Experiment: Glycolic Acid (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE40_RS17585 and ABIE40_RS17590 are separated by 26 nucleotides ABIE40_RS17590 and ABIE40_RS17595 overlap by 4 nucleotides
ABIE40_RS17585: ABIE40_RS17585 - DUF2125 domain-containing protein, at 3,512,648 to 3,513,649
_RS17585
ABIE40_RS17590: ABIE40_RS17590 - prephenate/arogenate dehydrogenase family protein, at 3,513,676 to 3,514,608
_RS17590
ABIE40_RS17595: ABIE40_RS17595 - histidinol-phosphate transaminase, at 3,514,605 to 3,515,714
_RS17595
Position (kb)
3513
3514
3515 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3512.726 kb on + strand at 3512.727 kb on - strand at 3512.727 kb on - strand at 3512.727 kb on - strand at 3512.728 kb on + strand at 3512.744 kb on + strand at 3512.745 kb on - strand at 3512.945 kb on + strand, within ABIE40_RS17585 at 3512.946 kb on - strand, within ABIE40_RS17585 at 3512.946 kb on - strand, within ABIE40_RS17585 at 3512.946 kb on - strand, within ABIE40_RS17585 at 3512.959 kb on + strand, within ABIE40_RS17585 at 3513.122 kb on + strand, within ABIE40_RS17585 at 3513.123 kb on - strand, within ABIE40_RS17585 at 3513.123 kb on - strand, within ABIE40_RS17585 at 3513.223 kb on + strand, within ABIE40_RS17585 at 3513.223 kb on + strand, within ABIE40_RS17585 at 3513.647 kb on + strand at 3513.648 kb on - strand at 3513.648 kb on - strand at 3513.960 kb on - strand, within ABIE40_RS17590 at 3514.020 kb on - strand, within ABIE40_RS17590 at 3514.020 kb on - strand, within ABIE40_RS17590 at 3514.020 kb on - strand, within ABIE40_RS17590 at 3514.272 kb on + strand, within ABIE40_RS17590 at 3514.272 kb on + strand, within ABIE40_RS17590 at 3514.273 kb on - strand, within ABIE40_RS17590 at 3514.396 kb on + strand, within ABIE40_RS17590 at 3514.396 kb on + strand, within ABIE40_RS17590 at 3514.445 kb on + strand, within ABIE40_RS17590 at 3514.545 kb on - strand at 3514.608 kb on + strand at 3514.608 kb on + strand at 3514.608 kb on + strand at 3514.608 kb on + strand at 3514.609 kb on - strand at 3514.609 kb on - strand at 3514.609 kb on - strand at 3514.613 kb on + strand at 3514.613 kb on + strand at 3514.614 kb on - strand at 3514.622 kb on + strand at 3514.623 kb on - strand at 3514.623 kb on - strand at 3514.821 kb on - strand, within ABIE40_RS17595 at 3514.821 kb on - strand, within ABIE40_RS17595 at 3515.005 kb on + strand, within ABIE40_RS17595 at 3515.005 kb on + strand, within ABIE40_RS17595 at 3515.005 kb on + strand, within ABIE40_RS17595 at 3515.006 kb on - strand, within ABIE40_RS17595 at 3515.038 kb on + strand, within ABIE40_RS17595 at 3515.038 kb on + strand, within ABIE40_RS17595 at 3515.038 kb on + strand, within ABIE40_RS17595 at 3515.039 kb on - strand, within ABIE40_RS17595 at 3515.125 kb on + strand, within ABIE40_RS17595 