Experiment: Glycolic Acid (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE40_RS20300 and ABIE40_RS20305 are separated by 9 nucleotides ABIE40_RS20305 and ABIE40_RS20310 overlap by 4 nucleotides ABIE40_RS20310 and ABIE40_RS20315 overlap by 1 nucleotides ABIE40_RS20315 and ABIE40_RS20320 are separated by 116 nucleotides ABIE40_RS20320 and ABIE40_RS20325 overlap by 14 nucleotides
ABIE40_RS20300: ABIE40_RS20300 - heavy metal translocating P-type ATPase, at 166,309 to 168,828
_RS20300
ABIE40_RS20305: ABIE40_RS20305 - Cu(I)-responsive transcriptional regulator, at 168,838 to 169,233
_RS20305
ABIE40_RS20310: ABIE40_RS20310 - type II toxin-antitoxin system VapC family toxin, at 169,230 to 169,661
_RS20310
ABIE40_RS20315: ABIE40_RS20315 - type II toxin-antitoxin system VapB family antitoxin, at 169,661 to 169,900
_RS20315
ABIE40_RS20320: ABIE40_RS20320 - sigma-70 family RNA polymerase sigma factor, at 170,017 to 170,556
_RS20320
ABIE40_RS20325: ABIE40_RS20325 - anti-sigma factor, at 170,543 to 171,310
_RS20325
Position (kb) 169
170 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 168.681 kb on + strand at 168.681 kb on + strand at 168.681 kb on + strand at 168.682 kb on - strand at 168.719 kb on - strand at 168.827 kb on - strand at 168.892 kb on + strand, within ABIE40_RS20305 at 168.895 kb on + strand, within ABIE40_RS20305 at 168.895 kb on + strand, within ABIE40_RS20305 at 168.949 kb on + strand, within ABIE40_RS20305 at 168.950 kb on - strand, within ABIE40_RS20305 at 168.950 kb on - strand, within ABIE40_RS20305 at 168.955 kb on - strand, within ABIE40_RS20305 at 169.042 kb on + strand, within ABIE40_RS20305 at 169.042 kb on + strand, within ABIE40_RS20305 at 169.043 kb on - strand, within ABIE40_RS20305 at 169.043 kb on - strand, within ABIE40_RS20305 at 169.098 kb on + strand, within ABIE40_RS20305 at 169.143 kb on + strand, within ABIE40_RS20305 at 169.144 kb on - strand, within ABIE40_RS20305 at 169.257 kb on - strand at 169.321 kb on + strand, within ABIE40_RS20310 at 169.321 kb on + strand, within ABIE40_RS20310 at 169.322 kb on - strand, within ABIE40_RS20310 at 169.322 kb on - strand, within ABIE40_RS20310 at 169.322 kb on - strand, within ABIE40_RS20310 at 169.519 kb on + strand, within ABIE40_RS20310 at 169.520 kb on - strand, within ABIE40_RS20310 at 169.520 kb on - strand, within ABIE40_RS20310 at 169.520 kb on - strand, within ABIE40_RS20310 at 169.520 kb on - strand, within ABIE40_RS20310 at 169.520 kb on - strand, within ABIE40_RS20310 at 169.632 kb on - strand at 169.641 kb on + strand at 169.642 kb on - strand at 169.642 kb on - strand at 169.828 kb on + strand, within ABIE40_RS20315 at 169.889 kb on - strand at 169.923 kb on - strand at 169.929 kb on + strand at 169.932 kb on - strand at 169.987 kb on - strand at 170.006 kb on + strand at 170.006 kb on + strand at 170.006 kb on + strand at 170.007 kb on - strand at 170.007 kb on - strand at 170.007 kb on - strand at 170.007 kb on - strand at 170.007 kb on - strand at 170.008 kb