005 - EX28DRAFT_0302 0.33 -0.3 263,194 - EX28DRAFT_0302 0.63 -0.6 263,327 + EX28DRAFT_0302 0.84 -0.3 263,327 + EX28DRAFT_0302 0.84 +0.6 263,327 + EX28DRAFT_0302 0.84 -0.1 263,327 + EX28DRAFT_0302 0.84 -0.7 263,328 - EX28DRAFT_0302 0.84 -0.4 263,328 - EX28DRAFT_0302 0.84 -0.0 263,329 + EX28DRAFT_0302 0.85 -0.4 263,329 + EX28DRAFT_0302 0.85 -0.1 263,330 - EX28DRAFT_0302 0.85 -0.3 263,330 - EX28DRAFT_0302 0.85 +0.0 263,330 - EX28DRAFT_0302 0.85 -0.5 263,387 - -0.1 263,387 - -0.6 263,471 - +2.6 263,473 - +0.5 263,473 - +0.5 263,473 - +0.4 263,473 - -0.1 263,527 + +0.6 263,527 + +0.1 263,527 + +0.1 263,529 + +0.0 263,529 + -0.3 263,529 + +0.4 263,529 + -1.2 263,530 - +0.0 263,530 - +0.2 263,530 - -0.3 263,555 - +0.4 263,566 - +0.3 263,617 + -0.1 263,617 + -0.3 263,618 - -0.7 263,622 + +0.5 263,622 + -0.1 263,623 - +0.7 263,630 + +0.1 263,630 + -0.4 263,630 + -0.7 263,630 + +0.2 263,631 - EX28DRAFT_0303 0.10 -0.2 263,632 + EX28DRAFT_0303 0.10 -1.0 263,632 + EX28DRAFT_0303 0.10 -0.0 263,632 + EX28DRAFT_0303 0.10 -0.6 263,632 + EX28DRAFT_0303 0.10 -0.7 263,633 - EX28DRAFT_0303 0.10 +0.4
Or see this region's nucleotide sequence