Experiment: MS_media with arsenate 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0186 and EX28DRAFT_0187 are separated by 73 nucleotides EX28DRAFT_0187 and EX28DRAFT_0188 are separated by 38 nucleotides EX28DRAFT_0188 and EX28DRAFT_0189 are separated by 24 nucleotides
EX28DRAFT_0186: EX28DRAFT_0186 - Predicted P-loop ATPase fused to an acetyltransferase, at 135,308 to 137,254
_0186
EX28DRAFT_0187: EX28DRAFT_0187 - Predicted esterase, at 137,328 to 138,023
_0187
EX28DRAFT_0188: EX28DRAFT_0188 - Uncharacterised protein family (UPF0370), at 138,062 to 138,259
_0188
EX28DRAFT_0189: EX28DRAFT_0189 - succinyldiaminopimelate desuccinylase (EC 3.5.1.18), at 138,284 to 139,411
_0189
Position (kb)
137
138
139 Strain fitness (log2 ratio)
-1
0
1 at 136.384 kb on - strand, within EX28DRAFT_0186 at 136.384 kb on - strand, within EX28DRAFT_0186 at 136.385 kb on + strand, within EX28DRAFT_0186 at 136.665 kb on + strand, within EX28DRAFT_0186 at 136.665 kb on + strand, within EX28DRAFT_0186 at 136.665 kb on + strand, within EX28DRAFT_0186 at 136.753 kb on + strand, within EX28DRAFT_0186 at 136.839 kb on - strand, within EX28DRAFT_0186 at 137.264 kb on + strand at 137.312 kb on + strand at 137.312 kb on + strand at 137.312 kb on + strand at 137.313 kb on - strand at 137.380 kb on + strand at 137.402 kb on - strand, within EX28DRAFT_0187 at 137.421 kb on - strand, within EX28DRAFT_0187 at 137.913 kb on - strand, within EX28DRAFT_0187 at 137.962 kb on - strand at 137.962 kb on - strand at 138.017 kb on + strand at 138.063 kb on + strand at 138.241 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with arsenate 5 mM remove 136,384 - EX28DRAFT_0186 0.55 -0.1 136,384 - EX28DRAFT_0186 0.55 -0.2 136,385 + EX28DRAFT_0186 0.55 -1.1 136,665 + EX28DRAFT_0186 0.70 +0.7 136,665 + EX28DRAFT_0186 0.70 -0.2 136,665 + EX28DRAFT_0186 0.70 -0.7 136,753 + EX28DRAFT_0186 0.74 +0.0 136,839 - EX28DRAFT_0186 0.79 +1.1 137,264 + -0.0 137,312 + -0.9 137,312 + -0.2 137,312 + +0.0 137,313 - -0.5 137,380 + -0.1 137,402 - EX28DRAFT_0187 0.11 -0.7 137,421 - EX28DRAFT_0187 0.13 -0.5 137,913 - EX28DRAFT_0187 0.84 -0.1 137,962 - -0.5 137,962 - +0.6 138,017 + -1.0 138,063 + -0.3 138,241 - +0.8
Or see this region's nucleotide sequence