on + strand at 170.009 kb on - strand at 170.009 kb on - strand at 170.180 kb on + strand, within ABIE40_RS20320 at 170.217 kb on + strand, within ABIE40_RS20320 at 170.404 kb on + strand, within ABIE40_RS20320 at 170.404 kb on + strand, within ABIE40_RS20320 at 170.404 kb on + strand, within ABIE40_RS20320 at 170.405 kb on - strand, within ABIE40_RS20320 at 170.443 kb on - strand, within ABIE40_RS20320 at 170.526 kb on - strand at 170.585 kb on + strand at 170.585 kb on + strand at 170.585 kb on + strand at 170.633 kb on + strand, within ABIE40_RS20325 at 170.633 kb on + strand, within ABIE40_RS20325 at 170.722 kb on + strand, within ABIE40_RS20325 at 170.722 kb on + strand, within ABIE40_RS20325 at 170.780 kb on + strand, within ABIE40_RS20325 at 170.797 kb on + strand, within ABIE40_RS20325 at 170.797 kb on + strand, within ABIE40_RS20325 at 170.826 kb on + strand, within ABIE40_RS20325 at 170.854 kb on + strand, within ABIE40_RS20325
Per-strain Table
Position Strand Gene LocusTag Fraction Glycolic Acid (C) remove 168,681 + +0.0 168,681 + +0.2 168,681 + -1.0 168,682 - -0.1 168,719 - -0.9 168,827 - -0.1 168,892 + ABIE40_RS20305 0.14 -0.1 168,895 + ABIE40_RS20305 0.14 -1.7 168,895 + ABIE40_RS20305 0.14 -0.8 168,949 + ABIE40_RS20305 0.28 +0.8 168,950 - ABIE40_RS20305 0.28 +0.4 168,950 - ABIE40_RS20305 0.28 -0.3 168,955 - ABIE40_RS20305 0.30 +1.0 169,042 + ABIE40_RS20305 0.52 -1.4 169,042 + ABIE40_RS20305 0.52 +1.1 169,043 - ABIE40_RS20305 0.52 -0.8 169,043 - ABIE40_RS20305 0.52 -1.4 169,098 + ABIE40_RS20305 0.66 -0.6 169,143 + ABIE40_RS20305 0.77 +0.7 169,144 - ABIE40_RS20305 0.77 +1.2 169,257 - -0.1 169,321 + ABIE40_RS20310 0.21 +2.5 169,321 + ABIE40_RS20310 0.21 -0.6 169,322 - ABIE40_RS20310 0.21 -0.2 169,322 - ABIE40_RS20310 0.21 -0.1 169,322 - ABIE40_RS20310 0.21 +0.5 169,519 + ABIE40_RS20310 0.67 -0.9 169,520 - ABIE40_RS20310 0.67 +0.5 169,520 - ABIE40_RS20310 0.67 -0.0 169,520 - ABIE40_RS20310 0.67 +0.1 169,520 - ABIE40_RS20310 0.67 -0.1 169,520 - ABIE40_RS20310 0.67 +0.0 169,632 - -0.3 169,641 + -0.4 169,642 - -1.3 169,642 - +0.4 169,828 + ABIE40_RS20315 0.70 -1.6 169,889 - +0.4 169,923 - -1.1 169,929 + -0.1 169,932 - -0.2 169,987 - -2.1 170,006 + -0.3 170,006 + -0.5 170,006 + -0.2 170,007 - -0.4 170,007 - -2.7 170,007 - -1.2 170,007 - +0.8 170,007 - -3.1 170,008 + -0.4 170,009 - +1.1 170,009 - -0.1 170,180 + ABIE40_RS20320 0.30 -1.3 170,217 + ABIE40_RS20320 0.37 -0.0 170,404 + ABIE40_RS20320 0.72 +0.3 170,404 + ABIE40_RS20320 0.72 -0.2 170,404 + ABIE40_RS20320 0.72 +0.5 170,405 - ABIE40_RS20320 0.72 -0.4 170,443 - ABIE40_RS20320 0.79 -0.6 170,526 - +0.4 170,585 + -1.5 170,585 + +0.1 170,585 + +1.0 170,633 + ABIE40_RS20325 0.12 -0.6 170,633 + ABIE40_RS20325 0.12 +0.5 170,722 + ABIE40_RS20325 0.23 -0.4 170,722 + ABIE40_RS20325 0.23 -0.4 170,780 + ABIE40_RS20325 0.31 +2.3 170,797 + ABIE40_RS20325 0.33 -0.7 170,797 + ABIE40_RS20325 0.33 -0.4 170,826 + ABIE40_RS20325 0.37 -0.5 170,854 + ABIE40_RS20325 0.40 -2.5
Or see this region's nucleotide sequence