at 3515.125 kb on + strand, within ABIE40_RS17595 at 3515.125 kb on + strand, within ABIE40_RS17595 at 3515.126 kb on - strand, within ABIE40_RS17595 at 3515.135 kb on - strand, within ABIE40_RS17595 at 3515.209 kb on + strand, within ABIE40_RS17595 at 3515.209 kb on + strand, within ABIE40_RS17595 at 3515.209 kb on + strand, within ABIE40_RS17595 at 3515.210 kb on - strand, within ABIE40_RS17595 at 3515.210 kb on - strand, within ABIE40_RS17595 at 3515.210 kb on - strand, within ABIE40_RS17595 at 3515.210 kb on - strand, within ABIE40_RS17595 at 3515.356 kb on + strand, within ABIE40_RS17595 at 3515.407 kb on + strand, within ABIE40_RS17595
Per-strain Table
Position Strand Gene LocusTag Fraction Glycolic Acid (C) remove 3,512,726 + -1.7 3,512,727 - -0.2 3,512,727 - +0.6 3,512,727 - -0.9 3,512,728 + +0.5 3,512,744 + -1.2 3,512,745 - -1.1 3,512,945 + ABIE40_RS17585 0.30 +0.2 3,512,946 - ABIE40_RS17585 0.30 +0.2 3,512,946 - ABIE40_RS17585 0.30 -0.5 3,512,946 - ABIE40_RS17585 0.30 -0.4 3,512,959 + ABIE40_RS17585 0.31 -0.4 3,513,122 + ABIE40_RS17585 0.47 -1.1 3,513,123 - ABIE40_RS17585 0.47 -0.3 3,513,123 - ABIE40_RS17585 0.47 +1.1 3,513,223 + ABIE40_RS17585 0.57 -0.5 3,513,223 + ABIE40_RS17585 0.57 -0.0 3,513,647 + -0.0 3,513,648 - +0.9 3,513,648 - -0.9 3,513,960 - ABIE40_RS17590 0.30 -2.4 3,514,020 - ABIE40_RS17590 0.37 -1.4 3,514,020 - ABIE40_RS17590 0.37 -0.1 3,514,020 - ABIE40_RS17590 0.37 -0.9 3,514,272 + ABIE40_RS17590 0.64 -1.5 3,514,272 + ABIE40_RS17590 0.64 -2.2 3,514,273 - ABIE40_RS17590 0.64 -1.5 3,514,396 + ABIE40_RS17590 0.77 +0.5 3,514,396 + ABIE40_RS17590 0.77 -1.1 3,514,445 + ABIE40_RS17590 0.82 -1.1 3,514,545 - -0.5 3,514,608 + -2.1 3,514,608 + -1.5 3,514,608 + -2.2 3,514,608 + -2.4 3,514,609 - -0.4 3,514,609 - +1.2 3,514,609 - +1.0 3,514,613 + -1.4 3,514,613 + -0.9 3,514,614 - -0.2 3,514,622 + -1.5 3,514,623 - +1.0 3,514,623 - +0.8 3,514,821 - ABIE40_RS17595 0.19 -3.5 3,514,821 - ABIE40_RS17595 0.19 -3.2 3,515,005 + ABIE40_RS17595 0.36 +0.5 3,515,005 + ABIE40_RS17595 0.36 -2.8 3,515,005 + ABIE40_RS17595 0.36 -1.1 3,515,006 - ABIE40_RS17595 0.36 -0.5 3,515,038 + ABIE40_RS17595 0.39 -2.5 3,515,038 + ABIE40_RS17595 0.39 -1.7 3,515,038 + ABIE40_RS17595 0.39 +1.5 3,515,039 - ABIE40_RS17595 0.39 +0.5 3,515,125 + ABIE40_RS17595 0.47 -2.5 3,515,125 + ABIE40_RS17595 0.47 -0.1 3,515,125 + ABIE40_RS17595 0.47 -0.9 3,515,126 - ABIE40_RS17595 0.47 -1.7 3,515,135 - ABIE40_RS17595 0.48 -2.9 3,515,209 + ABIE40_RS17595 0.54 -1.9 3,515,209 + ABIE40_RS17595 0.54 -1.4 3,515,209 + ABIE40_RS17595 0.54 +1.5 3,515,210 - ABIE40_RS17595 0.55 -3.5 3,515,210 - ABIE40_RS17595 0.55 -1.4 3,515,210 - ABIE40_RS17595 0.55 -3.8 3,515,210 - ABIE40_RS17595 0.55 -3.9 3,515,356 + ABIE40_RS17595 0.68 -2.0 3,515,407 + ABIE40_RS17595 0.72 -3.2
Or see this region's nucleotide sequence